Isolation and Characterization of Probiotic Bacillus subtilis MKHJ 1-1 Possessing L-Asparaginase Activity

Lim, Hyeji; Oh, Su-Jin; Yu, Sungryul; Kim, Misook

doi:10.3390/app11104466

Cited by 12 publications

(8 citation statements)

References 30 publications

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“…Except for OM02 and OM04, all other strains were found to be non-β-haemolytic, and making them suitable for usage as probiotics. Our findings are supported by research that has looked at and documented the β-haemolytic character of some strains of Bacillus cereus (24), γ-haemolysis in Bacillus velezensis GPSAK4, and Bacillus subtilis GPSAK9 (21). Furthermore, the antibiotic susceptibility study illustrated that OM03 and OM05 are susceptible to all the six antibiotics tested, as they did not harbour any of the antibiotic-resistant genes against the tested.…”

Section: Discussionsupporting

confidence: 83%

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Bacillus strains from the ovaries of Swiss albino mice (Mus musculus): deciphering of probiotic potential through an in vitro approach

Chirakkara¹,

Abraham²

2022

Biomedicine

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Introduction and Aim: The health advantages, enhanced organoleptic features, and global economic significance of probiotic-enriched food items have motivated researchers to discover novel probiotics. In light of this, a study was performed to assess the probiotic potential of five Bacillus strains isolated from the ovaries of 8-week-old mice in a previous study. Materials and Methods: The five Bacillus strains were appraised for their probiotic potentials, such as tolerance to acidic pH, bile salt, and phenol. The haemolytic behaviour, antibiotic sensitivity characteristics, and capacity to produce amylase, protease and L-asparaginase were also assessed. Results: All the isolates exhibited high survival in simulated gastric juice conditions such as pepsin containing acidic pH environments, up to 1% bile, and 0.5% phenol. Bacillus cereus OM02 and Bacillus velezensis OM03 exhibited the highest tolerance, with 50 % of survival at pH 2.5 and 1% bile salt-containing media. All the isolates were found to be amylase and protease producers and non-beta-haemolytic, however, OM02 and OM04 were beta-haemolytic. Extracts from the strain OM03 also exhibited dosage-dependent ferric-reducing antioxidant power. Except for OM04, which is resistant to ampicillin and sulphatriad, and OM01 and OM02, which are sulphatriad-resistant, all isolates were susceptible to most antibiotics. This is the first study on Bacillus species from Swiss albino mouse ovarian to evaluate probiotic potential. Conclusion: These findings suggest that Bacillus velezensis OM03, Bacillus aerius OM01, and Bacillus subtilis OM05 are suitable probiotic candidates and may be used in both animal and human formulations to improve consumer health.

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Section: Discussionsupporting

confidence: 83%

“…2). Previous research has shown that the Lasparaginase activity of the probiotic Bacillus subtilis strain derived from fermented soybeans in Korea was 1.20 U/mL (24). According to the findings of another investigation, L-asparaginase obtained from Lactobacillus sp.…”

Section: Discussionmentioning

confidence: 93%

Bacillus strains from the ovaries of Swiss albino mice (Mus musculus): deciphering of probiotic potential through an in vitro approach

Chirakkara¹,

Abraham²

2022

Biomedicine

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“…LAB belonging to the Enterococcus, Fructobacillus, Lactobacillus, Lactococcus, Leuconostoc, and Weissella genera were isolated from fruits of papaya, guava (pink and yellow varieties), custard apple, passion fruit, medlar, mulberry, khaki, and fig, and flowers of passion fruit, papaya medlar, and custard apple, in MRS medium [17]. Bacillus strains were isolated from Korean fermented soybean [58]. Lactobacilli were isolated from the oral cavity, dairy products, and feces using MRS agar at 28 • C [59].…”

Section: Discussionmentioning

confidence: 99%

An Assessment of the Lactic Acid-Producing Potential of Bacterial Strains Isolated from Food Waste

Uwamahoro

Timilsina

et al. 2022

Microbiology Research

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Lactic acid (LA) is widely used in many industries as a crucial starting material in food products, bio-based materials, and biodegradable polymers. The goals of this research were to isolate LA bacteria from food wastes, assess their potential for LA production, and study their growth characteristics. In this study, six bacterial strains were isolated from food waste and identified using 16S rRNA gene sequencing; namely, Weissella viridescens WJ39, Leuconostoc lactis YS33, Leuconostoc citreum KD42, Leuconostoc mesenteroides VN60, Macrococcus caseolyticus FCI29, and Weissella confusa RG41. W. viridescens WJ39 showed the highest potential for lactic acid production (17.56 g L−1day−1), and the lowest potential was found in L. lactis YS33 (14.09 g L−1day−1). There were significant differences (p < 0.05) in the LA production rates among Weissella spp., Leuconostoc spp., and Macrococcus spp. Moreover, dramatic differences in growth rate were observed among the six strains. W. viridescens WJ39 exhibited the highest growth rate (0.80 h−1), while M. caseolyticus FCI29 exhibited the lowest growth rate (0.57 h−1). W. viridescens WJ39 also exhibited lactic acid production (at a rate around 2 g L−1day−1) in a lab incubation experiment with food waste as a nutrient source. The draft genome of W. viridescens WJ39 with 16 contigs was constructed with an N50 of 215217 bp. The genome size was approximately 1.54 Mb, with a GC content of 41%. A hicD gene, known to catalyze the conversion of pyruvate to D-lactate, was discovered in the genome. This study illustrated the potential for the production of lactic acid from food waste with lactic acid bacteria.

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“…Disposal of effluents by industries and mismanagement of sewage waste are the two major issues that hamper the quality of drinking water. 8,9 All effluents can either be released into a stream without any treatment or only after the necessary treatment, regulations regarding sanitary conditions and pollution levels may differ. [10][11] Industries such as textile, fertilizer, chemical, agricultural etc.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization of B. subtilis from Song River Shore and their Application to Wastewater Treatment

Rakesh¹,

Kaur²,

Tiwari³

et al. 2023

J. Pure Appl. Microbiol.

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Treatment of wastewater has been a hotspot of research since ages. Emerging technologies and methodologies have been postulated to resolve the issue worldwide. Almost 97.2% of our earth is covered with water bodies, out of which 12,500 water bodies are situated in India. As per the statistics, nearly 70% water bodies are polluted in Southern Asia. Presence of emerging pollutants exacerbate the quality of flowing water. Amongst all possible ways, microbial bioremediation has been considered one of the most thriving methods to treat wastewater. This research will manifest about the isolation of B. subtilis from soil followed by its characterisation and action in treatment of wastewater which was collected from industry. From the study, it was concluded that B. subtilis holds the potential of degradation. Significant decrease in values of BOD and COD were achieved.

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Isolation and Characterization of Probiotic Bacillus subtilis MKHJ 1-1 Possessing L-Asparaginase Activity

Cited by 12 publications

References 30 publications

Bacillus strains from the ovaries of Swiss albino mice (Mus musculus): deciphering of probiotic potential through an in vitro approach

Bacillus strains from the ovaries of Swiss albino mice (Mus musculus): deciphering of probiotic potential through an in vitro approach

An Assessment of the Lactic Acid-Producing Potential of Bacterial Strains Isolated from Food Waste

Isolation, Characterization of B. subtilis from Song River Shore and their Application to Wastewater Treatment

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