Isolation and Identification of Antimicrobial Compounds against Helicobacter pylori from Rosemary (Rosmarinus officinalis L.) Extracts

Abstract

Rosmarinus officinalis extracts had a significant antimicrobial activity against Helicobacter pyori. Total phenolic contents and inhibition zone of rosemary extracts were estimated to be 25.7 mg/g and 14 mm at 200 µg/mL of phenolic contents, respectively. The Sephadex LH-20 and MCI-gel CHP-20 column chromatographic separations for the phenolic extracts from R. officinalis leaves led to isolation of five acids, whose structures were determined as protocatechuic acid (A), coumaric acid (B), caffeic acid (C), chlorogenic acid (D), and rosmarinic acid (E), from interpretation of spectroscopic data including nagative fast atom bombardment (FAB)-mass, 1 H-NMR, 13 C-NMR, and IR. All isolated compounds were tested for antimicrobial activity against H. pyori. The purified single compound showed less antimicrobial activity against H. pylori than the mixed purified compounds, which generate A+B, A+E, C+D, C+E (each 200 µg/disc) excellent as large clear zone by synergy effect. These results indicate rosemary extracts are preventive agents against H. pyori.

Inhibitory Activity against Helicobacter pylori of Isolated Compounds from Pinus koraiensis Siebold et Zucc Leaves

“…Therefore, as antimicrobial activities with extracts are more efficient than treatment with are fined single compound, extracted compounds are more suitable for industrial applications. These results are in line with previous studies demonstrating a synergistic effect of mixing several compounds of rosemary (Yoon et al, 2011), Morus alba L. (Yun et al, 2010), and Rhododendron mucronulatum (Ju and to increase the antimicrobial activity against H. pylori.…”

“…As shown in C-NMR spectrum. Compound B was identified as p-coumaric acid, which corroborates the analysis of Yoon et al (2011) (Fig. 2B).…”

“…Helicobacter pylori is the principal cause of chronic gastritis, leading to peptic and duodenal ulcers, as well as gastric cancer (Suerbaum and Michetri, 2002;Park et al, 2003;Kang and Lee, 2005). Since the World Health Organization designated H. pylori as the first carcinogenic factor of gastric cancer, research on H. pylori has increased and much progress has been made in understanding its effects (Kim, 2006;Park et al, 2010;Yun et al, 2010;Kim andCho, 2011a, 2011b;Yoon et al, 2011;Park et al, 2012). The inflammation rate caused by H. pylori is high in normal adults, at 60−75% (Kang and Lee, 2005).…”