Isolation and Characterization of Octadecanoic Acid from The Ethyl Acetate Root Extract of Trigonella foneum graecum L. by Using Hydroponics Method

Abstract

The octadecanoic acid (OA), methyl ester was isolated and characterized from root of fenugreek (T. foenumgraecum) using ethyl acetate (EAC) in hydroponics method. The OA was characterized by TLC & HPTLC and purified by HPLC. The structure and molecular weight (298) of OA was confirmed by GC-MS. The R f value of sample (peak 4) was matched with standard. OA has strong anti-bacterial activity in B. subitlis and P. aeruginousa, where as weak activity in S. aureus and E. coli. Among the tested concentration of in vitro anti-oxidant activity of OA was maximum in 40μl and minimum in 20μl.

The effects of Piper sarmentosum aqueous extracts on zebrafish (Danio rerio) embryos and caudal fin tissue regeneration

“…*Six male (n = 6) and three females (n = 3) were used for each concentration. www.nature.com/scientificreports/ 2,4-di-tert-butylphenol, beta-asarone, asarone, n-hexadecenoic acid, phytol, or octadecanoic acid, which are abundantly present in the P. sarmentosum aqueous extracts [28][29][30][31][32][33][34] . The most abundant compound, hydrocinnamic acid, is a derivative of cinnamic acid, a compound that exhibits strong antioxidant activity due to the presence of a CH=CH-COOH (prop-2-enoic acid) moiety [35][36][37][38] .…”

“…Moreover, these antioxidant properties can help endogenous antioxidant defence systems to protect bone from osteoporosis in a rat model 27 . The exhibited antioxidant activity could be associated with hydrocinnamic acid, 2,4-di-tert-butylphenol, beta-asarone, asarone, n-hexadecenoic acid, phytol, or octadecanoic acid, which are abundantly present in the P. sarmentosum aqueous extracts 28 – 34 . The most abundant compound, hydrocinnamic acid, is a derivative of cinnamic acid, a compound that exhibits strong antioxidant activity due to the presence of a CH=CH–COOH (prop-2-enoic acid) moiety 35 – 38 .…”

Comparative Antimicrobial Activity and Bioactive Constituents of Oils from Rhizomes of Zingiber Officinale Roscoe Obtained by Different Extraction Methods

“…Antiarthritic, antiacne, antimicrobial, inhibits lipopolysaccharide-induced pro-inflammatory cytokine production (Park et al, 2014) 58. Stearic acid, methyl ester Antifungal, antimicrobial, antibacterial, antiviral, antioxidant (Sudharsan et al, 2010) 65.…”

Volatile Bioactive Compounds from Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Residing in the Leaf of Ocimum sanctum

“…Furthermore, Rahman et al (2014) also reported the antimicrobial potential of octadecanoic acid methyl ester and their related compounds isolated from leaf extracts of Jatropha curcas and Andrographis paniculata. Sudharsan et al (2011) also claimed that octadecanoic acid methyl ester isolated from Trigonella foneum-graecum L. possess potential antibacterial and antioxidant activities. Their findings revealed that the ethyl acetate extract from the plant root exhibited strong antibacterial activity against P. aeruginosa and B. subtilis whereas poor activity towards S. aureus and E. coli.…”