Isolation and characterization of the high-density lipoproteins from the hemolymph and ovary of the penaeid shrimpPenaeus semisulcatus (de Haan): Apoproteins and lipids

Lubzens, Esther; Ravid, Tommer; Khayat, M.; Daube, N.; Tietz, Alisa

doi:10.1002/(sici)1097-010x(19970815)278:6<339::aid-jez1>3.0.co;2-p

Cited by 67 publications

(26 citation statements)

References 36 publications

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“…Female-specific vitellogenin is one of the HDLs, with its production being correlated with ovarian development in female crustaceans, whereas the other HDL as well as VHDL are found in both males and females. In the penaeid shrimp, P. semisulcatus, the non-sex-specific hemolymph lipoprotein, LP I, consists of one 110-kDa peptide unit, whereas the sex-specific LP II consists of 3 subunits of 200, 120, and 80 kDa [23]. Interestingly, the same subunits were also present in the lipovitellin of this shrimp.…”

Section: Vitellogeninmentioning

confidence: 81%

Mechanisms and control of vitellogenesis in crustaceans

Subramoniam

2010

Fish Sci

166

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Crustaceans produce complex yolk proteins to meet the substrate and energy requirements of embryonic development. Early electron microscopic investigations point to a biphasic yolk synthesis, first within the ovary, followed by heterosynthesis at extra-ovarian sites. Recent advances in molecular techniques have enhanced our understanding of the genetic control of yolk synthesis in crustaceans. Amino acid sequencing of crustacean vitellogenin (Vg) has enabled the elucidation of the cDNA sequence associated with it, the identification of genes, and the examination of their expression patterns in different tissues. Yolk processing in crustaeans involves cleavage of the pro-Vg at consensus sites by subtilisin-like endoproteases within the hepatopancreas, hemolymph and oocytes. The structural elucidation of crustacean yolk proteins, as well as the comparison of amino acid sequences of vitellogenins from various crustacean species, has revealed molecular phylogenetic relationships not only among them but also with other large lipid transfer lipoproteins of disparate function. The combinatorial effects of eyestalk neuropeptides and a variety of trophic hormones achieve the hormonal coordination of molting and reproduction. Biogenic amines secreted by the central nervous system may also play an integrative role by stimulating neuropeptide secretion.

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Section: Vitellogeninmentioning

confidence: 81%

Mechanisms and control of vitellogenesis in crustaceans

Subramoniam

2010

Fish Sci

166

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“…For the vitellin (the final storage form of vitellogenin in the oocyte) of two polychaetes, a lipid content of 16% and 50% has been reported (Baert et al 1984;Lee et al 2005). On the other hand, the non-sexspecific lipoproteins among the large lipid transfer proteins have also been shown to contribute to egg yolk, at least in crustacean (Lubzens et al 1997;Ravid et al 1999), insect Telfer et al 1991;Ximenes et al 2008), and chicken eggs (Steyrer et al 1990). In oocytes of some vertebrates (i.e., trout, frog, and hen) and in some insects, the incorporation of both yolk and lipoproteins is also evident from the presence of the corresponding receptors (Steyrer et al 1990;Cheon et al 2001 and references cited therein).…”

Section: Introductionmentioning

confidence: 95%

Lipoprotein mediated lipid uptake in oocytes of polychaetes (Annelida)

Schenk

Hoeger

2009

Cell Tissue Res

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The uptake of the 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-labeled sex-unspecific Nereis lipoprotein was investigated in oocytes of the nereidid polychaetes Nereis virens and Platynereis dumerilii. The fluorescence label was first observed in endocytic vesicles (<1 microm diameter), which later fused to larger vesicles (2-3 microm); these were finally incorporated into existing unlabeled yolk granules (5-6 microm). In Platynereis oocytes, the fusion of endocytic vesicles was delayed in oocytes at their final stage of development compared with those at an early stage of development. Lipoprotein double-labeled with fluorescein isothiocyanate (FITC) and DiI revealed that both the protein and the lipid moiety remained co-localized during incorporation into the yolk granules of the oocyte. No labeling of the cytoplasmic lipid droplets was observed. In N. virens, unlabeled Nereis lipoprotein was effective as a competitive inhibitor of DiI-labeled Nereis lipoprotein. Ligand blot experiments demonstrated the presence of a lipoprotein receptor with an apparent molecular mass of 120 kDa, which is different from that of the known yolk protein receptor. This indicates the presence, in the polychaete oocyte, of two distinct receptors mediating yolk protein and lipoprotein uptake, respectively. Thus, the sex-unspecific lipoprotein contributes to the lipid supply of the growing oocyte in addition to the known uptake of the yolk-protein-associated lipids. The absence of label in the cytoplasmic lipid droplets, even after prolonged incubation with labeled lipoprotein, suggests that these lipids arise either by the breakdown and resynthesis of lipoprotein-derived lipids and/or by de novo synthesis within the oocyte.

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“…In crustaceans, the lipoproteins seem to consist of a single homodimer of a w100 kDa subunit (Hall et al, 1995;Yepiz-Plascencia et al, 1995;Lubzens et al, 1997;Stieb et al, 2008), although most recently a unique lipoprotein with a apoprotein pattern similar to that of the insect lipophorins has been discovered in a freshwater crayfish (Stieb et al, 2008). A similar apoprotein pattern has been reported for the only described polychaete lipoprotein (Schenk et al, 2006).…”

Section: Introductionmentioning

confidence: 80%

“…Among the phospholipids mostly phosphatidylcholine is dominating (Katagiri, 1985;Soulages and Wells, 1994;Hall et al, 1995;Yepiz-Plascencia et al, 1995;Lubzens et al, 1997;Schenk et al, 2006), in many insects, however, phosphatidylethanolamine is the major phospholipid (Soulages and Wells, 1994). While in most arthropod lipoproteins diacylglycerols are the predominating neutral lipid species (Spaziani et al, 1986;Lee, 1991;Soulages and Wells, 1994;Hall et al, 1995;Yepiz-Plascencia et al, 1995;Lubzens et al, 1997) the predominant neutral lipid class in chelicerates is considered to be triacylglycerol (Rehn and Lee, 1994;Cunningham et al, 2007). In the tarantula Eurypelma californicum, however diacylglycerols were found to be the major neutral lipid (Haunerland and Bowers, 1987).…”

Section: Introductionmentioning

confidence: 99%