Isolation and functional analysis of human HMBOX1, a homeobox containing protein with transcriptional repressor activity

Chen, S.; Saiyin, Hexige; Zeng, Xiaoqun; Jiang, Xi; Liu, X.; Li, Xiaofei; Yu, Lijuan

doi:10.1159/000093328

Cited by 44 publications

(50 citation statements)

References 50 publications

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“…Additionally, there was no significant change in hmBOx1 expression in the other cancer tissues tested. Similar to the results reported by chen et al (2), high levels of 3) were pulled down by mab 2a5F4, mab 4a4F2 or the ig isotype antibody, respectively. Complexes and input (non-immunoprecipitated whole cell extract) were detected by Western blot analysis using mAb 4A4F2 or mab 2a5F4 as described in materials and methods.…”

Section: Abnormal Expression Of Hmbox1 In Carcinoma Tissuessupporting

confidence: 88%

“…it is a member of the homeobox family of genes, which is characterized by the presence of a dna sequence, the homeobox (1). phylogenetic analyses have confirmed that HMBOX1 is more distantly related to hnF1a and hnF1B and has an atypical homeodomain possessing a 21-amino acid insertion between the second and third helix (2,3). Zhang et al (3) previously cloned a splicing variant of hmBOx1, designated hmBOx1b, from a human pancreatic cdna library.…”

Section: Introductionmentioning

confidence: 90%

“…hmBOx1 is highly conserved in humans, mice, rats, chickens and xenopus (2), and is detected in both the cytoplasm and nucleus of 10 normal human tissues, including the cerebrum, pancreas, kidney and liver (4). co-transfection of hEK-293t cells with the pm-hmBOx1 plasmid and reporter plasmid pGal4 5 tkluc indicates that the hmBOx1 protein may be a transcription repressor, while hmBOx1b only retains faint transcriptional repressive activity (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…co-transfection of hEK-293t cells with the pm-hmBOx1 plasmid and reporter plasmid pGal4 5 tkluc indicates that the hmBOx1 protein may be a transcription repressor, while hmBOx1b only retains faint transcriptional repressive activity (2,3). We also found that hmBOx1 exhibits negative regulatory effects on natural killer cell activation (unpublished data).…”

Section: Introductionmentioning

confidence: 99%

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Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies

Dai

Zhang

Tian

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. homeobox containing 1 (hmBOx1) is a novel transcription factor. however, the expression of hmBOx1 and its functions in human cancer tissues and cell lines have not been fully defined. We generated two specific monoclonal antibodies, 2a5F4 and 4a4F2, against human hmBOx1. in the present study, these two anti-hmBOx1 antibodies were used to investigate the protein expression profile of HMBOX1 in various human cancer tissues and cell lines. the results showed that hmBOx1 in kidney tissue was mainly expressed in the renal tubule; the expression level of hmBOx1 was much higher in clear-cell carcinoma of the kidney originating from the renal tubule. additionally, high levels of hmBOx1 protein were detected not only in pancreatic cancer tissue but also in the adjacent normal tissue. notably, the expression level of hmBOx1 in liver cancer was dramatically decreased compared with that in the adjacent normal tissue. abnormal expression of hmBOx1 in different types of carcinoma tissues suggests that hmBOx1 may be involved in the pathobiology of tumors.

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Section: Abnormal Expression Of Hmbox1 In Carcinoma Tissuessupporting

confidence: 88%

Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies

Dai

Zhang

Tian

et al. 2011

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…Human HMBOX1 is widely expressed in at least 18 tissues, and highly in pancreas, brain, placenta, prostate, thymus and testis. Co-transfection of HEK-293T cells with pM-HMBOX1 plasmid and reporter plasmid pGAL4 5 tkLUC indicates that HMBOX1 protein may be one transcription repressor, but how it works is unknown (1).…”

Section: Introductionmentioning

confidence: 99%

Recombinant Expression of a Novel Human Transcriptional Repressor HMBOX1 and Preparation of Anti-HMBOX1 Monoclonal Antibody

Dai

Zhang

et al. 2009

Cell Mol Immunol

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HMBOX1 was a novel transcription factor possibly involving in function of pancreas and cytotoxicity of NK cells. For function determination, recombinant human HMBOX1 protein was obtained and purified, and the monoclonal antibodies against HMBOX1 were prepared. The full-length cDNA fragment encoding HMBOX1 was amplified from NK-92 cells and inserted into prokaryotic expression vector pET22b. The pET22b-HMBOX1-6his vector was then transformed into E. coli Rosetta (DE3) and induced by 1 mM IPTG for 4 h at 37 o C. The fusion HMBOX1 protein was mainly expressed in inclusion bodies, which was purified and refolded using Ni 2+ -affinity chromatography. With the purified fusion HMBOX1 protein as antigen, monoclonal antibodies against HMBOX1 were generated, providing a potentially useful tool for further study in HMBOX1 functions. Using these anti-HMBOX1 mAbs, we identified that HMBOX1 is located in both cytoplasm and nucleus and could be detected in 10 human normal tissues, including cerebrum, pancreas, kidney and liver tissues. Moreover, the expression in hepatic carcinoma was significantly lower than that in adjacent tissues. Cellular & Molecular Immunology. 2009; 6(4):261-268.

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Primary NTRK‐rearranged spindle cell neoplasm of bone harboring an HMBOX1::NTRK3 gene fusion

Wang

2023

Genes Chromosomes & Cancer

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The majority of neurotrophic tyrosine receptor kinase (NTRK) rearranged neoplasms occur either in the superficial or deep soft tissues of extremities or trunk. Occasionally, it arises in visceral organs. However, its occurrence as a primary osseous tumor has not been documented thus far. Herein, we describe a unique case of an NTRK rearranged neoplasm that presented as a primary bony lesion. The tumor occurred in a 21-year-old woman who presented with an increasing pain in the right lower extremity. Radiologic examinations revealed a destructive lytic lesion located in the lower portion of the right femur. Histologically, the tumor was composed of haphazard fascicles of monomorphic spindle cells displaying mild nuclear atypia and rare mitotic activity. Immunohistochemically, the tumor cells showed focal staining of pan-TRK and S100 protein. Fluorescence in situ hybridization analysis was performed with the utilization of break-apart probes for NTRK1/NTRK2/NTRK3 genes. An NTRK3 rearrangement was identified. Subsequent next-generation sequencing (RNA-seq) revealed HMBOX1exon6::NTRK3exon 14 fusion.Our study illustrates, albeit extremely rare, that NTRK-rearranged neoplasms can arise as a primary bone lesion. In addition, we describe a novel HMBOX1::NTRK3 fusion that has not been documented before.

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Isolation and functional analysis of human HMBOX1, a homeobox containing protein with transcriptional repressor activity

Cited by 44 publications

References 50 publications

Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies

Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies

Recombinant Expression of a Novel Human Transcriptional Repressor HMBOX1 and Preparation of Anti-HMBOX1 Monoclonal Antibody

Primary NTRK‐rearranged spindle cell neoplasm of bone harboring an HMBOX1::NTRK3 gene fusion

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