Isolation and characterization of subunit polypeptides from apoproteins of rat serum lipoprotein

Koga, Shunichi; Bolis, Liana; Scanu, Angelo M.

doi:10.1016/0005-2795(71)90222-4

Cited by 83 publications

(35 citation statements)

References 15 publications

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“…1). Similar results have beeni reiportedl by others (6,7,17,18,39). The specificities of the antisera were tested by double-antibody imiunoprecipitations (Tables I and II), and in the immunofluiorescenlce assay before and after absorption by appropriate apoproteins.…”

Section: D)is Cuss Ionsupporting

confidence: 75%

“…Several apoproteins have been identified in chylomicrons isolated from the intestinial lvmph of mani and rat (2)(3)(4). Amnonig these are: (a) ApoA-I (5), which is the major apoprotein (Apo) of plasmiia high density lipoprotein (6,7) and is an activator of lecithiin cholesterol acyltrainsferase (8,9); (b) ApoB (4), the major protein of plasma low density lipoprotein (LDL) and a major protein of' ApoVLDL (6, 7), which appears to be essenitial for lipoprotein foirmation iin the liver and guit (10,11); (c) arginine-rich protein (ARP or ApoE) (12) which, in rat, is found in all plasma lipoproteins (6, 7) aind levels of which appear to increase in VLDL and LDL in response to intakes of high f:at, high cholesterol diets in several animal species (13)(14)(15); and (d) ApoC, which consists of three proteinis that moduilate the activities of' lipoprotein lipase (16,17) and of lecithin cholesterol acyltransferase (9). It is suspected that intestinal VLDL have similar apoproteins, but more studies are needed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Intestinal apoproteins during fat absorption.

Schonfeld¹,

Bell²,

Alpers³

1978

J. Clin. Invest.

129

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Section: D)is Cuss Ionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Intestinal apoproteins during fat absorption.

Schonfeld¹,

Bell²,

Alpers³

1978

J. Clin. Invest.

129

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“…While recent reports have determined the apoprotein composition of serum VLDL in the rat (33,34), little information is available on the apoproteins of mesenteric chylomicrons. Since significant differences may exist between serum and lymph lipoproteins (35), it is not possible to relate our findings directly to those of others.…”

Section: Resultsmentioning

confidence: 99%

Lymph Chylomicron Formation during the Inhibition of Protein Synthesis. STUDIES OF CHYLOMICRON APOPROTEINS

Glickman¹,

Kirsch²

1973

J. Clin. Invest.

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A B S T R A C r The effect of impaired intestinal protein synthesis on chylomicron apoprotein composition was studied in mesenteric lymph fistula rats. Lymph was obtained from animals with impaired protein synthesis given intraperitoneal acetoxycycloheximide (ACH), a potent inhibitor of protein synthesis. Lymph chylomicrons were then isolated by ultracentrifugation and purified on agarose columns. Purified chylomicrons from control and ACH-treated animals were delipidated, and their apoprotein pattern was examined on sodium dodecyl sulfate (SDS) polyacrylamide gels.Because we had previously demonstrated a markedly increased lymph chylomicron size during the inhibition of protein synthesis, it was first necessary to determine whether chylomicron apoprotein composition normally varied with chylomicron size. Chylomicrons of varying sizes were prepared by differential ultracentrifugation, and their apoprotein composition was determined densitometrically on SDS polyacrylamide gels. No significant difference in apoprotein composition was found normally with varying chylomicron size.In contrast, however, chylomicrons from ACHtreated animals showed a 50% decrease in a major apoprotein band with Rr 0.67. Other

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“…It is of particular interest that its initial effect is on HDL synthesis and only subsequently on VLDL. Since rat HDL and VLDL contain subunit polypeptides which are immunologically related (36)(37)(38), it is tempting to speculate that the primary effect of clofibrate is on these particular subunit proteins. Because of this heterogeneity of the subunit proteins of the HDL and VLDL, it is possible that changes in the rate of turnover of the total proteins in a given fraction would reflect marked changes in the turnover of only some of the subunit polypeptides.…”

Section: Discussionmentioning

confidence: 99%

Effect of clofibrate on lipoprotein metabolism in hyperlipidemic rats

Segal¹,

Roheim²,

Eder³

1972

J. Clin. Invest.

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A B S T R A C T The effects of clofibrate administration were studied in rats made hyperlipidemic by the feeding of diets high in sucrose. Within 12 hr of administration of clofibrate, there was a marked decrease in the concentration of serum high density lipoproteins but no change in the concentrations of the low and very low density lipoproteins. Between 2-4 days of treatment, the concentration of the very low density lipoproteins decreased whereas that of the low density lipoproteins was unchanged. In addition, the composition of the very low density lipoproteins was altered by clofibrate administration with a decrease in triglyceride and an increase in phospholipid content.The synthesis of high density lipoproteins, as measured by the incorporation of labeled amnino acids, decreased within 12 hr of treatment. The synthesis of the very low density lipoproteins was increased during the first 2 days and then decreased slightly. The synthesis of low density lipoproteins did not change. The rate of removal of very low density lipoproteins was measured at various times from 0.5 to 8 days of treatment and was found to be elevated. It was concluded that in the hyperlipidemic sucrose-fed rat, administration of clofibrate results in a reduced level of very low density lipoproteins which may be due in part to enhancement of the rate of removal of this fraction.

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Isolation and characterization of subunit polypeptides from apoproteins of rat serum lipoprotein

Cited by 83 publications

References 15 publications

Intestinal apoproteins during fat absorption.

Intestinal apoproteins during fat absorption.

Lymph Chylomicron Formation during the Inhibition of Protein Synthesis. STUDIES OF CHYLOMICRON APOPROTEINS

Effect of clofibrate on lipoprotein metabolism in hyperlipidemic rats

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