Isolation and evaluation of strong endogenous promoters for the heterologous expression of proteins in Pichia pastoris

Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii

“…Also, the promoter of ATP synthase 2 (P ATP2 ) is a promising candidate which was recently identified as a strong promoter. 27 The two promoters with the lowest fluorescence, but still significantly different from the parental strain (Figure 2C), is the Cytochrome C oxidase 6 (P COX6 ) and Yeast nucleoside diphosphate kinase 1 (P YNK1 ). Both of these promoters might not be fully active under the conditions of the experiments performed here: COX6 in S. cerevisiae is known to be repressed in a glucose medium, 28 which likely explains the low activity of the orthologue promoter in K. phaff ii in the tested glucose-rich conditions, and transcription of YNK1 is found to be associated with oxidative and DNA damage stress.…”

“…Since FBA1 is constitutively expressed in K. phaffii , P FBA1 represents a promising new promoter for the K. phaffii molecular toolbox (Figure C). Also, the promoter of ATP synthase 2 (P ATP2 ) is a promising candidate which was recently identified as a strong promoter . The two promoters with the lowest fluorescence, but still significantly different from the parental strain (Figure C), is the Cytochrome C oxidase 6 (P COX6 ) and Yeast nucleoside diphosphate kinase 1 (P YNK1 ).…”

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

“…In addition to the above modifications of targets or transcription factors involved in the regulation of P AOX1 , a large number of novel promoters suitable for the P. pastoris expression system has also been explored [48][49][50], with the recently identified promoters listed in Table 1. Among these, the promoter P ADH2 is naturally present in P. pastoris to regulate the expression of the alcohol dehydrogenase gene ADH2, which is involved in the conversion of ethanol to formaldehyde.…”