Isolation and functional characterization of the FLOWERING LOCUS T homolog, the LsFT gene, in lettuce

Fukuda, Machiko; Matsuo, Satoshi; Kikuchi, Kaori; Kawazu, Yoichi; Fujiyama, Ryoi; Honda, Ichiro

doi:10.1016/j.jplph.2011.02.004

Cited by 46 publications

(48 citation statements)

References 27 publications

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“…Compared with the Arabidopsis ft-1 mutants, the early-flowering phenotype and production of less rosette leaves were observed in ft-1 35S:PhFT transgenic plants ( Figure 6A and B). This was similar to Arabidopsis and other plant species that ectopically express FT orthologs (Kardailsky et al, 1999;Kobayashi et al, 1999;Hou and Yang, 2009;Fukuda et al, 2011;Hecht et al, 2011;Laurie et al, 2011;Sun et al, 2011;Huang et al, 2012;Xiang et al, 2012). In addition, the ft-1 35S:PhFT transgenic plants lost siliques after flowering ( Figure 6C).…”

Section: Discussionsupporting

confidence: 80%

“…This result was different from that obtained in lettuce. In lettuce, the transcripts of FT were the most abundant in the largest leaves (Fukuda et al, 2011). More interestingly, the transcripts of PhFT in the roots under vegetative phase were the highest than in the leaves and flowers tested.…”

Section: Discussionmentioning

confidence: 89%

“…Similar to the FT genes from other plant species, the PhFT contained 3 introns and 4 exons (Figure 2). Although the sizes of FT introns vary across different plant species, the lengths of the second (62 bp) and third (41 bp) exons seem to be conserved across different plant species (Fukuda et al, 2011;Harig et al, 2012). PhFT had a highly conserved amino acid sequence from the N-terminal to C-terminal, which was concomitant with the FT proteins of C. goeringii, Oncidium Gower Ramsey, G. hirsutum, P. pyrifolia, O. rufipogon, P. mume, and H. vulgare subsp vulgare (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

“…The FT-like proteins that promote flowering have been confirmed in a series of dicotyledonous and monocotyledonous plants, such as sugar beet (Pin et al, 2010), sunflower (Blackman et al, 2010), pea (Hecht et al, 2011), soybean (Sun et al, 2011), medicago , lettuce (Fukuda et al, 2011), chrysanthemum (Oda et al, 2012), rice (Kojima et al, 2002), corn (Meng et al, 2011), and orchids such as Oncidium Gower Ramsey (Hou and Yang, 2009) and Chinese Cymbidium (Huang et al, 2012;Xiang et al, 2012). More recently, an antiflorigenic effect of FT-like genes on flowering was identified in several plant species.…”

Section: Introductionmentioning

confidence: 95%

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Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

Dm¹,

Fb²,

Gf³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Warm day and cool night conditions significantly induce reproductive spike formation in Phalaenopsis plants; hence, determining the flowering mechanism regulating the reproductive transition is important. Flowering locus T (FT) plays important roles in flowering induction in several plants. To explore spike induction by warm days and cool nights in Phalaenopsis orchids, we isolated the FT (PhFT) from Phalaenopsis hybrid Fortune Saltzman. The cDNA of PhFT was 809-bp long and contained a 531-bp open reading frame encoding a putative protein of 176 amino acids, a 58-bp 5'-untranslated region (UTR), and a 220-bp 3'-UTR. The predicted molecular mass of PhFT was 19.80 kDa, with an isoelectric point of 8.68. The PhFT was predicted to possess the conserved functional regions of the phosphatidylethanolamine-binding protein superfamily. Nucleotide sequence data indicated that PhFT contained 3 introns and 4 exons. Sequence alignment and phylogenetic analyses of PhFT revealed high homology to the FT proteins of Cymbidium goeringii and Oncidium Gower Ramsey. Quantitative realtime polymerase chain reaction analysis indicated that PhFT mRNA Functional characterization of a PhFT gene was expressed in roots, apical leaves, mature leaves, and flowers. In flowers, PhFT was expressed more in developing floral buds than in mature flowers and was predominantly expressed in ovaries and petals. Ectopic expression of PhFT in Arabidopsis ft-1 mutants showed novel early-flowering phenotypes that lost their siliques. Our results indicated that the ectopic expression of PhFT could partially complement the late flowering defect in transgenic Arabidopsis ft-1 mutants. Our findings suggest that PhFT is a putative FT homolog in Phalaenopsis plants that regulates flowering transition.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

Dm¹,

Fb²,

Gf³

et al. 2014

Genet. Mol. Res.

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“…Reports on FT genes isolated from several plants have shown that they are involved in flowering (Hayama et al, 2007;Lifschitz et al, 2006;Oda et al, 2012;Vergara et al, 2016;Xia et al, 2012). In a previous study, we isolated and characterized the L. sativa FT homolog (LsFT) (Fukuda et al, 2011). As LsFT overexpression induced early flowering in transgenic A. thaliana, LsFT seems to have a similar function to FT in the floral induction pathway.…”

Section: Introductionmentioning

confidence: 91%

Isolation and Gene Expression Analysis of Flowering-related Genes in Lettuce (<i>Lactuca sativa</i> L.)

et al. 2017

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Lettuce (Lactuca sativa L.) bolting is often limiting in agricultural production as stem elongation followed by flower bud differentiation results in unmarketable plants. Thus, to solve this problem, it is important to elucidate the molecular mechanisms controlling flowering. To investigate the relationship between gene expression and flowering in lettuce, homologs of flowering-related genes were isolated and their expression over time was analyzed in three cultivars and in one breeding line by using quantitative real-time PCR. In plants grown in the field, the expression of the lettuce homologues APETALA 1 (LsAP1L) and LEAFY (LsLFYL) in the shoot apex correlated with flower bud formation, and FLOWERING LOCUS T (LsFT) expression increased during the transition from vegetative to reproductive growth. The transcription of FLOWERING LOCUS D (LsFLDL), FVE (LsFVEL), and LUMINIDEPENDENS (LsLDL) also increased with flowering. Our previous study showed that LsFT was upregulated during lettuce flowering induced under controlled high temperature conditions. Results from both studies suggest that LsFT is involved in lettuce flowering, both in natural and controlled conditions. This is also the first report on the expression of other flowering-related genes along with flowering of lettuce grown in the field.

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Genome-wide association mapping reveals loci for shelf life and developmental rate of lettuce

et al. 2020

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Isolation and functional characterization of the FLOWERING LOCUS T homolog, the LsFT gene, in lettuce

Cited by 46 publications

References 27 publications

Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

Isolation and Gene Expression Analysis of Flowering-related Genes in Lettuce (<i>Lactuca sativa</i> L.)

Genome-wide association mapping reveals loci for shelf life and developmental rate of lettuce

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