Isolation and expression analysis of salt induced genes from contrasting grapevine (Vitis vinifera L.) cultivars

Daldoul, Samia; Guillaumie, Sabine; Reustle, Götz M.; Krczal, Gabi; Ghorbel, Abdelwahed; Delrot, Serge; Mliki, Ahmed; Hofer, Michaël

doi:10.1016/j.plantsci.2010.07.017

Cited by 39 publications

(38 citation statements)

References 49 publications

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“…Differential expression of a putative alkaline a-galactosidase/SIP cDNA under different stress conditions Previous screening of a leaf subtractive cDNA library from the salt tolerant grapevine cultivar Razegui led to the identification of a cDNA fragment of 710 bp belonging to a putative alkaline alpha-galactosidase gene also referred to as seed imbibition protein (Vv-a-gal/SIP) whose corresponding mRNA was significantly induced by a moderate salt stress treatment in a genotype dependent manner (Daldoul et al 2010). We have extensive Northern analyses on independent salt stress experiments conducted in three different years using this cDNA clone and thus confirmed the genotype specific expression pattern when equal amounts of leaf derived total RNA from Razegui were compared to total RNA derived from the salt sensitive cultivar Syrah.…”

Section: Characterisation Of Plant Materialsmentioning

confidence: 99%

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Molecular cloning and characterisation of a cDNA encoding a putative alkaline alpha-galactosidase from grapevine (Vitis vinifera L.) that is differentially expressed under osmotic stress

et al. 2011

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The expression of a gene originally identified by means of a salt stress-associated partial cDNA showing a genotype dependent response was studied upon exposure to salt and drought stress and during seed development of grapevines. The complete open reading frame has been cloned and the corresponding untranslated regions have been characterized by 5 0 -and 3 0 -RACE leading to a refinement and correction of the predicted gene model in the recent 129 Vitis genome assembly. The open reading frame of the single copy gene designated Vv-a-gal/SIP encodes for a polypeptide of 774 amino acids with a calculated molecular mass of 84.9 kDa. Protein sequence comparisons suggest that the gene belongs to a growing family of plant-specific alkaline a-galactosidases. 5 0 -RACE identified a not yet documented transcript species derived from the same gene locus which does not lead to the formation of a full-length transcript. The ''aberrant'' transcript harbours an alternative 5 0 -UTR and originates from an internal transcriptional start site 1.4 kb downstream of the predominant transcriptional start site. Levels of transcripts containing the different 5 0 -UTRs were compared under salt stress conditions.

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Section: Characterisation Of Plant Materialsmentioning

confidence: 99%

“…Razegui which was significantly up-regulated following a salt stress treatment (Daldoul et al 2010). The cDNA encodes protein, which belongs to a class of poorly characterised seed imbibition proteins (SIP) with putative alkaline a-galactosidase activity whose role in abiotic stress response remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterisation of a cDNA encoding a putative alkaline alpha-galactosidase from grapevine (Vitis vinifera L.) that is differentially expressed under osmotic stress

et al. 2011

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“…Within grapevine cultivars, several studies were achieved in order to identify proteins and genes involved in salt tolerance (Cramer et al, 2007;Vincent et al, 2007;Daldoul et al, 2010;Jellouli et al, 2010). Cramer et al (2007) revealed through microarray transcript profiling, quantitative reverse-transcription polymerase chain reaction (RT-PCR), and metabolite profiling that the RD22 gene was up-regulated in Vitis vinifera L. 'Cabernet Sauvignon' thoroughly subjected to gradually applied and long-term (16 days) water-deficit stress and equivalent salinity stress.…”

Section: Introductionmentioning

confidence: 99%

“…Cramer et al (2007) revealed through microarray transcript profiling, quantitative reverse-transcription polymerase chain reaction (RT-PCR), and metabolite profiling that the RD22 gene was up-regulated in Vitis vinifera L. 'Cabernet Sauvignon' thoroughly subjected to gradually applied and long-term (16 days) water-deficit stress and equivalent salinity stress. Using a combined approach of suppression subtractive hybridization and microarray, Daldoul et al (2010) identified the RD22 gene among 7 cDNA clones that were up-regulated by salt stress in a tolerant variety (Razegui). Furthermore, in wild grapevines (Vitis vinifera L. subsp.…”

Section: Introductionmentioning

confidence: 99%

Physiological responses of transgenic tobacco plants expressing the dehydration-responsive RD22 gene of Vitis vinifera to salt stress

JAMOUSSI¹,

El-Abbassi²,

Jouira³

et al. 2014

Turk J Bot

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Salinity is one of the most common environmental stresses affecting grapevine productivity. In order to analyze the contribution of the dehydration-responsive RD22 gene of Vitis vinifera L. (VvRD22) to salt tolerance improvement, Agrobacteriummediated transformation in the tobacco model plant (Nicotiana benthamiana) was carried out and transgenic lines were subjected to in vitro and ex vitro salt treatments for physiological response evaluation. Under in vitro salt stress, the transgenic lines exhibited higher seed germination and growth than the wild type (WT). The ex vitro assays after salt treatment showed better growth and higher chlorophyll content in VvRD22-expressing plants than in the WT. Ion analysis revealed less increase in the levels of sodium (Na + ) in leaves and chloride (Cl -) in all organs within the transgenic lines compared to the WT. Interestingly, stabilized calcium (Ca 2+ ) contents were registered in the leaves of transgenic lines. Moreover, the occurrence of an osmotic adjustment based on an overproduction of total soluble sugars was observed within the transgenic lines. These physiological responses suggest that the protective effect of VvRD22 transgenic expression is enabling other physiological mechanisms to function and subsequently contributing to the ability to cope with salt stress. The VvRD22 transgenic expression would be useful in engineering salt stress-tolerant grapevines.

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“…Thus understanding the molecular genetic basis of the environmental stress responses would be crucial towards improving grapevines production. We have recently identified a novel alkaline alpha galactosidase (Vv-α-gal-SIP) from the salt tolerant Tunisian cultivar Razegui as differentially expressed upon salt stress [3]. α-Gal is widely distributed in plant species and there are two distinct types of α-Gals which degrade oligosaccharides during seed germination and differing in their optimal pH of catalysis.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and in silico analysis of an alkaline α-galactosidase gene (Vv-α-gal/SIP) in grapevines (Vitis vinifera. L)

Daldoul¹,

Hanana²,

Mliki³

2012

Turk J Bioch

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Aim: After identifying and isolating a grapevine alkaline α-galactosidase gene and before initializing functional genomic studies, we judged necessary to acquire minimal knowledge about this enzyme that catalyzes galactose release in the more neutral to alkaline cytoplasm. Our goal was to search and identify structural motives and domains that could provide knowledge and information about protein activity and functionality. Methods: We realized a bioinformatics analysis using several tools based on the homology search, promoter analysis, in order to determine basic characteristics of this protein that could guide us in the functional characterization works. As with several bioinformatics approaches, it is the combination of tools and data resources which provides the best results. Results: We discovered a new alkaline α-gal gene by using Bioinformatics tools. Vv-α-gal/ SIP gene is made of 13 exons and measures 2.9 kb, it encodes a protein of 774 amino acids with a predicted molecular mass of 85 kDa. The enzyme contains several putative abiotic stress regulatory elements. Conclusion: This study reports the first bioinformatic characterization of the Vv-α-gal-SIP gene. We investigated the possibility of predicting the function of Vv-α-gal-SIP only by its sequence. Several regulatory elements were predicted and described to be involved in abiotic stress signaling. The discovery of new alkaline α-Gals may contribute to important technological applications of α-Gals.

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Isolation and expression analysis of salt induced genes from contrasting grapevine (Vitis vinifera L.) cultivars

Cited by 39 publications

References 49 publications

Molecular cloning and characterisation of a cDNA encoding a putative alkaline alpha-galactosidase from grapevine (Vitis vinifera L.) that is differentially expressed under osmotic stress

Molecular cloning and characterisation of a cDNA encoding a putative alkaline alpha-galactosidase from grapevine (Vitis vinifera L.) that is differentially expressed under osmotic stress

Physiological responses of transgenic tobacco plants expressing the dehydration-responsive RD22 gene of Vitis vinifera to salt stress

Molecular characterization and in silico analysis of an alkaline α-galactosidase gene (Vv-α-gal/SIP) in grapevines (Vitis vinifera. L)

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