Isolation and characterization of probiotic properties of Lactobacilli isolated from rat fecal microbiota

Abstract

The objective of the present study was to characterize lactobacilli isolates from the feces of male Wistar rats. Various physiological features of the candidate probiotic isolates were preliminarily investigated, including tolerance to simulated gastric juice and bile salts, antimicrobial activity, antibiotic susceptibility and in vitro aggregation. Based on their morphological and biochemical characteristics, four potential probiotic isolates (CS2, CS3, CS4, and CS7) were screened. The isolates showed good tolerance to stimulated gastric juice and bile salts. CS4 and CS7 exhibited strong antibacterial activities against the pathogens tested as assessed in neutral pH culture supernatants. All lactobacilli isolates were susceptible to all the tested antibiotics, except vancomycin. Moreover, the isolate CS4 and CS7 were found to possess stronger cell surface traits such as hydrophobicity, auto-aggregation and co-aggregation capacity. In addition, CS4 and CS7 had greater b-galactosidase activities than the others. Biochemical tests and 16S rRNA gene sequencing confirmed that CS2, CS3, CS4 and CS7 are Lactobacillus intestinalis PJ2, L. sakei PJ3, L. helveticus PJ4, and L. plantarum PJ7, respectively. Based on the obtained results, L. helveticus PJ4 and L. plantarum PJ7 are ideal in vitro probiotic candidates and require further in vivo evaluation.

Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia

“…Tolerance to low-acidic gastric and bile-rich intestinal environments is one of the essential properties required for probiotic cultures in order to function effectively in the intestines, because such conditions provide a stressful environment for bacteria [55]. The results obtained after 3 h established the possibility that the strain can survive under acidic conditions that exist in the human gut (pH 2-5), as the transit time of the food along the human gut is a maximum of 3 h [10].…”

Alteration of Intestinal Microbiota in Mice Orally Administered with Salmon Cartilage Proteoglycan, a Prophylactic Agent

“… Adlercreutzia equolifaciens has ability to produce equol [13], a nonsteroidal estrogen which has beneficial effects on the incidence of prostate cancer [14] and reduces experimental cutaneous inflammation in mice [15]. Lactobacillus intestinalis in class Bacilli , phylum Firmicutes is a potential probiotic strain [16]. Anaerovorax odorimutans is a putrescine-fermenting bacterium which changes putrescine to acetate, butyrate, and ammonia as metabolic products [17].…”

“… Parabacteroides distasonis is a bacterium which has been shown to attenuate experimental murine colitis through modulation of immunity [26]. Lactobacillus intestinalis [16], Lactobacillus johnsonii [27] and Lactobacillus reuteri [21] are a characterized by various probiotic properties. Clostridium fusiformis is a strain of gut barrier flora against pathogen, Clostridium difficile in mouse model (referred to 16S rRNA gene GenBank: AF028349).…”

“…The proportion of bacteria that provide health benefits on hosts increased in the small and large intestine upon PG administration. Lactobacilli in class Bacilli phylum Firmicutes have been reported to have probiotic properties [16,21,27]. Treatment of mice with Lactobacillus reuteri has been shown to increase the percentage of Treg cells [21].…”

Improved Gastric Acid Resistance and Adhesive Colonization of Probiotics by Mucoadhesive and Intestinal Targeted Konjac Glucomannan Microspheres

“…[10] In vitro screening of probiotics could help to select the resistant strains. [11,12] However, the process is complex and time-consuming. Alternatively, the genetic engineered probiotics can establish the resistant strains, [13] but the use of these genetically engineered products in foods are not allowed according to law regulations.…”