Isolation and expression analysis of a novel pathogenesis-related protein 10 gene from Chinese wild Vitis pseudoreticulata induced by Uncinula necator

Xu, Yan; Yu, Hao; He, Mingyue; Yang, Yazhou; Wang, Yuejin

doi:10.2478/s11756-010-0056-0

Cited by 13 publications

(12 citation statements)

References 23 publications

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“…The Ypr6 (proteinase inhibitor), Ypr12 (plant defensin), Ypr13 (plant thionin), and Ypr14 (lipid transfer protein) antimicrobial peptides are usually cysteine-rich peptides with broad-spectrum antifungal activities ( Loon et al, 2006 ; Nawrot et al, 2013 ). Many PR10 proteins and PR4 protrins have been shown to have RNase activity ( Xu et al, 2010 ; Wang et al, 2011 ; Yang et al, 2018 ). The recombinant Theobroma cacao PR-10 protein has antifungal activities against the basidiomycetous pathogen Moniliophthora perniciosa and the yeast Saccharomyces cerevisiae , while also exhibiting in vitro and in vivo RNase activities ( Pungartnik et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

Wang

Tang

et al. 2021

Front. Plant Sci.

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Pathogenesis-related proteins (PRs) are a class of proteins that accumulate in response to biotic and abiotic stresses to protect plants from damage. In this study, a gene encoding a PR-like protein (PnPR-like) was isolated from Panax notoginseng, which is used in traditional Chinese herbal medicines. An analysis of gene expression in P. notoginseng indicated that PnPR-like was responsive to an infection by the root rot pathogen Fusarium solani. The expression of this gene was induced by several signaling molecules, including methyl jasmonate, ethephon, hydrogen peroxide, and salicylic acid. The PnPR-like-GFP fusion gene was transiently expressed in onion (Allium cepa) epidermal cells, which revealed that PnPR-like is a cytoplasmic protein. The purified recombinant PnPR-like protein expressed in Escherichia coli had antifungal effects on F. solani and Colletotrichum gloeosporioides as well as inhibited the spore germination of F. solani. Additionally, the in vitro ribonuclease (RNase) activity of the recombinant PnPR-like protein was revealed. The PnPR-like gene was inserted into tobacco (Nicotiana tabacum) to verify its function. The gene was stably expressed in T2 transgenic tobacco plants, which exhibited more RNase activity and greater disease resistance than the wild-type tobacco. Moreover, the transient expression of hairpin RNA targeting PnPR-like in P. notoginseng leaves increased the susceptibility to F. solani and decreased the PnPR-like expression level. In conclusion, the cytoplasmic protein PnPR-like, which has RNase activity, is involved in the P. notoginseng defense response to F. solani.

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Section: Discussionmentioning

confidence: 99%

A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

Wang

Tang

et al. 2021

Front. Plant Sci.

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“…These families have been numbered in sequence of discovery ( Sels et al, 2008 ; Sinha et al, 2014 ). Usually, PRs possess enzymatic functions – so, for example, PR-2 is a 1,3-β-glucanase ( Kauffmann et al, 1987 ), PR-7 has endoproteinase activity, PR-9 is a peroxidase, and PR-10 is a ribonuclease ( Lagrimini et al, 1987 ; Vera and Conejero, 1988 ; Park et al, 2004 ; Xu et al, 2010 ). Moreover, a number of them, e.g., PR-1 and PR-6, have been identified as having antifungal and proteinase inhibitory properties ( Green and Ryan, 1972 ; Niderman et al, 1995 ).…”

Section: Introductionmentioning

confidence: 99%

The Novel Gene VpPR4-1 from Vitis pseudoreticulata Increases Powdery Mildew Resistance in Transgenic Vitis vinifera L.

et al. 2016

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Pathogenesis-related proteins (PRs) can lead to increased resistance of the whole plant to pathogen attack. Here, we isolate and characterize a PR-4 protein (VpPR4-1) from a wild Chinese grape Vitis pseudoreticulata which shows greatly elevated transcription following powdery mildew infection. Its expression profiles under a number of abiotic stresses were also investigated. Powdery mildew, salicylic acid, and jasmonic acid methyl ester significantly increased the VpPR4-1 induction while NaCl and heat treatments just slightly induced VpPR4-1 expression. Abscisic acid and cold treatment slightly affected the expression level of VpPR4-1. The VpPR4-1 gene was overexpressed in 30 regenerated V. vinifera cv. Red Globe via Agrobacterium tumefaciens-mediated transformation and verified by the Western blot. The 26 transgenic grapevines exhibited higher expression levels of PR-4 protein content than wild-type vines and six of them were inoculated with powdery mildew which showed that the growth of powdery mildew was repressed. The powdery mildew-resistance of Red Globe transformed with VpPR4-1 was enhanced inoculated with powdery mildew. Moreover, other powdery mildew resistant genes were associated with feedback regulation since VpPR4-1 is in abundance. This study demonstrates that PR-4 protein in grapes plays a vital role in defense against powdery mildew invasion.

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“…Since 1999, in different genetic backgrounds several genes that may contribute to PM resistance have been identified, such as VpGLOX (Guan et al, 2011); VpPR-10.1 (Xu et al, 2010b; Xu et al, 2014); VpRFP1 (Yu et al, 2011); VpALDH2B4 (Wen et al, 2012); VpNAC1 (Zhu et al, 2012b); VpPR4-1 (Dai et al, 2016) ( Tables 2A and S2A). Many of them are transcription factors (Zhu et al, 2012a; Zhu et al, 2012b), as expected since they are master regulators in controlling plant response to biotic stress (Amorim et al, 2017; Ng et al, 2018).…”

Section: Grapevine-ascomycete Interactionmentioning

confidence: 99%

Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

et al. 2019

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The reduction of pesticide usage is a current imperative and the implementation of sustainable viticulture is an urgent necessity. A potential solution, which is being increasingly adopted, is offered by the use of grapevine cultivars resistant to its main pathogenic threats. This, however, has contributed to changes in defense strategies resulting in the occurrence of secondary diseases, which were previously controlled. Concomitantly, the ongoing climate crisis is contributing to destabilizing the increasingly dynamic viticultural context. In this review, we explore the available knowledge on three Ascomycetes which are considered emergent and causal agents of powdery mildew, black rot and anthracnose. We also aim to provide a survey on methods for phenotyping disease symptoms in fields, greenhouse and lab conditions, and for disease control underlying the insurgence of pathogen resistance to fungicide. Thus, we discuss fungal genetic variability, highlighting the usage and development of molecular markers and barcoding, coupled with genome sequencing. Moreover, we extensively report on the current knowledge available on grapevine-ascomycete interactions, as well as the mechanisms developed by the host to counteract the attack. Indeed, to better understand these resistance mechanisms, it is relevant to identify pathogen effectors which are involved in the infection process and how grapevine resistance genes function and impact the downstream cascade. Dealing with such a wealth of information on both pathogens and the host, the horizon is now represented by multidisciplinary approaches, combining traditional and innovative methods of cultivation. This will support the translation from theory to practice, in an attempt to understand biology very deeply and manage the spread of these Ascomycetes.

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Isolation and expression analysis of a novel pathogenesis-related protein 10 gene from Chinese wild Vitis pseudoreticulata induced by Uncinula necator

Cited by 13 publications

References 23 publications

A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

A Pathogenesis-Related Protein-Like Gene Is Involved in the Panax notoginseng Defense Response to the Root Rot Pathogen

The Novel Gene VpPR4-1 from Vitis pseudoreticulata Increases Powdery Mildew Resistance in Transgenic Vitis vinifera L.

Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

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