Isolation and characterization of microsatellite markers for the waratah, <i>Telopea speciosissima</i> (Proteaceae)

Porter, Carolyn; Rymer, Paul D.; Rossetto, Maurizio

doi:10.1111/j.1471-8286.2006.01266.x

Cited by 3 publications

(5 citation statements)

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“…Primmer et al 1996;Steinkellner et al 1997), 'taxonomic range of marker assay' on the proportions of amplified and polymorphic markers (Table 3). Reptiles, birds, mammals, and invertebrates other than arthropods clearly fared best within Hale et al 2002;Escribano et al 2004;Jones et al 2004;Morillo et al 2004;Topinka et al 2004;Perez et al 2006;Porter et al 2006;Terui et al 2006;Salywon & Dierig 2006. §Mammals: Gemmell et al (1997; Ortega et al 2002;Williamson et al 2002;Gaur et al 2003;Dawson et al 2004;Maudet et al 2004;Gunn et al 2005.…”

Section: Distribution Of Cross-species Transfer Successmentioning

confidence: 99%

Cross‐species transfer of nuclear microsatellite markers: potential and limitations

et al. 2007

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Molecular ecologists increasingly require 'universal' genetic markers that can easily be transferred between species. The distribution of cross-species transferability of nuclear microsatellite loci is highly uneven across taxa, being greater in animals and highly variable in flowering plants. The potential for successful cross-species transfer appears highest in species with long generation times, mixed or outcrossing breeding systems, and where genome size in the target species is small compared to the source. We discuss the implications of these findings and close with an outlook on potential alternative sources of cross-species transferable markers.

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Section: Distribution Of Cross-species Transfer Successmentioning

confidence: 99%

Cross‐species transfer of nuclear microsatellite markers: potential and limitations

et al. 2007

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“…[18]. Genotyping results were checked with Microchecker [19] for evidence of scoring errors due to stuttering, large allele dropout and null alleles with ranked-based and binomial-based estimates showing no evidence of scoring error.…”

Section: Methodsmentioning

confidence: 99%

“…Seven nuclear microsatellites (nSSR) specifically developed for T. speciosissima (TS03bgt, TS04bgt, TS12bgt, TS13bgt, TS18bgt, TS23bgt and TS27bgt) were amplified across all individuals using the PCR protocols of Porter et al . [ 18 ]. Genotyping results were checked with Microchecker [ 19 ] for evidence of scoring errors due to stuttering, large allele dropout and null alleles with ranked-based and binomial-based estimates showing no evidence of scoring error.…”

Section: Methodsmentioning

confidence: 99%

Genetic structure and bio-climatic modeling support allopatric over parapatric speciation along a latitudinal gradient

et al. 2012

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BackgroundFour of the five species of Telopea (Proteaceae) are distributed in a latitudinal replacement pattern on the south-eastern Australian mainland. In similar circumstances, a simple allopatric speciation model that identifies the origins of genetic isolation within temporal geographic separation is considered as the default model. However, secondary contact between differentiated lineages can result in similar distributional patterns to those arising from a process of parapatric speciation (where gene flow between lineages remains uninterrupted during differentiation). Our aim was to use the characteristic distributional patterns in Telopea to test whether it reflected the evolutionary models of allopatric or parapatric speciation. Using a combination of genetic evidence and environmental niche modelling, we focused on three main questions: do currently described geographic borders coincide with genetic and environmental boundaries; are there hybrid zones in areas of secondary contact between closely related species; did species distributions contract during the last glacial maximum resulting in distributional gaps even where overlap and hybridisation currently occur?ResultsTotal genomic DNA was extracted from 619 individuals sampled from 36 populations representing the four species. Seven nuclear microsatellites (nSSR) and six chloroplast microsatellites (cpSSR) were amplified across all populations. Genetic structure and the signature of admixture in overlap zones was described using the Bayesian clustering methods implemented in STUCTURE and NewHybrids respectively. Relationships between chlorotypes were reconstructed as a median-joining network. Environmental niche models were produced for all species using environmental parameters from both the present day and the last glacial maximum (LGM).The nSSR loci amplified a total of 154 alleles, while data for the cpSSR loci produced a network of six chlorotypes. STRUCTURE revealed an optimum number of five clusters corresponding to the four recognised species with the additional division of T. speciosissima into populations north and south of the Shoalhaven River valley. Unexpectedly, the northern disjunct population of T. oreades grouped with T. mongaensis and was identified as a hybrid swarm by the Bayesian assignment test implemented in NewHybrids. Present day and LGM environmental niche models differed dramatically, suggesting that distributions of all species had repeatedly expanded and contracted in response to Pleistocene climatic oscillations and confirming strongly marked historical distributional gaps among taxes.ConclusionsGenetic structure and bio-climatic modeling results are more consistent with a history of allopatric speciation followed by repeated episodes of secondary contact and localised hybridisation, rather than with parapatric speciation. This study on Telopea shows that the evidence for temporal exclusion of gene flow can be found even outside obvious geographical contexts, and that it is possible to make significant progress towards ...

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“…Leaf material was collected and total genomic DNA was extracted using DNeasy ® 96 plant kits (QIAGEN ® , Hilden, Germany). Seven simple sequence repeat (SSR) loci specifically developed for T. speciosissima [ 17 ] were used in this study. Six loci (TS03bgt, TS04bgt, TS12bgt, TS18bgt, TS23bgt, TS27bgt) were dimers (CT repeats) while TS16bgt included a dimer (CT) and a tetramer (CTCA).…”

Section: Methodsmentioning

confidence: 99%

“…Six loci (TS03bgt, TS04bgt, TS12bgt, TS18bgt, TS23bgt, TS27bgt) were dimers (CT repeats) while TS16bgt included a dimer (CT) and a tetramer (CTCA). PCR conditions followed those reported by Porter et al [ 17 ]. To test genotyping accuracy PCRs were repeated for over 20% of the reactions.…”

Section: Methodsmentioning

confidence: 99%

The impact of distance and a shifting temperature gradient on genetic connectivity across a heterogeneous landscape

Rossetto

Thurlby

Offord³

et al. 2011

BMC Evol Biol

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BackgroundInter-population distance and differences in breeding times are barriers to reproduction that can contribute to genotypic differentiation between populations. Temporal changes in environmental conditions and local selective processes can further contribute to the establishment of reproductive barriers. Telopea speciosissima (Proteaceae) is an excellent subject for studying the effect of geographic, edaphic and phenological heterogeneity on genotypic differentiation because previous studies show that these factors are correlated with morphological variation. Molecular, morphological and environmental datasets were combined to characterise the relative influence of these factors on inter-population differentiation, and Bayesian analyses were used to investigate current levels of admixture between differentiated genomes.ResultsA landscape genetic approach involving molecular and morphological analyses identified three endpoints of differentiated population groups: coastal, upland and southern. The southern populations, isolated from the other populations by an edaphic barrier, show low migration and no evidence of admixture with other populations. Amongst the northern populations, coastal and upland populations are connected along a skewed altitudinal gradient by genetically intermediate populations. The strong association between temperature and flowering time in Telopea speciosissima was shown to maintain a temporally unstable reproductive barrier between coastal and upland populations.ConclusionsSubstrate-mediated allopatry appears to be responsible for long-term genetic isolation of the southern populations. However, the temperature-dependent reproductive barrier between upland and coastal populations bears the genetic signature of temporal adjustments. The extreme climatic events of the last glacial maximum are likely to have caused more complete allochronic isolation between upland and coastal populations, as well as exerting increased selective pressure upon local genomes. However, at intermediate altitudes, current climatic conditions allow for the incorporation of alleles from previously distinct genomes, generating new, intermediate genomic assemblages and possibly increasing overall adaptive potential.

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Isolation and characterization of microsatellite markers for the waratah, Telopea speciosissima (Proteaceae)

Cited by 3 publications

References 10 publications

Cross‐species transfer of nuclear microsatellite markers: potential and limitations

Cross‐species transfer of nuclear microsatellite markers: potential and limitations

Genetic structure and bio-climatic modeling support allopatric over parapatric speciation along a latitudinal gradient

The impact of distance and a shifting temperature gradient on genetic connectivity across a heterogeneous landscape

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