Isolation and Characterization of Plant Growth-Promotion Diazotrophic Endophytic Bacteria Associated to Sugarcane (Saccharum officinarum L.) Grown in Paraíba, Brazil

Abstract

Sugarcane is an important Brazilian commodity, being usually cultivated in soils with low natural fertility. This study aimed to isolate diazotrophic endophytes from sugarcane tissues and evaluate the morphological and physiological characteristics of their colonies as well as their plant growth-promoting (PGP) traits in select diazotrophic endophytic bacteria. Fifty-six bacterial isolates were identified in the sugarcane tissues, and these isolates presented distinct morphological and physiological traits. A total of thirty-five bacterial isolates were biochemically evaluated. Overall, Bacillus was the dominant genus. Isolates of Methylobacterium spp. and Brevibacillus agri were present only in leaves, while Herbaspirillum HIGHLIGHTS• The diazotrophic endophytic community found in stems were different those found in leaves of sugarcane.• Endophytes with valuable biochemical traits have been identified in the sugarcane tissues.• A unique symbiotic consortium between endophytes were identified in the sugarcane tissues.

Bactérias endofíticas associadas a Paspalum vaginatum Swartz com potencial para promoção de crescimento vegetal e biocontrole

“…Pesquisas têm sido desenvolvidas em diversos tipos de culturas para identificar potenciais bactérias promotoras de crescimento vegetal, sua influência no desenvolvimento das plantas e possível uso comercial, como em milho (BATISTELA et al, 2018;AQUINO et al, 2019), cana de açúcar (ANTUNES et al, 2022;SIMÕES et al, 2019), arroz (VERMA et al, 2017PARRALES et al, 2022 e soja (ZHAO et al, 2018).…”

“…Dentre os 72 isolados bacterianos endofíticos de Paspalum vaginatum, 43 fixaram nitrogênio (59,7%). Destes, 20 isolados de folha (46,5%) e 23 de raiz (53,5%).Bactérias endofíticas diazotróficas têm sido isoladas e estudadas a partir de diversos tipos vegetais, como mandioca(LEITE et al, 2018), Brachiaria (OLIVEIRA et al, 2021, cana de açúcar(ANTUNES et al, 2022), soja (ZHAO et al, 2018 e café (ANDRADE, 2019). Liu et al (2021) avaliaram 78 bactérias endofíticas diazotróficas associadas ao Paspalum vaginatum e, assim como no presente estudo, foram identificadas bactérias diazotróficas pertencentes aos gêneros Bacillus, Stenotrophomonas e Enterobacter.…”

Whole-genome analysis revealed the growth-promoting mechanism of endophytic bacterial strain Q2H1 in potato plants

“… Cavalcante and Dobereiner (1988) first identified and isolated an endophytic nitrogen-fixing bacterium from sugarcane tissue and named it Acetobacter diazotrophicus because of its ability to oxidize ethanol to acetic acid and fix nitrogen under acidic conditions. Endophytes have been demonstrated to play crucial roles in plant disease resistance, secondary metabolite synthesis, plant growth promotion (PGP), and environmental stress resistance ( Patel and Archana, 2017 ; Afzal et al, 2019 ; Ouyabe et al, 2020 ; Lopes Antunes et al, 2022 ).…”