Isolation and characterization of Macrophomina phaseolina isolates prevailing in Sindh, Pakistan

Wagan, K. H.; Khaskheli, Muhammad Ibrahim; Hajano, Jamal-U-Ddin; Lanjar, Abdul Ghani

doi:10.19045/bspab.2018.700152

Cited by 2 publications

(2 citation statements)

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“…The morphological and cultural studies revealed that M. phaseolina isolates were grouped into different categories based on growth pattern (appressed, fluffy, and velvety), growth rate, colony color (light grey, grey, and black), aerial mycelium (present and absent), sclerotial initiation time, sclerotial number (less, moderate, and high), sclerotia size (small, medium, and large) and sclerotia shape (round, ovoid, and irregular). Similarly, earlier studies reported that the M. phaseolina associated with different crops also grouped the isolates into different categories based on diameter growth on medium, colony color, mycelial characteristics, the morphology of the sclerotia, and sclerotial initiation time ( Suriachandraselvan and Seetharaman, 2003 ; Sharma et al., 2004 ; Gupta et al., 2012 ; Sharma et al., 2012 ; Tanaji et al., 2017 ; Wagan et al., 2018 ; Thirunarayanan et al., 2020 ; Chiranjeevi et al., 2021 ).…”

Section: Discussionmentioning

confidence: 76%

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Prevalence of groundnut dry root rot (Macrophomina phaseolina (Tassi) Goid.) and its pathogenic variability in Southern India

Pamala,

Jayalakshmi,

Vemana

et al. 2023

Front. Fungal Biol.

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Macrophomina phaseolina is the most devastating and emerging threat to groundnut production in India. An increase in average temperature and inconsistent rainfalls resulting from changing climatic conditions are strongly believed to aggravate the disease and cause severe yield losses. The present study aims to conduct a holistic survey to assess the prevalence and incidence of dry root rot of groundnut in major groundnut growing regions of Southern India, viz., Andhra Pradesh, Telangana, Karnataka, and Tamil Nadu. Furthermore, the pathogenic variability was determined using different assays such as morphological, cultural, pathogenic, and molecular assays. Results indicate that disease incidence in surveyed locations ranged from 8.06 to 20.61%. Both temperature and rainfall played a major role in increasing the disease incidence. The pathogenic variability of M. phaseolina isolates differed significantly, based on the percent disease incidence induced on cultivars of JL-24 groundnut and K-6 groundnut. Morphological variations in terms of growth pattern, culture color, sclerotia number, and sclerotia size were observed. The molecular characterization of M. phaseolina isolates done by ITS rDNA region using ITS1 and ITS4 primers yielded approximately 600 bp PCR amplicons, sequenced and deposited in GenBank (NCBI). Molecular variability analysis using SSR primers indicated the genetic variation among the isolates collected from different states. The present investigation revealed significant variations in pathogenic variability among isolates of M. phaseolina and these may be considered important in disease management and the development of resistant cultivars against groundnut dry root rot disease.

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Section: Discussionmentioning

confidence: 76%

“…the morphology of the sclerotia, and sclerotial initiation time (Suriachandraselvan and Seetharaman, 2003;Sharma et al, 2004;Gupta et al, 2012;Sharma et al, 2012;Tanaji et al, 2017;Wagan et al, 2018;Thirunarayanan et al, 2020;Chiranjeevi et al, 2021).…”

Section: Discussionmentioning

confidence: 99%