Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest

Rajamanikyam, Maheshwari; Vadlapudi, Varahalarao; Parvathaneni, Sai Prathima; Koude, Dhevendar; Prabhakar, S.; Misra, Sunil; Amanchy, Ramars; Upadhyayula, Suryanarayana Murty

doi:10.17179/excli2017-145

Cited by 16 publications

(11 citation statements)

References 35 publications

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“…Dodecanoic acid,1,2,3-propanetriyl ester, also known as Trilaurin, presented its use in skin conditioning agents (for maintaining skin functions) as well as occlusive or viscosity increasing agents [11]. Rajamanikyam et al [12] studied the antibacterial activity of phthalic acid ester against different bacterial species such as S. epidermidis, S. aureus, P. aeruginosa, Klebsiella pneumoniae, and Bacillus subtilis. In addition, Bakkali et al [13] suggested the use of phthalic acid ester compound in skin cosmetic applications.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Fatty Acids, Polysaccharides, Amino Acids, and Minerals in Marine Macroalga Chaetomorpha crassa and Evaluation of Their Potentials in Skin Cosmetics

Kalasariya¹,

Patel²,

Yadav

et al. 2021

Molecules

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Cosmetic industries are highly committed to finding natural sources of functional active constituents preferable to safer materials to meet consumers’ demands. Marine macroalgae have diversified bioactive constituents and possess potential benefits in beauty care products. Hence, the present study was carried out to characterize the biochemical profile of marine macroalga Chaetomorpha crassa by using different techniques for revealing its cosmetic potentials. In results, the FTIR study characterized the presence of different bioactive functional groups that are responsible for many skin-beneficial compounds whereas six and fifteen different important phycocompounds were found in GCMS analysis of ethanolic and methanolic extracts, respectively. In the saccharide profile of C. crassa, a total of eight different carbohydrate derivatives were determined by the HRLCMS Q-TOF technique, which showed wide varieties of cosmetic interest. In ICP AES analysis, Si was found to be highest whereas Cu was found to be lowest among other elements. A total of twenty-one amino acids were measured by the HRLCMS-QTOF technique, which revealed the highest amount of the amino acid, Aspartic acid (1207.45 nmol/mL) and tyrosine (106.77 nmol/mL) was found to be the lowest in amount among other amino acids. Their cosmetic potentials have been studied based on previous research studies. The incorporation of seaweed-based bioactive components in cosmetics has been extensively growing due to its skin health-promoting effects.

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Section: Discussionmentioning

confidence: 99%

Characterization of Fatty Acids, Polysaccharides, Amino Acids, and Minerals in Marine Macroalga Chaetomorpha crassa and Evaluation of Their Potentials in Skin Cosmetics

Kalasariya¹,

Patel²,

Yadav

et al. 2021

Molecules

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“…11 A number of research works in the past two decades have been discussed on the isolation of DEHP from various plant samples, 12,13 algal extracts, 14 and bacterial cultures. 5,9 Certain cyanobacteria, such as Anabaena sp., Oscillatoria sp., and so on, are known to produce phthalic acid esters, but isolation of DEHP is still less evident than other phthalates like di-n-butyl phthalate and mono (2ethylhexyl) phthalate. Scientists have noticed that cyanobacteria usually release phthalate esters for surviving under stress conditions.…”

Section: Introductionmentioning

confidence: 99%

“…1 Di-(2-ethylhexyl) Phthalate (DEHP) is a conventional plasticizer used for providing flexibility to polyvinyl chloride. 2 The ester is omnipresent in nature as a synthetic pollutant from industrial and municipal sewages, posing severe health risks, including mutations and carcinomas due to DNA damage as a consequence of enhanced production of reactive oxygen species and free radicals 3,4 ; cell cycle arrest by inhibition of DNA synthesis at the S phase 5 ; impairment of liver, lungs, kidney, heart, and reproductive organs etc. 6 Moreover, serious complications related to cognitive/behavioral development have reported in children and even neonates stemming from disruption of endocrine pathways under extreme DEHP exposure.…”

Section: Introductionmentioning

confidence: 99%

Computational optimization of in vitro parameters for di‐(2‐ethylhexyl) phthalate production from Anabaena circinalis

Sarkar¹,

Andavan²

2018

J of Cellular Biochemistry

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Di-(2-ethylhexyl) phthalate (DEHP) is a ubiquitous environmental toxicant, and finds extensive commercial application as a plasticizer to reduce the rigidity of polyvinyl chloride. Besides numerous negative impacts on environment and public health, the compound exhibits acute bioactivity against microbes and has therapeutic value too. Considering this biochemical significance, searching of its new biogenic sources has become an active area of research. Here, DEHP is identified from the biomass of a toxic strain of Anabaena circinalis, which is quite unobvious, and simultaneously, the in vitro physical conditions are optimized by using a swarm-based multiparameter optimization technique. A purified fraction collected from column chromatography is subjected to gas chromatography (GC) to fetch the compound peak and then subsequent mass analysis for its identification. The mass spectrum describes the molecular weight along with the structure of DEHP with 99.9% experimental accuracy. An experimental observation table has been used to frame a fitness function using the curve fitting approach when temperature (T), light and dark period (LD), and duration of subculture cycle (DSC) are considered as the target parameters to be optimized. The optimum values obtained for T, LD, and DSC are 20°C, 14:10 hour light and dark ratio approximately, and 40 days, respectively. This experimental finding of A. circinalis FSS 124 as a novel source of DEHP and subsequent optimization using soft computing tools might be a benchmark for process optimization in biological research.

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“…Cell cycle assay was performed using modified protocol from Darzynkiewicz et al, 1999;Rajamanikyam et al, 2017 andKantapan et al, 2020 [44-46]. HeLa human cervical cancer cells (2.5 × 10 5 cells) were seeded in 6-well plates and cultured for 24 h. DMC and the available anticancer drugs (5-fluorouracil, cisplatin, and doxorubicin) were applied to HeLa cells at their IC 50 concentrations for 6, 12, 24, and 48 h. Untreated cells and cells treated with 1% (v/v) DMSO were used as the negative controls.…”

Section: Cell Cycle Assay By Flow Cytometrymentioning

confidence: 99%

Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone from Syzygium nervosum Seeds on Antiproliferative, DNA Damage, Cell Cycle Arrest, and Apoptosis in Human Cervical Cancer Cell Lines

et al. 2022

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2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC), a natural product derived from Syzygium nervosum A. Cunn. ex DC., was investigated for its inhibitory activities against various cancer cell lines. In this work, we investigated the effects of DMC and available anticervical cancer drugs (5-fluorouracil, cisplatin, and doxorubicin) on three human cervical cancer cell lines (C-33A, HeLa, and SiHa). DMC displayed antiproliferative cervical cancer activity in C-33A, HeLa, and SiHa cells, with IC50 values of 15.76 ± 1.49, 10.05 ± 0.22, and 18.31 ± 3.10 µM, respectively. DMC presented higher antiproliferative cancer activity in HeLa cells; therefore, we further investigated DMC-induced apoptosis in this cell line, including DNA damage, cell cycle arrest, and apoptosis assays. As a potential anticancer agent, DMC treatment increased DNA damage in cancer cells, observed through fluorescence inverted microscopy and a comet assay. The cell cycle assay showed an increased number of cells in the G0/G1 phase following DMC treatment. Furthermore, DMC treatment-induced apoptosis cell death was approximately three- to four-fold higher compared to the untreated group. Here, DMC represented a compound-induced apoptosis for cell death in the HeLa cervical cancer cell line. Our findings suggest that DMC, a phytochemical agent, is a potential candidate for antiproliferative cervical cancer drug development.

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Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest

Cited by 16 publications

References 35 publications

Characterization of Fatty Acids, Polysaccharides, Amino Acids, and Minerals in Marine Macroalga Chaetomorpha crassa and Evaluation of Their Potentials in Skin Cosmetics

Characterization of Fatty Acids, Polysaccharides, Amino Acids, and Minerals in Marine Macroalga Chaetomorpha crassa and Evaluation of Their Potentials in Skin Cosmetics

Computational optimization of in vitro parameters for di‐(2‐ethylhexyl) phthalate production from Anabaena circinalis

Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone from Syzygium nervosum Seeds on Antiproliferative, DNA Damage, Cell Cycle Arrest, and Apoptosis in Human Cervical Cancer Cell Lines

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