Isolation and characterization of two lytic phages against multidrug-resistant Salmonella and their application as a cocktail for biocontrol in foods

Pan, Hong; Shu, Mei; Li, Tianjiao; Shen, Kaisheng; Zhao, Yuan‐Yang; Liao, Ningbo; Zhong, Chunjiu; Wu, Guoping

doi:10.1016/j.lwt.2023.115184

Cited by 5 publications

(4 citation statements)

References 46 publications

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“…Phage L223 exhibited a broad range of pH (2–11) and thermal stability (≤ 70 °C). The result is consistent with other studies that also reported similar stability in Salmonella phage SWJM-01 and SWJM-02, PSDA-2, PS5, ZCSE-9 and others 3 , 31 , 35 , 36 . Such stability at a wide temperature and pH range indicated the suitability of L223 for use in food processing and utilization in foods with different pH values (meat, milk, fruits).…”

Section: Discussionsupporting

confidence: 93%

“…Consumption of Salmonella -contaminated foods leads to salmonellosis, an acute infection characterized by symptoms such as fever, nausea, vomiting, and abdominal pain 2 . Non-typhoidal salmonellosis (NTS) accounts for approximately 90 million cases of food poisoning and 155,000 deaths worldwide annually, representing a serious threat to human health 3 , 4 . In the European Union alone, Salmonella is the most frequently isolated pathogen in foodborne outbreaks, contributing to nearly 91,000 cases of salmonellosis annually 5 .…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic characterization and genomic analysis of a Salmonella phage L223 for biocontrol of Salmonella spp. in poultry

Khan,

Islam,

Barua

et al. 2024

Sci Rep

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The escalating incidence of foodborne salmonellosis poses a significant global threat to food safety and public health. As antibiotic resistance in Salmonella continues to rise, there is growing interest in bacteriophages as potential alternatives. In this study, we isolated, characterized, and evaluated the biocontrol efficacy of lytic phage L223 in chicken meat. Phage L223 demonstrated robust stability across a broad range of temperatures (20–70 °C) and pH levels (2–11) and exhibited a restricted host range targeting Salmonella spp., notably Salmonella Typhimurium and Salmonella Enteritidis. Characterization of L223 revealed a short latent period of 30 min and a substantial burst size of 515 PFU/cell. Genomic analysis classified L223 within the Caudoviricetes class, Guernseyvirinae subfamily and Jerseyvirus genus, with a dsDNA genome size of 44,321 bp and 47.9% GC content, featuring 72 coding sequences devoid of antimicrobial resistance, virulence factors, toxins, and tRNA genes. Application of L223 significantly (p < 0.005) reduced Salmonella Typhimurium ATCC 14,028 counts by 1.24, 2.17, and 1.55 log CFU/piece after 2, 4, and 6 h of incubation, respectively, in experimentally contaminated chicken breast samples. These findings highlight the potential of Salmonella phage L223 as a promising biocontrol agent for mitigating Salmonella contamination in food products, emphasizing its relevance for enhancing food safety protocols.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Phenotypic characterization and genomic analysis of a Salmonella phage L223 for biocontrol of Salmonella spp. in poultry

Khan,

Islam,

Barua

et al. 2024

Sci Rep

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“…In addition, as in Pan et al. ( 2023 ) study, studies are being carried out that Salmonella phages can be used as biocontrol agents on multidrug‐resistant bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…To control Salmonella, many Salmonella phage studies are still ongoing (Ge et al, 2022;Khan & Rahman, 2022). In addition, as in Pan et al (2023) study, studies are being carried out that Salmonella phages can be used as biocontrol agents on multidrug-resistant bacteria.…”

mentioning

confidence: 99%

Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control

Unverdi,

Erol,

Kaskatepe

et al. 2024

Food Science & Nutrition

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Salmonella is a bacterium associated with food contaminated by various animals, primarily poultry. Interest and research on bacteriophages are increasing because they can be used as an alternative against increasing antibiotic resistance. In our study, eight Salmonella‐specific lytic bacteriophages were isolated from chicken feces. Two of the isolated phages (AUFM_Sc1 and AUFM_Sc3) were chosen for their characterization due to their broader host range. Based on morphological and genomic analysis, AUFM_Sc1 was identified to be close to similar Enterobacteria spp. CC31 (Myoviridae) and AUFM_Sc3 was identified to be close to Salmonella phage vB_Sen_I1 (Demerecviridae (formerly Siphoviridae)). Although these phages have shown promise for use in phage therapy applications for chickens, further studies are needed on their suitability. When a cocktail of these phages (AUFM_Sc1 + AUFM_Sc3) and nisin combination was applied on chicken breast meat, it was determined that it was effective against Salmonella contamination and while a good inhibitory effect was observed on the food, especially during the first 48 h, the effect decreased later, but the bacterial concentration was still low compared to the control group. Therefore, it is considered that the combination of AUFM_Sc1 + AUFM_Sc3 + nisin can be used as a food preservative against Salmonella.

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Characterization and efficacy of Salmonella phage cocktail PHA46 in the control of Salmonella Newport and Typhimurium internalized into cherry tomatoes

Arista-Regalado,

Viera-Segura,

de Oca

et al. 2024

International Journal of Food Microbiology

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Isolation and characterization of two lytic phages against multidrug-resistant Salmonella and their application as a cocktail for biocontrol in foods

Cited by 5 publications

References 46 publications

Phenotypic characterization and genomic analysis of a Salmonella phage L223 for biocontrol of Salmonella spp. in poultry

Phenotypic characterization and genomic analysis of a Salmonella phage L223 for biocontrol of Salmonella spp. in poultry

Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control

Characterization and efficacy of Salmonella phage cocktail PHA46 in the control of Salmonella Newport and Typhimurium internalized into cherry tomatoes

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