Isolation and characterization of endophytic bacteria isolated from the leaves of the common bean (Phaseolus vulgaris)

Abstract

The common bean is one of the most important legumes in the human diet, but little is known about the endophytic bacteria associated with the leaves of this plant. The objective of this study was to characterize the culturable endophytic bacteria of common bean (Phaseolus vulgaris) leaves from three different cultivars (Vermelhinho, Talismã, and Ouro Negro) grown under the same field conditions. The density of endophytic populations varied from 4.5 x 10 2 to 2.8 x 10 3 CFU g The isolates from the Talismã cultivar were less diverse than the isolates derived from the other two cultivars. The results of this work indicate that the cultivar of the plant may contribute to the structure of the endophytic community associated with the common bean. This is the first report of endophytic bacteria from the leaves of P. vulgaris cultivars. Future studies will determine the potential application of these isolates in biological control, growth promotion and enzyme production for biotechnology.

Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata

“…The fern, E. arvense (Figure 1), was collected from three different locations at the campus of Yeungnam University in 2014 (Gyeongsan, Republic of Korea), after which the endophytic bacteria were isolated by standard isolation protocol 38 . Briefly, 2 grams of the leaves, stems and roots of E. arvense were washed with running tap water, sterilized with 70% ethanol for 60 sec, 2% sodium hypochlorite for 90 sec and 100% ethanol for 30 sec, consecutively, and then washed five times with sterile ddH 2 O.…”

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

“…In Brazil, biotechnological research has targeted the diversity of microorganisms because of the potential for discovery of new bioactive compounds or bioprocesses (Schulz and Boyle, 2005;de Oliveira Costa et al, 2012;García et al, 2012b;Rhoden et al, 2012;Miguel et al, 2013;Polonio et al, 2015). Studies on microbiota associated with plants are important to understand and predict the spatial distribution of microbial communities and the ecosystem responses to global climate change (Singh et al, 2010).…”

RNA applications for endophytic research

“…Rhoden et al (2015) identified 16 endophytic bacteria living on leaves of Trichilia elegans (Meliaceae), using sequencing and phylogenetic analyses. Several studies have isolated and identified endophytic bacteria of medicinal plants with the 16S rRNA gene used as a tool in molecular identification (Compant et al, 2010;de Oliveira Costa et al, 2012;Miguel et al, 2013;Castro et al, 2014;Rhoden et al, 2015).…”