Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae

Abstract

Fucoidans are matrix polysaccharides from marine brown algae, consisting of an α-l-fucose backbone substituted by sulfate-ester groups and masked with ramifications containing other monosaccharide residues. In spite of their interest as biologically active compounds in a number of homologous and heterologous systems, no convenient sources with fucanase activity are available yet for the degradation of the fucalean algae. We here report on the isolation, characterization, and culture conditions of a bacterial strain capable of degrading various brown algal fucoidans. This bacterium, a member of the family Flavobacteriaceae, was shown to secrete fucoidan endo-hydrolase activity. An extracellular enzyme preparation was used to degrade the fucoidan from the brown alga Pelvetia canaliculata. End products included a tetrasaccharide and a hexasaccharide made of the repetition of disaccharidic units consisting of α-1→3-l-fucopyranose-2-sulfate-α-1→4-l-fucopyranose-2,3-disulfate, with the 3-linked residues at the nonreducing end.

Utilization of kelp-derived carbon sources by kelp surface-associated bacteria

“…Chesters & Bull 1963, Uchida 1995, Sakai et al 2002, Descamps et al 2006. In these studies, the individual strains and their physiology were the focus rather than the ecological importance of the bacteria on kelp surfaces or in kelp forest ecosystems.…”

Microbial Fucoidan Degradation byLuteolibacter algaeH18 with Deacetylation

“…Some fucoidan-degrading microorganisms have been isolated for this purpose. Kloareg et al isolated Flavobacteriaceae SW5 strain 5) and purified an enzyme specific for the degradation of the fucoidan of Pelvetia canaliculata from the culture supernatant.…”

Microorganisms living on macroalgae: diversity, interactions, and biotechnological applications

“…Only one fucanolytic gene has been cloned to date: the fucoidanase fncA from Mariniflexile fucanivorans SW5 ). This marine flavobacterium was isolated from a water-treatment facility that recycles the effluent from an algal alginate extraction plant (Barbeyron et al 2008;Descamps et al 2006). FcnA encompasses an N-terminal catalytic module (~400 residues), three immunoglobulin-like modules, and a PorSS secretion module.…”