Isolation and characterization of lectin from the leaves of Euphorbia tithymaloides (L.)

Jawade, Aruna A.; Pingle, Shubhangi K.; Tumane, Rajani G.; Sharma, Anvita S.; Ramteke, Archana S.; Jain, Ruchika

doi:10.22271/tpr.2016.v3.i3.083

Cited by 10 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that, phytolectins have property to specifically bind glycoconjugates on the cell surface and agglutinates RBCs of human as well as animals. Plant extract also agglutinates some of the animal erythrocytes which is supported by study of Euphorbia tithymaloids by Jawade et al (2016), Ahmed & Cahtterjee (1987) and with Tridax procumbans by Ramteke & Patil (2005).…”

Section: Discussionmentioning

confidence: 72%

Isolation and characterization of hemagglutinins from Clerodendrum phlomidis L.f.

Khagar¹,

Khagar²,

Pingle³

et al. 2017

Trop. Plant Res.

Self Cite

View full text Add to dashboard Cite

Abstract:Lectins from the leaves, fruit, calyx and stem of Clerodendrum phlomidis plant were isolated after screening of total 54 plants from central India. The crude extract of leaves, fruit, calyx and stem of C. phlomidis plant was purified by ammonium sulfate precipitation, followed by dialysis. The isolated lectins of C. phlomidis plant were able to agglutinate human as well as dog, hen, goat mouse, mice and fish erythrocytes. The isolated lectins were found to be lactose specific. The pH stability of C. phlomidis leaves lectins was between 4-10 pH, for fruit lectins it was 5-7 pH, and for calyx and stem lectins it was found stable in between 4 and 8 pH. The calyx and stem lectins were active until 60ºC, fruit has shown activity upto 50ºC and in case of leaves, it was found maximum 40ºC. Beyond their respective optimum temperature and pH range, all the lectins were unable to agglutinate erythrocytes. Hemagglutination activity of C. phlomidis plant lectins was also checked in the presence of different metal ions and it was found that it was partially inhibited by aluminium metal ion.

show abstract

Section: Discussionmentioning

confidence: 72%

Isolation and characterization of hemagglutinins from Clerodendrum phlomidis L.f.

Khagar¹,

Khagar²,

Pingle³

et al. 2017

Trop. Plant Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The solution was incubated for 1 h at room temperature and observed visually. Positive results of the agglutination reaction were indicated by a uniform red color in the solution, while negative results showed a red dot precipitate [18].…”

Section: Hemagglutination Test For Lectin Identificationmentioning

confidence: 99%

“…The electrophoresis procedure refers to Jiang et al [17] and Jawade et al [18] with slight modifications. Crude extract samples, A1, B1, B2, C1, and C2, were mixed with Coomassie brilliant blue loading dye in a ratio of 5:1 and heated in a water bath at 95-100℃ for 5 min.…”

Section: B Sds-page Electrophoresismentioning

confidence: 99%

Optimization strategy for purification and isolation of active lectin from Colocasia esculenta L. Schott rhizome

Desmiaty,

Chaidir,

Utami

et al. 2024

J App Biol Biotech

View full text Add to dashboard Cite

Lectins are non-enzymatic proteins that attach to carbohydrates and exhibit diverse biological activities and are found in Colocasia esculenta rhizomes. This study determined the optimal conditions for purifying and isolating lectin from C. esculenta rhizome. The methods involved the preparation of crude extracts with variations of salting in, salting in followed by salting out, and precipitation with phosphate buffer saline (PBS), then all extracts were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Purification and isolation were carried out using ion exchange chromatography with pH variations of 8.3, 7.2, and 9.6. The isolates were evaluated using the bicinchoninic acid method, SDS-PAGE, and hemagglutination test. The results of SDS-PAGE showed that the best extraction method involved the use of PBS as indicated by the presence of a prominent band at ~12.5kDa. During pH optimization for purification, the best isolate was obtained at pH 9.6. This was observed by the appearance of a single band with ~12.5kDa MW on 12% SDS-PAGE gel and occurrence of an agglutination reaction at the hemagglutination stage. The total protein content of lectins ranged from 1284.83 to 2947.33 μg/mL. The C. esculenta rhizome contains lectins as active protein compounds. The best isolation condition is by extraction with PBS solvent and purification using ion exchange chromatography at pH 9.6.

show abstract

“…It is also crucial to note the activity of proteins present in the Euphorbiaceae latex. Euphorbia trigona latex is high in lectin-content, which is responsible for precipitation and agglutination in cells through binding specifically and reversibly to carbohydrates ( Jawade et al., 2016 ). Three of those lectins have been characterized and their RNA N-glycosidase activity was tested on several human cancer cell lines, including HeLa, A549, HCT116, HL-60, human colorectal HT-29.…”

Section: Anticancer Properties Of Latex-bearing Plant Extractsmentioning

confidence: 99%

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

Mazur

Bałdysz²,

Warowicka³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Latex-bearing plants have been in the research spotlight for the past couple of decades. Since ancient times their extracts have been used in folk medicine to treat various illnesses. Currently they serve as promising candidates for cancer treatment. Up to date there have been several in vitro and in vivo studies related to the topic of cytotoxicity and anticancer activity of extracts from latex-bearing plants towards various cell types. The number of clinical studies still remains scarce, however, over the years the number is systematically increasing. To the best of our knowledge, the scientific community is still lacking in a recent review summarizing the research on the topic of cytotoxicity and anticancer activity of latex-bearing plant extracts. Therefore, the aim of this paper is to review the current knowledge on in vitro and in vivo studies, which focus on the cytotoxicity and anticancer activities of latex-bearing plants. The vast majority of the studies are in vitro, however, the interest in this topic has resulted in the substantial growth of the number of in vivo studies, leading to a promising number of plant species whose latex can potentially be tested in clinical trials. The paper is divided into sections, each of them focuses on specific latex-bearing plant family representatives and their potential anticancer activity, which in some instances is comparable to that induced by commonly used therapeutics currently available on the market. The cytotoxic effect of the plant’s crude latex, its fractions or isolated compounds, is analyzed, along with a study of cell apoptosis, chromatin condensation, DNA damage, changes in gene regulation and morphology changes, which can be observed in cell post plant extract addition. The in vivo studies go beyond the molecular level by showing significant reduction of the tumor growth and volume in animal models. Additionally, we present data regarding plant-mediated biosynthesis of nanoparticles, which is regarded as a new branch in plant latex research. It is solely based on the green-synthesis approach, which presents an interesting alternative to chemical-based nanoparticle synthesis. We have analyzed the cytotoxic effect of these particles on cells. Data regarding the cytotoxicity of such particles raises their potential to be involved in the design of novel cancer therapies, which further underlines the significance of latex-bearing plants in biotechnology. Throughout the course of this review, we concluded that plant latex is a rich source of many compounds, which can be further investigated and applied in the design of anticancer pharmaceuticals. The molecules, to which this cytotoxic effect can be attributed, include alkaloids, flavonoids, tannins, terpenoids, proteases, nucleases and many novel compounds, which still remain to be characterized. They have been studied extensively in both in vitro and in vivo studies, which provide an excellent starting point for their rapid transfer to clinical studies in the near future. The comprehensive study of molecules from latex-bearing plants can result in finding a promising alternative to several pharmaceuticals on the market and help unravel the molecular mode of action of latex-based preparations.

show abstract

Isolation and characterization of lectin from the leaves of Euphorbia tithymaloides (L.)

Cited by 10 publications

References 12 publications

Isolation and characterization of hemagglutinins from Clerodendrum phlomidis L.f.

Isolation and characterization of hemagglutinins from Clerodendrum phlomidis L.f.

Optimization strategy for purification and isolation of active lectin from Colocasia esculenta L. Schott rhizome

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

Contact Info

Product

Resources

About