Isolation and characterization of human cervical-mucus glycoproteins

Carlstedt, Ingemar; Lindgren, Hanna; Sheehan, John K.; Ulmsten, Ulf; Wingerup, L.

doi:10.1042/bj2110013

Cited by 223 publications

(204 citation statements)

References 30 publications

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“…4). While proteins and glycoproteins in general have densities not exceeding 1.3 g/ml, this value is in agreement with the known characteristics of mucus glycoproteins [17]. A similar buoyant density has been reported for a Ca19-9 active serum mucin [18].…”

Section: Purification Of the Ca 125 And Ca 19-9 Active Mucus Glycoprosupporting

confidence: 71%

Ca 125 and Ca 19–9: two cancer‐associated sialylsaccharide antigens on a mucus glycoprotein from human milk

Hanisch

Uhlenbruck

Dienst

et al. 1985

European Journal of Biochemistry

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The cancer-associated antigens Ca 125 and Ca 19-9 were demonstrated by radioimmunoassay to form structural units of a mucus glycoprotein in human milk taken from healthy women four days after parturition. The glycoprotein precipitated with the casein fraction at pH 4.6 and was completely absent in the whey as judged from Ca 19-9 assay. It could be effectively enriched by phenol-saline extraction from soluble milk proteins and further purified by gel filtration on Sephacryl S300 and Sephacryl S400.The active component with a bouyant density of 1.41 g/ml in isopycnic density gradient centrifugation (CsCl) shared common physico-chemical and chemical characteristics of mucus glycoproteins. Carbohydrates representing about 68% by weight were conjugated to protein by alkali-labile linkages, exclusively and were essentially free of D-mannose.Activities of Ca 125 and Ca 19-9 were both destroyed by treatment with periodate, mild alkali or neuraminidase suggesting the antigens are sialylated saccharides bound to protein by alkali-labile linkages. The fraction of monosialylated saccharide alditols isolated after reductive p-elimination from the mucus glycoprotein was shown to inhibit monoclonal antibodies anti-(Ca 125) and anti-(Ca 19-9) in radioimmunoassay.

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Section: Purification Of the Ca 125 And Ca 19-9 Active Mucus Glycoprosupporting

confidence: 71%

Ca 125 and Ca 19–9: two cancer‐associated sialylsaccharide antigens on a mucus glycoprotein from human milk

Hanisch

Uhlenbruck

Dienst

et al. 1985

European Journal of Biochemistry

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“…At the trans‐golgi compartment, the addition of sialic acid or GalNAc will cease glycan extension 31. The end result is a highly decorated protein dimer with nearly 80% of the mass accounted for by glycans 32. These glycan chains are highly heterogeneous in chain length and composition even at homeostasis.…”

Section: Muc2 Biosynthesismentioning

confidence: 99%

A sticky end for gastrointestinal helminths; the role of the mucus barrier

Sharpe

Thornton

Grencis

2018

Parasite Immunology

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SummaryGastrointestinal (GI) nematodes are a group of successful multicellular parasites that have evolved to coexist within the intestinal niche of multiple species. It is estimated that over 10% of the world's population are chronically infected by GI nematodes, making this group of parasitic nematodes a major burden to global health. Despite the large number of affected individuals, there are few effective treatments to eradicate these infections. Research into GI nematode infections has primarily focused on defining the immunological and pathological consequences on host protection. One important but neglected aspect of host protection is mucus, and the concept that mucus is just a simple barrier is no longer tenable. In fact, mucus is a highly regulated and dynamic‐secreted matrix, underpinned by a physical hydrated network of highly glycosylated mucins, which is increasingly recognized to have a key protective role against GI nematode infections. Unravelling the complex interplay between mucins, the underlying epithelium and immune cells during infection are a major challenge and are required to fully define the protective role of the mucus barrier. This review summarizes the current state of knowledge on mucins and the mucus barrier during GI nematode infections, with particular focus on murine models of infection.

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“…Other groups have included 4 -6 M guanidinium hydrochloride in the extraction solution and in the CsCl gradient [15,16]. The problem with this is that it is a chaotropic agent and will denature the mucins and reduce their ability to form a gel.…”

Section: Intestinal Mucus Collection and Purificationmentioning

confidence: 99%