Isolation and identification of a new antibacterial entity from the Antarctic cyanobacterium Nostoc CCC 537

Abstract

The present study was aimed at the isolation, purification and structural elucidation of an antibacterial entity/lead molecule from the Antarctic cyanobacterium Nostoc CCC 537. A methanolic extract of the cyanobacterium was bioassayed with Enterobacter aerogenes as a target. The extract was purified by TLC, and the most active band was subjected to HPLC. The fraction (retention time 15.7 min) designated as the active principle was antibacterial towards Gram positive Mycobacterium tuberculosis H37Rv, Staphylococcus aureus ATCC 25923, Gram negative Salmonella typhi MTCC 3216, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25992, Enterobacter aerogenes MTCC 2822 and multi-drug resistant strains of Escherichia coli GS 2003/01, 02, 03.

Isolation of a broad spectrum antibiotic producer bacterium, Pseudoalteromonas piscicida PG-02, from the Persian Gulf

“…Solvent was removed at 37°C. Screening for antibacterial activity: Freshly grown colonies of tested bacterial pathogens were used to inoculate Muller Hinton Broth (MHB, Merck) medium at 37°C for 5-8 hours until turbidity of the suspension was adjusted to the Mc Farland 0.5 turbidity standard (10 8 cfu/mL) (Mulks et al, 1990;Asthana et al, 2009). Antibacterial activity was assessed two times by disc diffusion method.…”

Identification, Isolation, and Characterization of Developmental Toxins from the Cyanobacterium Fischerella 52-1 Using the Zebrafish (Danio rerio) Embryo Model

“…One of the embryos was examined and removed from the chorion (Figure 18). The embryo exhibited a pericardial edema and curved body similar to previous observations of region 3. …”

Untitled

“…They are well known for antibacterial activity 36 . Asthana et al, identified naphthalene compound from Nostoc CCC 537 which has antibacterial property 37 .…”