Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

Yalvaç, Mehmet Emir; Ramazanoğlu, Mustafa; Rizvanov, Albert A.; Şahın, Fikrettin; Bayrak, Ömer Faruk; Salli, Ugur; Palotás, András; Köse, Gamze Torun

doi:10.1038/tpj.2009.40

Cited by 117 publications

(117 citation statements)

References 44 publications

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“…As reported in previous studies on human stem cells (Ulloa-Montoya et al 2007;Yalvac et al 2010), Oct-4, Nanog and Sox-2 showed different relative gene expression in the cell line isolated and this is probably due to different regulatory mechanisms of these genes (Barrand and Collas 2010). Amnion cells did not express CD34, a known cell marker expressed by hematopoietic stem cells.…”

Section: Discussionmentioning

confidence: 51%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.

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Section: Discussionmentioning

confidence: 51%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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“…4 MSCs are found in bone marrow, muscle, liver, placenta, peripheral blood, synovial fluid, dental pulp, and adipose tissue. 5 They have been shown to differentiate into bone, cartilage, adipose, muscle, and nerve cells when stimulated with appropriate biological and chemical signals.…”

Section: Introductionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells enhance bone formation in orthodontically expanded maxillae in rats

Ekizer

Yalvaç

Uysal

et al. 2014

The Angle Orthodontist

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Objective: To transplant bone marrow-derived mesenchymal stem cells (MSCs) into the interpremaxillary suture after rapid maxillary expansion with the aim of increasing new bone formation in the suture. Materials and Methods: Nineteen male Wistar rats were divided into two groups (control, n 5 9; experimental, n 5 10). Both groups were subjected to expansion for 5 days, and 50 cN of force was applied to the maxillary incisors with a helical spring. Pkh67+ (green fluorescent dye)-labeled MSCs were applied to the interpremaxillary suture after force application into the interpremaxillary suture of rats. Bone formation in the sutural area was histomorphometrically evaluated, including the amount of new bone formation (mm 2 ), number of osteoblasts, number of osteoclasts, and number of vessels. Mann-Whitney U-test was used for statistical evaluation at the P , .05 level. Results: After 10 days of retention, Pkh67+ can be detected in suture mostly in the injection site under fluorescence microscope. Histomorphometric analysis revealed that a single local injection of MSCs into the midpalatal suture increased the new bone formation in the suture by increasing the number of osteoblasts and new vessel formation, compared with controls injected with phosphate-buffered saline. Conclusions: This preclinical study might provide foundations for the underlying potential clinical use of MSCs after maxillary expansion. Given the fact that MSCs are currently in use in clinical trials, this approach might be a feasible treatment strategy to accelerate new bone tissue formation in midpalatal suture and to shorten the treatment period for patients undergoing maxillary expansion reinforcement (Angle Orthod. 2015;85:394-399.)

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“…Studies have shown that extracted impacted third molars are capable of producing an optimum quantity of dental pulp tissue for the isolation of DPSCs [9,10]. Various methods have been described for isolation of stem cells from human dental pulp.…”

Section: Discussionmentioning

confidence: 99%

Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

Luisi¹,

Filho²,

Pranke³

2017

Dent Oral Craniofac Res

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Objective: The aim of this study has been the isolation and characterization of stem cells from dental pulp (DPSCs) in culture. Methods:The primary DPSCs cultures were obtained from human third molars. Immediately after extraction, the teeth were placed in Dulbecco's modified Eagle medium (DMEM) culture medium supplemented with fetal bovine serum and antibiotics. In a laminar flow, the pulp was removed from the tooth, incubated with collagenase for 2 hours and placed on a culture plate. Phenotypic characterization and cell pluripotency was performed in the fifth passage (P5). To evaluate the expression of surface markers, the cells were incubated with antibodies against CD14, CD29, CD34, CD44, CD45, CD73, CD90, CD105 and HLA-DR antigens. For induction of cell differentiation in vitro, 10 4 cells/cm 2 were plated in 12-well plates and cultivated in appropriate media for osteogenic, adipogenic and condrogencic differentiation after reaching at least 70% confluence. Results:The cells were positive above 95% for characteristic markers of the mesenchymal stem cells CD29, CD44, CD73 and CD90. In contrast, there was a low percentage of positivity (up to 1.1%) for the characteristic markers of hematopoietic cells such as CD14, CD34, CD45, CD184 and HLA-DR. Adipogenic differentiation was visualized by staining lipid vacuoles with Oil Red. Bone differentiation was visualized by staining calcium deposits with Alizarin Red. No chondrogenic differentiation was observed. Conclusions:The isolated cells were adherent to plastic, positive for the characteristic markers of mesenchymal stem cells and, therefore, an alternative source for tissue engineering studies.

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Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

Cited by 117 publications

References 44 publications

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Bone marrow mesenchymal stem cells enhance bone formation in orthodontically expanded maxillae in rats

Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

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