Isolation and Characterization of Potential Probiotic Escherichia coli Strains from Rat Faecal Samples

Kumar, Prasant; Ferzin, S.; Chintan, S.; Kumar, Gaurav

doi:10.3844/ajidsp.2009.119.124

Cited by 7 publications

(9 citation statements)

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“…Various LAB, such as L. intestinalis (10), L. plantarum (10,30), L. reuteri (3), L. helveticus and L. sakei (13) isolated from rats (10), fish (30), dogs (31), pigs (3,32), or veal calves (14), respectively, have been reported to have probiotic activity. E. coli isolated from rat gut also reportedly has probiotic activity (33).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of probiotic properties of Lactobacilli isolated from rat fecal microbiota

Jena

Trivedi

Thakore

et al. 2013

Microbiology and Immunology

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The objective of the present study was to characterize lactobacilli isolates from the feces of male Wistar rats. Various physiological features of the candidate probiotic isolates were preliminarily investigated, including tolerance to simulated gastric juice and bile salts, antimicrobial activity, antibiotic susceptibility and in vitro aggregation. Based on their morphological and biochemical characteristics, four potential probiotic isolates (CS2, CS3, CS4, and CS7) were screened. The isolates showed good tolerance to stimulated gastric juice and bile salts. CS4 and CS7 exhibited strong antibacterial activities against the pathogens tested as assessed in neutral pH culture supernatants. All lactobacilli isolates were susceptible to all the tested antibiotics, except vancomycin. Moreover, the isolate CS4 and CS7 were found to possess stronger cell surface traits such as hydrophobicity, auto-aggregation and co-aggregation capacity. In addition, CS4 and CS7 had greater b-galactosidase activities than the others. Biochemical tests and 16S rRNA gene sequencing confirmed that CS2, CS3, CS4 and CS7 are Lactobacillus intestinalis PJ2, L. sakei PJ3, L. helveticus PJ4, and L. plantarum PJ7, respectively. Based on the obtained results, L. helveticus PJ4 and L. plantarum PJ7 are ideal in vitro probiotic candidates and require further in vivo evaluation.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of probiotic properties of Lactobacilli isolated from rat fecal microbiota

Jena

Trivedi

Thakore

et al. 2013

Microbiology and Immunology

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“…at pH 3 and 60% viability at pH 2. Kumar et al [35] reported that at pH 2.0 E. coli strains 10, 20 and 16 showed higher acid tolerance and strains 3, 44, 45, 14 and 17 showed poor acid tolerance whereas at pH 3.0, all the isolates showed good acid tolerance.…”

Section: Resultsmentioning

confidence: 99%

Production, optimization and probiotic characterization of potential lactic acid bacteria producing siderophores

et al. 2017

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The aim of the study was to characterize the probiotic qualities and siderophore production of Enterococcus and Bacillus isolates for possible application for iron nutrition in human and animals strains were selectively isolated from different dairy sources and infant faecal matter. Isolates SB10, JC13 and IFM22 were found to produce maximum siderophore ranging from 65–90% at an optimum pH 7, incubation period of 96 h, agitation speed of 150 rpm and inoculum volume of 15%. SB10 and JC13 were found to show high homology with Enterococcus spp. and IFM22 with Bacillus spp., using partial 16S rRNA sequencing and biochemical characterization. All the three isolates produced hydroxymate type of siderophores under iron stressed conditions and screened for probiotic characters as per WHO guidelines. Strains have shown excellent tolerance to acid, bile salt, sodium chloride and phenol. They were non-haemolytic in nature and exhibited high hydrophobicity and autoaggregation. Our isolates proved to be potent probiotic strains due to their survival under highly acidic conditions and higher tolerance to bile salt. In addition, its colonization efficiency was proved by exhibiting high autoaggregation and hydrophobicity.

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“…Isolates which gave an inhibition zone bigger than 1 mm around the well were considered to have antimicrobial activity (Yavuzdurmaz et al, 2007;Kumar et al, 2009). The statistical analysis of the results.…”

Section: Probiotic Properties Of Isolatesmentioning

confidence: 99%

Relation between probiotic properties of isolates isolated from breast milk and infants’ stools

Sallam

Wali

Attia

et al. 2016

NFS

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Purpose Breast milk has been hypothesized to be a source of bacteria for the infant gut. This paper aims to search for probiotic bacteria among 415 isolates belonging to the Enterococcus, Lactobacillus, Streptococcus and Bifidobacterium genera recovered from breast milk of 50 lactating mothers and their respective infant stools, and then, determine whether their levels in stools vary with different modalities of breast feeding. Design/methodology/approach To prove that the isolates were probable probiotics, subtractive screening was done using three major selection criteria, namely, resistance to low pH, tolerance against bile salts and testing for their antimicrobial activity. Findings The three criteria were fulfilled by 31.7 and 31 per cent of the isolates recovered from mothers’ breast milk and infants’ stool specimens, respectively. The majority of probiotic strains, isolated from milk and infants’ stools were phenotypically identical, suggesting breast milk as their probable source. Moreover, there was a positive correlation between exclusive breast milk feeding and the number of probiotics in the infants’ stools. Originality/value Viable bacteria could be retrieved from breast milk of healthy women, not suffering from mastitis, by using different types of media and different cultivation conditions. Up to five different species belonging to the same genus could be isolated in the same specimen of whether milk or stools. Breast milk could be a source of probiotic bacteria for intestinal microflora of infants, which help improvement of infants’ gut and growth.

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Isolation and Characterization of Potential Probiotic Escherichia coli Strains from Rat Faecal Samples

Cited by 7 publications

References 0 publications

Isolation and characterization of probiotic properties of Lactobacilli isolated from rat fecal microbiota

Isolation and characterization of probiotic properties of Lactobacilli isolated from rat fecal microbiota

Production, optimization and probiotic characterization of potential lactic acid bacteria producing siderophores

Relation between probiotic properties of isolates isolated from breast milk and infants’ stools

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