Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq

Al‐Hawash, Adnan B.; Alkooranee, Jawadayn Talib; Abbood, Hayder A.; Zhang, Jialong; Sun, Jin; Zhang, Xiaoyu; Ma, Fuying

doi:10.1016/j.btre.2017.12.006

Cited by 96 publications

(62 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was also reported that two crude oil‐degrading fungi isolated from the Rumaila oilfield, Penicillium sp. RMA1 and RMA2 could grow in media containing crude oil and further degrade (57% and 55%, respectively), illustrating that those two fungi could metabolize crude oil efficiently (Al‐Hawash et al., ).…”

Section: Resultsmentioning

confidence: 99%

Removal and biodegradation of different petroleum hydrocarbons using the filamentous fungus Aspergillus sp. RFC‐1

Al‐Hawash

Zhang

2018

MicrobiologyOpen

Self Cite

View full text Add to dashboard Cite

Petroleum pollution inevitably occurs at any stage of oil production and exerts a negative impact on the environment. Some microorganisms can degrade petroleum hydrocarbons (PHs). Polluted sludge of Rumaila oil field was use to isolate the highly efficient hydrocarbon‐degrading fungal strain. Aspergillus sp. RFC‐1 was obtained and its degradation ability for petroleum hydrocarbons was evaluated through surface adsorption, cell uptake, hydrophobicity, surface tension, biosurfactant production, and emulsification activity. In addition, the degradation mechanism was investigated. The results indicated the strain RFC‐1 showed high removal activity for PHs, including biodegradation, adsorption, and emulsifiability. On the day 7 of incubation, the removal efficiencies of crude oil, naphthalene (NAP), phenanthrene (PHE), and pyrene (PYR) reached 60.3%, 97.4%, 84.9%, and 90.7%, respectively. Biodegradation efficiencies of crude oil, NAP, PHE, and PYR were 51.8%, 84.6%, 50.3%, and 55.1%, respectively. Surface adsorption and cell absorption by live mycelial pellets followed a decreasing order: PYR ≥ PHE > NAP > crude oil. Adsorption by heat‐killed mycelial pellets increased within 40 and 10 min for crude oil and PAHs, respectively, and remained constant thereafter. Effects of cell surface hydrophobicity, surface tension, and emulsification index were discussed. Intra‐ and extracellular enzymes of strain RFC‐1 played important roles in PHs degradation. The strain RFC‐1 is a prospective strain for removing PHs from aqueous environments.

show abstract

Section: Resultsmentioning

confidence: 99%

Removal and biodegradation of different petroleum hydrocarbons using the filamentous fungus Aspergillus sp. RFC‐1

Al‐Hawash

Zhang

2018

MicrobiologyOpen

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the contrary, in the present study, the fungal community was rich of Aspergillus and Penicillium. Aspergillus and Penicillium strains have been already isolated in several polluted samples ( Chaıneau et al, 1999 ; Da Silva et al, 2003 ; Zafra et al, 2014 ; Al-Hawash et al, 2018 ). Data on soil isolation of Aspergillus niger ( Chaıneau et al, 1999 ) , Aspergillus fumigatus ( Chaıneau et al, 1999 ; Zafra et al, 2014 ), Aspergillus terreus ( Da Silva et al, 2003 ), Aspergillus flavus, Aspergillus niger, Aspergillus nomius ( Zafra et al, 2014 ), Aspergillus sydowii ( Tian et al, 2016 ), Aspergillus versicolor ( Šimonovièová et al, 2017 ) have been reported although few information deals with the here-detected species of Aspergillus (i.e., A. creber, A. jensenii, A. protuberus ).…”

Section: Discussionmentioning

confidence: 99%

Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

et al. 2018

View full text Add to dashboard Cite

Autochthonous bioaugmentation, by exploiting the indigenous microorganisms of the contaminated environment to be treated, can represent a successful bioremediation strategy. In this perspective, we have assessed by molecular methods the evolution of bacterial and fungal communities during the selective enrichment on different pollutants of a soil strongly polluted by mixtures of aliphatic and polycyclic hydrocarbons. Three consecutive enrichments were carried out on soil samples from different soil depths (0–1, 1–2, 2–3 m), and analyzed at each step by means of high-throughput sequencing of bacterial and fungal amplicons biomarkers. At the end of the enrichments, bacterial and fungal contaminants degrading strains were isolated and identified in order to (i) compare the composition of enriched communities by culture-dependent and culture-independent molecular methods and to (ii) obtain a collection of hydrocarbon degrading microorganisms potentially exploitable for soil bioremediation. Molecular results highlighted that for both bacteria and fungi the pollutant had a partial shaping effect on the enriched communities, with paraffin creating distinct enriched bacterial community from oil, and polycyclic aromatic hydrocarbons generally overlapping; interestingly neither the soil depth or the enrichment step had significant effects on the composition of the final enriched communities. Molecular analyses well-agreed with culture-dependent analyses in terms of most abundant microbial genera. A total of 95 bacterial and 94 fungal strains were isolated after selective enrichment procedure on different pollutants. On the whole, isolated bacteria where manly ascribed to Pseudomonas genus followed by Sphingobacterium, Bacillus, Stenothrophomonas, Achromobacter, and Serratia. As for fungi, Fusarium was the most abundant genus followed by Trichoderma and Aspergillus. The species comprising more isolates, such as Pseudomonas putida, Achromobacter xylosoxidans and Ochromobactrum anthropi for bacteria, Fusarium oxysporum and Fusarium solani for fungi, were also the dominant OTUs assessed in Illumina.

show abstract

“…RMA1 and RMA2 was reported to reduce the surface tension. According to the published reports, there is a direct relationship between biodegradation ability of fungi and a reduced surface tension . Therefore, Daedaleopsis sp.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

et al. 2018

View full text Add to dashboard Cite

Petroleum, as the major energy source, is indispensable from our lives. Presence of compounds resistant to degradation can pose risks for human health and environment. Basidiomycetes have been considered as powerful candidates in biodegradation of petroleum compounds via secreting ligninolytic enzymes. In this study a wood-decaying fungus was isolated by significant degradation ability that was identified as Daedaleopsis sp. by morphological and molecular identification methods. According to GC/MS studies, incubation of heavy crude oil with Daedaleopsis sp. resulted in increased amounts of C24 compounds. Degradation of asphaltene, anthracene, and dibenzofuran by the identified fungal strain was determined to evaluate its potential in biodegradation. After 14 days of incubation, Daedaleopsis sp. could degrade 93.7% and 91.2% of anthracene and dibenzofuran, respectively, in pH 5 and 40 °C in optimized medium, as revealed by GC/FID. Notably, analysis of saturates, aromatics, resins, and asphaltenes showed a reduction of 88.7% and 38% in asphaletene and aromatic fractions. Laccase, lignin peroxidase, and manganese peroxidase activities were enhanced from 51.3, 145.2, 214.5 U ml in the absence to 121.5, 231.4, and 352.5 U ml in the presence of heavy crude oil, respectively. This is the first report that Daedaleopsis sp. can degrade asphaltene and dibenzofuran. Moreover, compared to the reported results of asphaltene biodegradation, this strain was the most successful. Thus, Daedaleopsis sp. could be a promising candidate for biotransformation of heavy crude oil and biodegradation of recalcitrant toxic compounds.

show abstract

Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq

Abstract: HighlightsThe ability of indigenous fungi in degradation of crude oil was examined.Two strains exhibited a cell surface hydrophobicity higher than 70%.The two strains RMA1 and RMA2 reduced surface tension in MSM containing 1% crude oil.

Cited by 96 publications

References 25 publications

Removal and biodegradation of different petroleum hydrocarbons using the filamentous fungus Aspergillus sp. RFC‐1

Removal and biodegradation of different petroleum hydrocarbons using the filamentous fungus Aspergillus sp. RFC‐1

Molecular and Microbiological Insights on the Enrichment Procedures for the Isolation of Petroleum Degrading Bacteria and Fungi

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

Contact Info

Product

Resources

About