Isolation and characterization of microsatellite loci for Hymenaea courbaril and transferability to Hymenaea stigonocarpa, two tropical timber species

Abstract

Hymenaea courbaril is a tropical timber species, intensely exploited and found in the Amazon, Atlantic Forest and Brazilian Cerrado biome. Nine highly polymorphic microsatellite loci were developed from a genomic library enriched for AG/TC repeats. In a total of 41 individuals, from two natural populations, seven to 13 alleles per locus were detected and expected heterozygosity ranged from 0.75 to 0.90. Seven loci were effectively transferred to Hymenaea stigonocarpa. High levels of polymorphism make the present primers useful for population genetic studies and are a powerful tool to investigate mating system, gene flow and spatial genetic structure.

Transferability and characterization of simple sequence repeat markers from Anacardium occidentale to A. humile (Anacardiaceae)

“…Similar transferability rates for microsatellite markers with high levels of polymorphism have been reported in other studies of plants such as Arachis (Bravo et al, 2006) and Hymenaea (Ciampi et al, 2008) or those from different genera such as among the species of the Poaceae family (Elazreg et al, 2011). These results demonstrate that several species, which may or may not be closely related, can be analyzed without the need for designing specific primers.…”

“…Similar results for H E and H O were found in a population of P. lentiscus L. by using specific microsatellite markers (H E ranged from 0.291 to 0.848 and H O from 0.143 to 0.762) (Albaladejo et al, 2008). The mean values for H O and H E were also close to those found with heterologous SSR in H. stigonocarpa (0.389 and 0.601, respectively) (Ciampi et al, 2008) and in 2 populations of Tabebuia roseoalba (with average H O of 0.416 and 0.490 and H E of 0.773 and 0.740) (Feres et al, 2009 A = number of alleles per locus; H E = expected heterozygosity; H O = observed heterozygosity; f = coefficient of endogamy; Q = probability of paternity exclusion; I = probability of genetic identity; QC = combined probability of paternity exclusion; IC = combined probability of genetic identity. ns = non-significant values (P > 0.00455).…”

“…The average number of alleles per locus was less than those found for Eugenia dysenterica (10.4) (Zucchi et al, 2002) and Festuca arundinacea (7.8) (Elazreg et al, 2011). The observed number of alleles per locus was closer to the values observed for Hymenaea stigonocarpa (6.43) (Ciampi et al, 2008), Dactylis glomerata (5.45), Lolium perenne (4.50) (Litrico et al, 2009) and Blighia sapida (3.7) (Ekué et al, 2009). Albaladejo et al (2008) suggested that the number of alleles per locus observed for microsatellite loci in Pistacia lentiscus (3-13 alleles) provides highly informative content for the loci in population studies.…”

“…Several studies have shown that the homology of DNA sequences in microsatellite flanking-region pairs has allowed SSR markers that have been developed for one species to be transferred to another species of the same genus or even different genera (Zucchi et al, 2002;Ciampi et al, 2008;Ekué et al, 2009;Feres et al, 2009;Litrico et al, 2009;Elazreg et al, Use of SSR markers from A. occidentale to A. humile 2011; Cristofani-Yaly et al, 2011). The success of marker transfer is inversely proportional to the evolutionary distance between the 2 species (Schlotterer et al, 1991;Moore et al, 1991;FitzSimmons et al, 1995;Rossetto, 2001).…”

Ecologia e diversidade genética de Diospyros hispida Alph. D.C. em duas áreas de cerrado no Estado de São Paulo

“…Essa característica permitiu a realização de inúmeros estudos, dentre os quais pode-se destacar os trabalhos com espécies do Cerrado: Zucchi et al (2002) de Eucalyptus spp para Eugenia dysenterica; Martins et al (2006) de Capsicum spp para Solanum lycocarpum e Ciampi et al (2008) de Hymenaea courbaril para Hymenaea stigonocarpa, entre outros.…”