Isolation and Identification of Naturally Occurring Larvicidal Compound Isolated From Zingiber Zerumbet (L).J.E. Smith.

Abstract

Objective: A naturally occurring larvicidal compound is an alternative to eradication of Aedes aegypti larvae. This compound is toxic to larvae but relatively safe for human. Rhizome of Lempuyang gajah (Zingiber zerumbet (L.) J. E. Smith) has been used traditionally to prevent mosquito bites. An initial study indicated that petroleum ether (PE) soluble fraction of the methanol (MeOH) extract of Z. zerumbet was toxic against larvae lethal concentration (LC 50 ), 67.01±2.35 versus 153.57±4.01 ppm (MeOH ext.). Therefore, this study aimed to isolate and identify the compounds with larvicidal activity from Z. zerumbet rhizome. Methods:The PE soluble (PE-soluble) fraction was subjected to a bioassay-guided fractionation and isolation method to obtain four (4) fractions (I-IV). Two compounds (1 and 2) were isolated from Fraction I that was the most active compared to other fractions (II-IV). Compound 1 turned out to be more active than compound 2; therefore, compound 1 was then identified by means of spectroscopic data. Results Conclusion:Zerumbone was the main active compound; in the future, this compound can be formulated as a standardized preparation based on the content.

Bioassay Guided Fractionation of Ciplukan (Physalis angulata L.) Monitored by Glucose Consumption Assay and Thin Layer Chromatography on Myoblast Cells

“…The bioassay-guided fractionation method has been used previously to test the anticancer activity of compounds isolated from N. indicum leaves (Wahyuningsih et al, 2017), detecting the presence of the active fraction of T. diversifolia (Hemsley) A Gray which has antiplasmodial potential (Syarif et al, 2018). This method is widely used in the early stages of isolated compounds from natural materials, such as tagitinin C from T. diversifolia (Wahyuningsih et al, 2015) and Zerumbone from Zingiber zerumbet (Murini et al, 2018).…”

The ability of active fraction from Kappaphycus alvarezii (Doty) Doty Ex.P.C.Silva to Decrease Glucose Levels inhibit advanced Glycation End-Products (AGEs) Formation and RAGE (AGER) Gene Expression in Diabetic Rats

“…This bioassayguided fractionation method had been applied to detect the presence of active fraction from T. diversifolia (Hemsley) A Gray which has antiplasmodial potential [13]. İt has been widely used for the initial stages in the isolation of compounds from natural materials, such as the compound tagitinin C from T. diversifolia [14] and Zerumbone from Zingiber zerumbet [15].…”

The effect of Kappaphycus alvarezii fraction on plasma glucose, Advanced Glycation End-products formation, and renal RAGE gene expression

“…Gray, which have antiplasmodial potential ( Syarif et al., 2018 ). Moreover, it has been widely used in the initial isolation stages of compounds from natural materials, such as the tagitinin C compound from T. diversifolia ( Wahyuningsih et al., 2015 ) and zerumbone from Zingiber zerumbet ( Murini et al., 2018 ).…”