Isolation and characterization of the trophectoderm from the Arabian camel (Camelus dromedarius)

Saadeldin, Islam M.; Swelum, Ayman A.; Elsafadi, Mona; Moumen, Abdullah F.; Alzahrani, Faisal; Mahmood, Amer; Alfayez, Musaad; Alowaimer, Abdullah N.

doi:10.1016/j.placenta.2017.06.015

Cited by 14 publications

(14 citation statements)

References 53 publications

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“…Individual dermal spheroids were collected and cultured in blank medium, and then, they were cultured in medium containing adipogenic-differentiation factors and in medium containing osteogenic-differentiation factors, as previously described [23,25].…”

Section: Cell Differentiation In Dermal Spheroidsmentioning

confidence: 99%

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Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (<em>Camelus dromedarius</em>)

Saadeldin

Swelum

Noreldin

et al. 2019

Preprint

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Elite camels often suffer from massive injuries. Thus, there is a pivotal need for a cheap and readily available regenerative medicine source. We isolated novel stem-like cells from camel skin and investigated their multipotency and resistance against various stresses. Skin samples were isolated from ears of five camels. Fibroblasts, keratinocytes, and spheroid progenitors were extracted. After separation of different cell lines by trypsinization, all cell lines were exposed to heat shock. Then, fibroblasts and dermal cyst-forming cells were examined under cryopreservation. Dermal cyst-forming cells were evaluated for resistance against osmotic pressure. The results revealed that resistance periods against trypsin were 1.5, 4, and 7 minutes for fibroblasts, keratinocytes, and spheroid progenitors, respectively. Furthermore, complete recovery of different cell lines after heat shock along with the differentiation of spheroid progenitors into neurons was observed. Fibroblasts and spheroid progenitors retained cell proliferation after cryopreservation. Dermal cyst-forming cells regained their normal structure after collapsing by osmotic pressure. The spheroid progenitors incubated in the adipogenic, osteogenic, and neurogenic media differentiated into the adipocytes, osteoblasts, and neurons, respectively. To the best of our knowledge, we isolated different unique cellular differons and stem-like cells from the camel skin and examined their multipotency for the first time.

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Section: Cell Differentiation In Dermal Spheroidsmentioning

confidence: 99%

“…Recently, the camel stem cell (SC) research has attracted some attention [23][24][25]. These studies isolated stem cells from cumulus cells [23], adipose tissue [24], and camel embryos [25]. Currently, the main targets of researchers' interest are epithelial and fibroblast differons of the skin.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (<em>Camelus dromedarius</em>)

Saadeldin

Swelum

Noreldin

et al. 2019

Preprint

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“…The ovarian cumulus cells from dromedary camels has been obtained for the first time in 2018 [41] showing that cumulus of camel can express stem cell mRNA transcript (PO5A1, KLF4, SOX2 and MYC) and are able to differentiate giving other non-ovarian follicular cells in vitro like osteoblast, neurons and adipose cells. Similarly, embryonic stem like cells have recently been isolated from dromedary camel embryo [42] which is considered highly promising for biomedical research, genetic engineering and early developmental biology. Embryonic stem cells (ESCs) and trophectoderm stem cells have been isolated from camel embryos for the first time on feeder-free conditions and showing the expression of all pluripotency genes (Sox2,Oct4, and Myc, Klf4) in the established cell lines through the conventional and real-time relative quantitative polymerase chain reactions [42].…”

Section: Stem Cellsmentioning

confidence: 99%

“…Similarly, embryonic stem like cells have recently been isolated from dromedary camel embryo [42] which is considered highly promising for biomedical research, genetic engineering and early developmental biology. Embryonic stem cells (ESCs) and trophectoderm stem cells have been isolated from camel embryos for the first time on feeder-free conditions and showing the expression of all pluripotency genes (Sox2,Oct4, and Myc, Klf4) in the established cell lines through the conventional and real-time relative quantitative polymerase chain reactions [42]. Those isolated ESCs were differentiated into neuron-like cells successfully.…”

Section: Stem Cellsmentioning

confidence: 99%

Achilles tendon rupture in camel (Camelus dromedarius): Radiographic and Ultrasonographic findings

2019

JCH

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This study describes the clinical presentation of Achilles tendon rupture and evaluates the utility of radiography and ultrasonography in the diagnosis of such disorder in dromedary camels. Seventeen camels were included in this study based on the clinical, radiographic and ultrasonographic evidence of Achilles tendon rupture. The clinical, radiographic and sonographic findings of studied camels differ according to the type, duration, and location of the tendon rupture. Complete and incomplete rupture of the Achilles tendon was precisely diagnosed in five (29.4%) and twelve (70.6%) camels respectively; ruptured deep and superficial parts of the Achilles tendon were recorded in 10 (58.8%) and 2 (11.8%) camels respectively. Clinically, the camels exhibited an acute non-weight-bearing lameness (second to fourth-grade lameness), with swelling in the tendon near the calcaneus. Radiographs revealed swelling of the soft tissues surrounding the Achilles tendon just proximal to the calcaneal tuberosity in most of the camels with the presence of avulsion fracture of the calcaneus in few cases (n=2). Ultrasonographically, the ruptured part was precisely diagnosed as swollen, oedematous, heterogeneous structure with the presence of anechoic or hypoechoic areas (core lesion). In conclusion, lateromedial radiographs and ultrasonography were helpful in diagnosis and differential diagnosis of different types of Achilles tendon rupture and subsequent clinical decision and surgical interference in dromedary camels.

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“…Because of the essential role of HSPs in thermotolerance, we and other researchers proposed the phenomenon of "anastasis" to illustrate the survival response of thermotolerant cells [36][37][38][39][40]. Anastasis is a process of plasticity, resilience, or cellular resurrection from the brink of cell death [39] and could explain the difference in the thermotolerance of cells [41].…”

Section: Introductionmentioning

confidence: 99%

Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts

Saadeldin

Swelum²,

Zakri³

et al. 2020

Animals

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This study was conducted to compare the effects of acute hyperthermia (45 °C for 4 h) on the viability, proliferation, and migratory activity through wound-healing assays of cow and sheep fibroblasts. The study examined the effects on primary cultures and first passage skin-derived fibroblasts. Relative quantification of HSP70, HSP90, P53, BAX, BCL2, and BECN1 was investigated after normalization to housekeeping genes GAPDH and beta-actin. The results revealed that cultured cow primary fibroblasts exhibited increased viability and reinitiated cell migration to close the cell monolayer scratch earlier than sheep cells. Similar patterns were observed in the first passage fibroblasts, with severe effects on sheep cells. Both cow and sheep cells exhibited decreased cell viability and failed to regain migratory activity after re-exposure of recovered heat-shocked cells. Effects of hyperthermia on sheep cells were potentiated by cell cryopreservation. The qPCR results showed that cow cells significantly increased HSP70 and HSP90 expression, which decreased the elevation of P53, and ameliorated the effects of the increased BAX/BCL2 ratio. The results provide a paradigm to compare thermotolerance among different animal species and revealed that trypsin could be an additional stress, which potentiates the effects of heat shock in in vitro experiments.

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Isolation and characterization of the trophectoderm from the Arabian camel (Camelus dromedarius)

Cited by 14 publications

References 53 publications

Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (<em>Camelus dromedarius</em>)

Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (<em>Camelus dromedarius</em>)

Achilles tendon rupture in camel (Camelus dromedarius): Radiographic and Ultrasonographic findings

Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts

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