Isolation and characterization of mastitis pathogens and milk composition changes in Murrah buffaloes (Bubalus bubalis) during winter season

Abstract

Present investigation was carried out to study the prevalence of bacterial pathogens in clinical and subclinical mastitis in buffaloes during winter season. A total of 118 Murrah buffaloes were screened using California mastitis Test (CMT). Milk samples were collected and analyzed for fat, protein, lactose, SNF, pH, Electrical conductivity (EC) and Somatic Cell Counts (SCC). Out of 118 milk samples, 60 samples (50.84%) were negative for CMT and 58 samples (49.15%) were detected positive for mastitis incidence. Milk pH and EC was significantly higher (p<0.05) in mastitis affected samples than the normal ones, however, protein, fat, SNF and lactose were lower (p<0.05). SCC ranged between 1.28-1.48 x 10 5 cells/ml (in normal milk samples as compared to 3.85-6.21x 10 5 cells/ ml (p<0.05) in mastitis milk samples. Out of 58 samples only 51 samples exhibited bacterial growth. The culturally examined and characterized samples revealed S. aureus (35.29%) as the predominant bacteria followed by S. agalactiae with an isolation rate of 25.49%. The incidence of coliforms bacteria was not detected in any of the sample cultured and examined. It was concluded that mastitis incidence adversely affect quality of milk by increasing the SCC, pH and EC of milk. Appropriate measures needs to be taken to prevent the incidence of S. aureus bacteria which was the major causative agent.

Implementation of a milking system and quality management for buffalo milk at an experimental station in Rio Grande do Sul, Brazil

“…76, November 26, 2018 (7) , of the Ministry of Agriculture, Livestock and Supply (MAPA), and only the State of São Paulo has specific limits for buffalo milk, set forth in Resolution SAA -03 (19) . One of the parameters for evaluating the microbiological quality of milk is SCC, which can be high in cases of mastitis, an inflammation of the mammary gland that can also bring about other changes in milk composition, such as fat, protein and lactose, leading to a reduction of these components (20) . With the exception of the months of July and September, in the other months, the results obtained for SCC were below that established by NI 76 -5.0 x 10 5 cells/mL (7) .…”

Implementação de um sistema de ordenha e manejo da qualidade do leite bubalino em uma estação experimental no Rio Grande do Sul, Brasil

“…Um dos parâmetros para avaliação da qualidade microbiológica do leite é a CCS, que pode ser elevada em casos de mastite, uma inflamação na glândula mamária que pode promover também outras alterações na composição do leite, como a gordura, proteína e lactose, levando à redução destes componentes (20) . Com exceção dos meses de julho e setembro, nos demais meses os resultados obtidos para CCS apresentaram-se abaixo do estabelecido pela IN 76 de 5.0 x10 5 cél./mL (7) .…”