Isolation and evaluation of Bacillus velezensis ZW-10 as a potential biological control agent against Magnaporthe oryzae

Abstract

Rice blast is one of the most destructive diseases of rice and is caused by the infection with rice blast fungus, Magnaporthe oryzae. In the present study, we obtained 12 bacterial isolates from Sichuan basin neutral purplish soil. Among them, the strain named ZW-10 showed significant activity against M. oryzae. Morphological and biochemical studies, along with 16S rDNA sequencing analysis, proved that the strain belongs to Bacillus velezensis. The cell-free culture filtrate of ZW-10 inhibited the growth of M. oryzae, suggesting the presence of active enzymes and secondary metabolites. The experimental results showed that ZW-10 produced cellulase, protease, and peroxidase. The temperature experiment indicated that the secondary metabolisms of ZW-10 remained active at high temperature. In addition, this strain had good acidity resistance. As shown in leaf experiments in vitro as well as field trials, the liquid culture and the cell-free culture filtrate of ZW-10 had significant inhibitory effects on rice blast. In conclusion, ZW-10 can be used as a potential biological agent in the control of rice blast.

The antagonistic mechanism of Bacillus velezensis ZW10 against rice blast disease: Evaluation of ZW10 as a potential biopesticide

“…It was found that the mycelia treated with 1% CFB showed obvious red fluorescence, especially the deformed mycelia and vacuolar area. In the previous experiment, we found that ZW10 could produce protease, cellulase and chitinase [22]. Therefore, the loss of mycelial permeability caused by 1% CFB treatment of M. oryzae may be related to this.…”

“…Up to now, there are only a few reports on the control of rice blast by B. velezensis. Previous studies characterized the physical and chemical properties of B. velezensis ZW10 and elucidated its antagonistic activity against M. oryzae [22]. In this paper, we aimed to explore the inhibitory mechanism of B. velezensis ZW10 and the host defense response against M. oryzae, and to evaluate the potential of ZW10 as a biological pesticide.…”

Isolation and Characterization of Bacillus velezensis Strain P2-1 for Biocontrol of Apple Postharvest Decay Caused by Botryosphaeria dothidea

“…Several strains of B. velezensis have also been reported to act as biocontrol agents, preventing plant diseases and promoting plant growth. For instance, B. velezensis strain ZW-10 exhibited activity against the Magnaporthe oryzae , via secondary metabolites ( Chen et al, 2020 ). It has been reported that B. velezensis strain 83 from the mango tree phyllosphere is capable of controlling anthracnose and promoting plant growth ( Balderas-Ruíz et al, 2020 ).…”

Bacillus Cyclic Lipopeptides Iturin and Fengycin Control Rice Blast Caused by Pyricularia oryzae in Potting and Acid Sulfate Soils by Direct Antagonism and Induced Systemic Resistance

“…As a result, chemical fungicides are gradually becoming inoperative. Therefore, the use of bacterial biocontrol agents to control rice blast is gaining attention [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ].…”