Isolation and characterization ofAnaplasma marginale DNA

Bear, Phyllis D.; Philpott, Matthew

doi:10.1007/bf01577268

Cited by 5 publications

(7 citation statements)

References 12 publications

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“…The genome size of A . marginale we report is quite close to the 1500-2100 kbp reported for several other rickettsia1 agents (Frutos et al, 1989;Kingsbury, 1969), but is much larger than the previously reported 297-340 kbp genome size (Bear & Philpott, 1987).…”

Section: Resultssupporting

confidence: 85%

“…The G + C content of 56 mol YO is slightly higher than previously reported values of 49.9-50.7 and 51 mol% (Nomore, 1975; Weiss & Moulder, 1984). However, it is significantly higher than the previously reported value of 33 mol YO, determined by Bear & Philpott (1987) when calculating the genome size of A . marginale by renaturation kinetics.…”

Section: Resultscontrasting

confidence: 75%

“…This may in part account for the difference in genome size of A . marginale reported here, 1200-1260 kbp, and that previously reported using renaturation kinetics, 297-340 kbp (Bear & Philpott, 1987). Genome sizes determined by renaturation kinetics can also yield erroneous results if the DNA is not pure or if numerous multiple-copy genes are present in the genome (Bove, 1984;Pyle et al, 1988;Razin, 1985).…”

Section: Resultsmentioning

confidence: 51%

“…There is a discrepancy in the literature 0001-7930 0 1993 SGM concerning the mol% G + C content of the organism (Bear & Philpott, 1987;Normore, 1975;Weiss & Moulder, 1984), and the previously reported genome size of A . marginale (Bear & Philpott, 1987) is significantly smaller than values reported for other ricksettial agents (Frutos et al, 1989;Kingsbury, 1969).…”

Section: Introductionmentioning

confidence: 92%

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"Analysis of the Anaplasma mavginale genome by pulsed-field electrophoresis"

Alleman

Kamper²,

Viseshakul³

et al. 1993

Journal of General Microbiology

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~~Anaplasma marginale is a rickettsia1 parasite of bovine erythrocytes causing world-wide economic losses in livestock production. Despite its importance, little is known about this rickettsia at a molecular level because it has not been cultured in zlitro, and there is no small-animal model. Although several genes have been cloned and sequenced, the gross genome structure of the organism has not yet been well characterized. We separated intact bovine erythrocytes from leucocytes, and determined the genome size of A. marginale by use of restriction endonuclease cleavage and pulsed-field gel electrophoresis (PFGE). A value of 56 mol% G + C was obtained for this genome by spectral analysis. Undigested A. marginale DNA failed to migrate under several different electrophoretic conditions, indicating a circular genome. Digestions of intact A. marginale DNA were performed using restriction endonucleases NotI, SfiI and Pad. Complete digestion with SfiI resulted in 12 distinct bands ranging in size from 14 to 170 kbp. Total size determined by addition of Sfl-digested fragments was approximately 1200 kbp. P a d cleaved the A. marginale genome from three different isolates into just three fragments, of 598, 557 and 97 kbp. Incomplete digestion produced a band measuring 1250 kbp. These results indicate that A. marginale has a circular genome between 1200 and 1260 kbp, with a G + C content of 56 mol%.

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Section: Resultssupporting

confidence: 85%

Section: Resultscontrasting

confidence: 75%

Section: Resultsmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 92%

See 2 more Smart Citations

"Analysis of the Anaplasma mavginale genome by pulsed-field electrophoresis"

Alleman

Kamper²,

Viseshakul³

et al. 1993

Journal of General Microbiology

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“…The 50 control midgut tissues were uniformly negative by histologic and hybridization techniques (Fig. 4 A-C (14). However, the sensitivity exceeds that obtainable by examination of stained blood films and thus has promise in identification of chronically infected cattle.…”

Section: Resultsmentioning

confidence: 99%

Detection of Anaplasma-marginale-infected tick vectors by using a cloned DNA probe.

Goff

Barbet

Stiller

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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Anaplasmosis is the most widely distribqted of several important tick-borne diseases that constrain cttle production throughout much of the world. Evaluation of the effectiveness of disease control strategies that integrate vaccination with tick control requires the ability to monitor tick and cattle infection rates. To detect Anaplasma marginate in ticks and bovine erythrocytes, a 2-kilobase DNA fragment from a cloned A. marginale gene coding for a surface protein having a Mr of 105,000 was prepared and evaluated as a probe. The probe was species specific and detected A. marginale DNA derived from infected bovine erythrocytes and adult Dermacentor ticks infected either as nymphs or adults. Tick infection was confimed by microscopy and test feeding on a susceptible calf. The sensitivity of the probe is suitable for detecting infected ticks in experimental and field epizootiology studies.

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Development of a recombinant Anaplasma marginale DNA probe

Aboytes-Torres

Buening

1990

Veterinary Microbiology

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Isolation and characterization ofAnaplasma marginale DNA

Cited by 5 publications

References 12 publications

"Analysis of the Anaplasma mavginale genome by pulsed-field electrophoresis"

"Analysis of the Anaplasma mavginale genome by pulsed-field electrophoresis"

Detection of Anaplasma-marginale-infected tick vectors by using a cloned DNA probe.

Development of a recombinant Anaplasma marginale DNA probe

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