Isolation and characterization of phytosteroid and Triterpenods from leaves of abutilon crispum

Mohan, Muthugonder; Paty, S.Gana; Sudha, B.

doi:10.22376/ijpbs.8.3.p190-195

Cited by 1 publication

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So far, few information of phytochemical feature of this plant has been revealed. Thus, it was reported that β -sitosterol, lupeol, and β -amyrin are contained in the leaves of this plant [29]. In addition, genus Abutilon has been reported to contain flavonoids, phenolic acids, sterols, triterpenes, coumarins, alkaloids, lactones, megastigmanes, and iridoids [22].…”

Section: Discussionmentioning

confidence: 99%

Phosphatidylinositide 3‐Kinase Contributes to the Anti‐Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract

Hunto

Shin

Kim

et al. 2018

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Abutilon crispum L. Medik, better known as bladdermallow, is used as a traditional remedy in India, for its anti-inflammatory effect due to its high content of flavonoids. However, research about its anti-inflammatory effect at the molecular level has not been performed. In this study, we aimed to investigate the mechanism of Abutilon crispum methanol extract (Ac-ME) in inhibiting the inflammatory response by conducting several experiments including cellular and molecular assays. Ac-ME inhibited the production of nitric oxide (NO) in RAW264.7 cells during treatment of LPS and Pam3CSK4 without exhibiting cytotoxicity. Ac-ME also suppressed the mRNA expression of inducible nitric oxide (iNOS) and proinflammatory cytokines such as interleukin (IL)-1β and IL-6. Moreover, Ac-ME was shown to inhibit the NF-κB pathway, according to the luciferase reporter gene assay performed with a NF-κB-Luc construct containing NF-κB-binding promoter regions under MyD88 and TRIF overexpression conditions, and immunoblotting analysis by determining the phospho-form levels of IκBα, IKKα/β, and p85, a regulatory domain of phosphatidylinositide 3-kinase (PI3K). Finally, we observed that the level of phospho-p85 induced by the overexpression of spleen tyrosine kinase (Syk) and Src was decreased by Ac-ME at 200 μg/ml. Therefore, these results suggest that Ac-ME has an anti-inflammatory effect by targeting PI3K in the NF-κB signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%