Isolation and characterization of silicate-solubilizing bacteria from paddy rhizosphere (<i>Oryza sativa</i>L.)

Sulizah, A.; Rahayu, Yuni Sri; Dewi, Sari Kusuma

doi:10.1088/1742-6596/1108/1/012046

Cited by 3 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A modified plate assay for the isolation of silicate solubilizing bacteria was developed by incorporating the acid base indicator dyes into the selective media with the following composition glucose 10 g/L, magnesium trisilicate 2.5 g/L and agar 20 g/L [11,14]. Observation of bacterial colonies and silicate solubilization zones formed on the conventional Bunt and Rovira media was challenging as the color of the medium was off-white.…”

Section: Developing a Modified Plate Assaymentioning

confidence: 99%

“…Silicon dioxide (1 g) was heated in muffle furnace at 500°C for 2 h. Sodium carbonate (0.2 g) was added to 0.1 g of silicon dioxide and heated at 500°C in muffle furnace for 2 h. The mixture was dissolved in boiled double distilled water and the volume was made to 1 L. Different concentrations of stock solution were transferred (0-10 mL) in respective tubes and the volume was made to 10 mL using distilled water. Absorbance values were measured at 650 nm and the standard graph was constructed [14].…”

Section: Quantification Of Silicate Solubilizationmentioning

confidence: 99%

See 1 more Smart Citation

Experimental studies on isolation and characterization of silicate solubilizing Bacillus tequilensis SKSSB09

Sukumar¹,

Swarnabala²,

Muralitharan³

2023

J App Biol Biotech

View full text Add to dashboard Cite

Silica is a beneficial mineral that regulates the growth of plants and improves crop yields. Silica is a key factor in inducing the drought mitigation property and systemic acquired resistance in plants. Screening of silicate solubilizing bacteria on conventional medium forms white colonies on off-white media with translucent zones which makes the observation challenging. The present study used a modified plate assay by incorporating 0.0025% bromocresol purple in the Bunt and Rovira media which eases the screening process. The soil sample was collected from the maize rhizosphere, seven silicate solubilizing bacteria were isolated, among which SKSSB09 showed a better result. Bacillus tequilensis showed high silicate solubilization efficiency, that is, 546.23 ± 3.4 µg/mL with a silicate solubilization zone of 7.5 ± 0.6 mm. Gas chromatography-mass spectrometry analysis of the bacterial filter extracts detected the organic acids as propanoic acid, acetic acid, butanoic acid, and glycolic acid which are crucial factors in the dissolution of insoluble silicates. SKSSB09 also possess indole acetic acid production ability and the quantitative analysis showed 61.2 ± 3.6 µg/mL. SKSSB09 possess higher antagonistic activity which showed 99% of mycelial inhibition against Fusarium oxysporum. NCBI BLAST results of 16S rRNA gene sequence identified the SKSSB09 as B. tequilensis. This work highlights the importance of silicate solubilizing B. tequilensis SKSSB09 as a plant beneficial microbial inoculant.

show abstract

Section: Developing a Modified Plate Assaymentioning

confidence: 99%

Section: Quantification Of Silicate Solubilizationmentioning

confidence: 99%