Isolation and expression of the gene encoding mitochondrial ADP/ATP carrier (AAC) from the pathogenic yeastCandida parapsilosis

Neboháčová, Martina; Mentel, Marek; Nosek, Jozef; Kolarov, Jordan

doi:10.1002/(sici)1097-0061(19990915)15:12<1237::aid-yea446>3.0.co;2-3

Cited by 10 publications

(7 citation statements)

References 12 publications

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“…This observation clearly distinguishes the transcription pattern of this second YlAAC isogene from the expression profile of the AAC genes in the other obligate aerobic yeast species. In K. lactis, C. parapsilosis, and S. pombe, transcription of the single AAC gene is down regulated under anaerobic conditions (Nebohacova et al 1999;Trezeguet et al 1999), as in the case of YlAAC1.…”

Section: Ylaac1 Is Down-regulated Under Hypoxic Conditionsmentioning

confidence: 99%

“…In the latter case, cells were harvested after 16 h of aerobic growth in the YPD medium, resuspended in fresh YPD medium and cultured semi anaerobically as described by Trezeguet et al (1999) and Nebohacova et al (1999).…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

“…In S. cerevisiae, both ScAAC1 and ScAAC2 are expressed preferentially in the presence of oxygen, while the ScAAC3 gene is expressed exclusively under hypoxic conditions (Kolarov et al 1990;Sabova et al 1993). In contrast to baker's yeast, other yeast species, such as Kluyveromyces lactis, Schizosaccharomyces pombe and Candida parapsilosis, possess only one AAC gene, which codes for a single enzyme form (Viola et al 1995;Couzin et al 1996;Nebohacova et al 1999). The latter three species are aerobic, petite Ànegative yeasts, and it has been suggested that their inability to grow under anaerobic conditions could be related to the absence of a hypoxic carrier isoform, similar to the ScAac3 in S. cerevisiae (Nebohacova et al 1999;Trezeguet et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to baker's yeast, other yeast species, such as Kluyveromyces lactis, Schizosaccharomyces pombe and Candida parapsilosis, possess only one AAC gene, which codes for a single enzyme form (Viola et al 1995;Couzin et al 1996;Nebohacova et al 1999). The latter three species are aerobic, petite Ànegative yeasts, and it has been suggested that their inability to grow under anaerobic conditions could be related to the absence of a hypoxic carrier isoform, similar to the ScAac3 in S. cerevisiae (Nebohacova et al 1999;Trezeguet et al 1999). To explore the rationale for the existence of single/multiple AAC genes in yeasts, we investigated the number and expression of ADP/ATP carrier-encoding gene(s) in the non-conventional yeast species Yarrowia lipolytica.…”

Section: Introductionmentioning

confidence: 99%

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Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygen

et al. 2005

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Yarrowia lipolytica is a strictly aerobic fungus, which differs from the extensively studied model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe with respect to its physiology, genetics and dimorphic growth habit. We isolated and sequenced cDNA and genomic clones (YlAAC1) from Y. lipolytica that encode a mitochondrial ADP/ATP carrier. The YlAAC1 gene can complement the S. cerevisiae Deltaaac2 deletion mutant. Southern hybridization, analysis of Yarrowia clones obtained in the course of the Genolevures project, and further sequencing revealed the existence of two paralogs of the YlAAC1 gene, which were named YlAAC2 and YlAAC3, respectively. Phylogenetic analysis showed that YlAAC1 and YlAAC2 were more closely related to each other than to YlAAC3, and are likely to represent the products of a recent gene duplication. All three Y. lipolytica YlAAC genes group together on the phylogenetic tree, suggesting that YlAAC3 is derived from a more ancient duplication within the Y. lipolytica lineage. A similar branching pattern for the three ScAAC paralogs in the facultative anaerobe S. cerevisiae demonstrates that two rounds of duplication of AAC genes occurred independently at least twice in the evolution of hemiascomycetous yeasts. Surprisingly, in both the aerobic Y. lipolytica and the facultative anaerobe S. cerevisiae, the three paralogs are differentially regulated in the absence of oxygen. Apparently, Y. lipolytica can sense hypoxia and down-regulate target genes in response.

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Section: Ylaac1 Is Down-regulated Under Hypoxic Conditionsmentioning

confidence: 99%

Section: Strains and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygen

et al. 2005

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“…The role of Aac1 is not completely clear; curiously, it has been implicated in the vacuolar accumulation of the red pigment formed by ade2 mutants [154]. Gene homologues to those encoding ATP translocators in S. cerevisiae have been cloned from K. lactis [155], Candida parasilopsis [156] and S. pombe . In this last yeast, the gene has been termed anc1 + [157].…”

Section: Oxidation Of Nadh and Energy Generationmentioning

confidence: 99%

Carbohydrate and energy-yielding metabolism in non-conventional yeasts: Figure 1

et al. 2000

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Sugars are excellent carbon sources for all yeasts. Since a vast amount of information is available on the components of the pathways of sugar utilization in Saccharomyces cerevisiae it has been tacitly assumed that other yeasts use sugars in the same way. However, although the pathways of sugar utilization follow the same theme in all yeasts, important biochemical and genetic variations on it exist. Basically, in most non-conventional yeasts, in contrast to S. cerevisiae, respiration in the presence of oxygen is prominent for the use of sugars. This review provides comparative information on the different steps of the fundamental pathways of sugar utilization in non-conventional yeasts: glycolysis, fermentation, tricarboxylic acid cycle, pentose phosphate pathway and respiration. We consider also gluconeogenesis and, briefly, catabolite repression. We have centered our attention in the genera Kluyveromyces, Candida, Pichia, Yarrowia and Schizosaccharomyces, although occasional reference to other genera is made. The review shows that basic knowledge is missing on many components of these pathways and also that studies on regulation of critical steps are scarce. Information on these points would be important to generate genetically engineered yeast strains for certain industrial uses.

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Isolation and expression of the gene encoding mitochondrial ADP/ATP carrier (AAC) from the pathogenic yeastCandida parapsilosis

Cited by 10 publications

References 12 publications

Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygen

Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygen

Carbohydrate and energy-yielding metabolism in non-conventional yeasts: Figure 1

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