Isolation and characterization of polyphenols from Euphorbia heterophylla L. (Euphorbiaceae) leaves

Tostes, João Batista de Freitas; Silva, Antônio J. R. da; Kuster, Ricardo Machado

doi:10.17648/2446-4775.2019.696

Cited by 8 publications

(3 citation statements)

References 29 publications

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“…Liquid chromatography (LC) and high-performance LC (HPLC) have been widely employed to separate and isolate chemical reference materials, especially at semipreparative and preparative scales 6,7,8 . Procedures for obtaining acylglycerol-rich material have been investigated by our group, mainly involving solid phase extraction for analytical purposes or open column chromatography when a large scale was intended.…”

Section: Scale Up Of Semipreparative Liquid Chromatography In Isolati...mentioning

confidence: 99%

Scale Up of Semipreparative Liquid Chromatography in Isolating Acylglycerol-Rich Fractions

De Mattos,

Nazareth,

Muniz

et al. 2023

Rev. Foco

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Desde a inserção do biodiesel na matriz energética brasileira, é necessário garantir sua qualidade em toda a cadeia produtiva. O biodiesel tem sido produzido a partir de óleos vegetais ou gorduras animais pela reação de transesterificação, o qual ocorre por meio de reações sucessivas, levando tanto aos monoacilgliceróis (MAG) quanto aos diacilgliceróis (DAG), como compostos intermediários, e à glicerina (GL) como subproduto. A quantificação desses constituintes e dos triglicerídeos não reagidos (TAG) no controle de qualidade do biodiesel é realizada pela técnica de cromatografia gasosa (CG). Esta análise utiliza padrões destas classes de acilgliceróis, implicando em um alto custo de aquisição devido à sua elevada pureza. O presente estudo teve como objetivo desenvolver um processo de produção de frações ricas em MAG e DAG a partir da reação de glicerólise do óleo de soja, seguida de cromatografia líquida de alta eficiência, no qual foram aplicados modelos matemáticos na simulação de separações, para previsão e planejamento de transposição de escala, visando o isolamento das classes de acilgliceróis. Na primeira etapa, duas variáveis (tamanho da coluna e volume de injeção) foram aumentadas utilizando a transposição direta de escala. Os parâmetros dos picos (tempo de retenção e largura a meia altura) foram estimados a partir da deconvolução dos picos pelo software MagicPlot e verificados utilizando a expressão de momento estatístico proposta por Van Deemter et al. Assim, a segunda etapa de transposição de escala foi possível através da aplicação do modelo de momentos estatísticos proposto por Sternberg. O isolamento das frações ricas em MAG e DAG foi realizado em gradiente de fase móvel em coluna Kinetex à temperatura ambiente. As frações coletadas foram analisadas por um método de CG desenvolvido para essas matrizes. Os cromatogramas simulados concordam com os experimentais, demonstrando a aplicabilidade de aumento de escala utilizando modelos de separação de acilgliceróis. Assim, o processo de aumento de escala pela metodologia aplicada foi bem sucedido.

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Section: Scale Up Of Semipreparative Liquid Chromatography In Isolati...mentioning

confidence: 99%

Scale Up of Semipreparative Liquid Chromatography in Isolating Acylglycerol-Rich Fractions

De Mattos,

Nazareth,

Muniz

et al. 2023

Rev. Foco

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“…These metabolites were diluted in deuterated solvent, methanol (CD3OD) or dimethylsulfoxide (DMSO-d6) (Cambridge Isotope Laboratories -Massachusetts, USA). The 1 H and 13 C spectra and the homonuclear COSY and heteronuclear HSQC and HMBC correlation maps obtained were processed using the TopSpin 6.3.2 software (Bruker -Rheinstetten, Germany) and the data were compared with the literature for identification ( (Lu & Yeap Foo 1999;Xu et al 2005;Xu et al 2013;Trinh et al 2015;Guo et al 2018;Tostes et al 2019). Displacements were recorded in dimensionless δ values (ppm), considering the solvent signal as an internal reference.…”

Section: Structure Identificationmentioning

confidence: 99%

First report of phenolic compounds isolated from Inga stipularis DC. (fabaceae) leaves

Botelho

Pinheiro

Neto

et al. 2022

Natural Product Research

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Several species of the Inga genus are used by Amazonian indigenous communities to treat injuries, pain and inflammations, which is directly related to the presence of phenolic compounds in these species. Many studies have addressed the phytochemical relevance of this genus, but they are still few considering the large number of species. Therefore, this study aimed to investigate the chemical composition of Inga stipularis leaves in order to find compounds with potential pharmacological application and economic interest. The developed method allowed the isolation and identification of 8 compounds in the ethanol extract of I. stipularis: eucryphin, neoastilbin, astilbin, neoisoastilbin, isoastilbin, quercitrin, engeletin and isoengeletin. The astilbin stands out for having been isolated directly from the fractionation of the extract by SPE with high yield. This study was a pioneer for I. stipularis and revealed the potential of the species as an abundant source of compounds of pharmacological and economic interest.

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“…Since all Euphorbia parts accumulate these constituents, the stems, aerial parts, roots, fruits, seeds, flowers and in some cases the whole plant are usually investigated. The aerial part is commonly studied since it is known to contain different phenolic compounds, especially flavonoids [44][45][46]. However, isolation of these constituents is a complex procedure, because they occur in small quantities as complex mixtures of sugars with similar or related structural parent skeletal framework.…”

Section: Isolation Of Euphorbia Flavonoidsmentioning

confidence: 99%

Flavonoids from the Genus Euphorbia: Isolation, Structure, Pharmacological Activities and Structure–Activity Relationships

et al. 2021

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Plants of the genus Euphorbia are widely distributed across temperate, tropical and subtropical regions of South America, Asia and Africa with established Ayurvedic, Chinese and Malay ethnomedical records. The present review reports the isolation, occurrence, phytochemistry, biological properties, therapeutic potential and structure–activity relationship of Euphorbia flavonoids for the period covering 2000–2020, while identifying potential areas for future studies aimed at development of new therapeutic agents from these plants. The findings suggest that the extracts and isolated flavonoids possess anticancer, antiproliferative, antimalarial, antibacterial, anti-venom, anti-inflammatory, anti-hepatitis and antioxidant properties and have different mechanisms of action against cancer cells. Of the investigated species, over 80 different types of flavonoids have been isolated to date. Most of the isolated flavonoids were flavonols and comprised simple O-substitution patterns, C-methylation and prenylation. Others had a glycoside, glycosidic linkages and a carbohydrate attached at either C-3 or C-7, and were designated as d-glucose, l-rhamnose or glucorhamnose. The structure–activity relationship studies showed that methylation of the hydroxyl groups on C-3 or C-7 reduces the activities while glycosylation loses the activity and that the parent skeletal structure is essential in retaining the activity. These constituents can therefore offer potential alternative scaffolds towards development of new Euphorbia-based therapeutic agents.

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Isolation and characterization of polyphenols from Euphorbia heterophylla L. (Euphorbiaceae) leaves

Cited by 8 publications

References 29 publications

Scale Up of Semipreparative Liquid Chromatography in Isolating Acylglycerol-Rich Fractions

Scale Up of Semipreparative Liquid Chromatography in Isolating Acylglycerol-Rich Fractions

First report of phenolic compounds isolated from Inga stipularis DC. (fabaceae) leaves

Flavonoids from the Genus Euphorbia: Isolation, Structure, Pharmacological Activities and Structure–Activity Relationships

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