Isolation and characterization of pathogenic Vibrio alginolyticus from diseased postlarval abalone, Haliotis diversicolor supertexta (Lischke)

Abstract

Outbreaks of serious mortality among cultured abalone postlarvae have occurred across Southern China since July 2002. Five motile bacterial strains were isolated from diseased abalone postlarvae on tryptic soy agar supplemented with 1% NaCl (TSA1) and/ or thiosulphate citrate bile salt (TCBS) sucrose agar plates during an outbreak in August 2003 in Shanwei, Guangdong province. All isolates were characterized and identi¢ed asVibrio alginolyticus on the basis of biochemical characteristics and comparisons with those of the reference strainV. alginolyticus ATCC 17749. Strain 19 (a representative of ¢ve similar isolates) was virulent to abalone postlarvae with an LD 50 value of 1.00 Â 10 4 colony-forming units mL À1 . All abalone postlarvae exhibited the same signs as in natural outbreaks. The same bacterium could be re-isolated from abalone postlarvae after bacterial challenge using TSA1and TCBS plates. The results reveal thatV. alginolyticus is an infectious agent of abalone postlarvae.

Suppressor of cytokine signaling 2 (SOCS-2) homologue in disk abalone: Cloning, sequence characterization and expression analysis

“…Diseases and mass mortality among cultured abalone have been reported by different pathogens such as: withering syndrome in Haliotis diversicolor supertexta by Vibrio parahemolyticus [14]; disease in post-larval abalone H. diversicolor supertexta by pathogenic Vibrio alginolyticus [15]; disease amyotrophia in Nordotis discus discus by virus [16]. However, there was no report on characterization and expression of SOCS-2 gene to date in mollusk.…”

“…Most of the bacterial diseases in bivalves are reported by Gram-negative bacteria and mostly from the genus Vibrio[24]. Furthermore, several Vibrio[14,15,25] and other bacterial strains[26] were associated with mass mortality of abalone. Therefore, in bacteria challenge experiment, we have included the two Vibrio strains (V. alginolyticus, V. parahemolyticus) and one Gram-positive bacteria L. monocytogenes.…”

Investigation of several virulence properties among Vibrio alginolyticus strains isolated from diseased cultured fish in Tunisia

“…Although most studies have focused on Vibrio anguillarum as an etiological agent, other members of the genus Vibrio, such as V. alginolyticus, have also been implicated in epizootics of cultured and wild marine fishes throughout the world as well as with disease in larval and juvenile bivalve molluscs and crustaceans (Zorilla et al 2003a, Xie et al 2005, Cai et al 2006, Jayaprakash et al 2006. The knowledge of virulence properties of this emergent species could be useful for designing adequate prophylactic and antimicrobial treatments.…”

Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor

“…Bacterial infection is likely to be an important factor in this mortality (Nicolas et al 2002, Handlinger et al 2005. Different types of Vibrio infections leading to abalone death over a wide geographical area have been reported, including V. alginolyticus, V. parahaemolyticus and V. vulnificus isolated from H. diversicolor (Wang et al 1999, Liu et al 2000, Cai et al 2006, V. carchariae (V. harveyi) from H. tuberculata (Jean-Louis et al 2002, Nicolas et al 2002, and V. fluvialis and V. harveyi from H. discus hannai (Li et al 1998, Austin 2010.…”