Isolation and Identification of Pathogenic Vibrio Species in Black Rockfish Sebastes schlegeli

Liu, Xiaoling; You, Cuirong; Zeng, Yong

doi:10.3390/fishes8050235

Cited by 2 publications

(1 citation statement)

References 51 publications

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“…Analysis of 16S rRNA sequences from Vibrio species isolated from aquaculture waters showed that V. harveyi (25%), V. anguillarum (12.5%), and V. scophthalmi (12.5%) had higher rates compared to other Vibrio species. These species are already frequently isolated during vibriosis outbreaks [49,50]. Vibrio harveyi is frequently found in shrimp cultures, whereas Vibrio anguillarum is commonly associated with disease in sea bass and trout [51][52][53].…”

Section: Discussionmentioning

confidence: 99%

Partial Characterization of Three Bacteriophages Isolated from Aquaculture Hatchery Water and Their Potential in the Biocontrol of Vibrio spp.

Yaşa,

Evran,

Eren Eroğlu

et al. 2024

Microorganisms

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Bacteriophages are currently considered one of the most promising alternatives to antibiotics under the ‘One Health’ approach due to their ability to effectively combat bacterial infections. This study aimed to characterize Vibrio species in hatchery water samples collected from an aquaculture farm and investigate the biocontrol potential of their bacteriophages. Vibrio spp. (n = 32) isolates confirmed by LNA probe-based qPCR were used as hosts. Three Vibrio phages were isolated. IKEM_vK exhibited a broad host range, infecting V. harveyi (n = 8), V. alginolyticus (n = 2), V. azureus (n = 1), and V. ordalii (n = 1). IKEM_v5 showed lytic activity against V. anguillarum (n = 4) and V. ordalii (n = 1), while IKEM_v14 was specific to V. scophtalmi (n = 4). The morphological appearance of phages and their lytic effects on the host were visualized using scanning electron microscopy (SEM). All three phages remained relatively stable within the pH range of 6–11 and up to 60 °C. The lytic activities and biofilm inhibition capabilities of these phages against planktonic Vibrio cells support their potential applications in controlling vibriosis in aquaculture systems.

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Section: Discussionmentioning

confidence: 99%

Partial Characterization of Three Bacteriophages Isolated from Aquaculture Hatchery Water and Their Potential in the Biocontrol of Vibrio spp.

Yaşa,

Evran,

Eren Eroğlu

et al. 2024

Microorganisms

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Heat stress impacts the gut microbiome of Atlantic salmon by promoting growth of Vibrionaceae and is associated with extensive cast production

Bowman

2024

Preprint

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Atlantic salmon (Salmo salar) when exposed to heat stress have reduced voluntary feeding and exhibit changes in digesta consistency. This study was performed to determine what bacterial species occur and the overall bacterial abundance in the gut microbiome in heat stressed Atlantic salmon. For this Atlantic salmon in seawater tanks at 15°C were fed for 2 to 4 weeks. Tank temperatures were increased to 19°C until voluntary feeding abated at which point tank temperatures were cooled to 15°C for 4 weeks. At the end of each temperature phase the fish were stripped of feces and microbiome profiles were determined using 16S rRNA V1-V3 metabarcoding. The tank experiment was repeated three times in successive years. Vibrionaceae comprised most reads after the warm phase completed. The prominent levels of Vibrionaceae were accompanied by a large predominance of cast (sloughed intestinal mucosa) containing fecal samples. qPCR estimated Vibrionaceae cell populations increased 1.9-3.4 log units/g after the warm phase but and slightly decreased by 0.3-1.1 log units/g after the 15°C recovery phase. The results indicated heat stress induced inappetence corresponds to increased cast production accompanied by predominance of Vibrionaceae. Vibrionaceae colonizing the Atlantic salmon gut should be the focus of studies on the microbiology of thermally induced inappetence and dysbiosis in Atlantic salmon.

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Isolation and Identification of Pathogenic Vibrio Species in Black Rockfish Sebastes schlegeli

Cited by 2 publications

References 51 publications

Partial Characterization of Three Bacteriophages Isolated from Aquaculture Hatchery Water and Their Potential in the Biocontrol of Vibrio spp.

Partial Characterization of Three Bacteriophages Isolated from Aquaculture Hatchery Water and Their Potential in the Biocontrol of Vibrio spp.

Heat stress impacts the gut microbiome of Atlantic salmon by promoting growth of Vibrionaceae and is associated with extensive cast production

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