Isolation and identification of quorum sensing antagonist from Cinnamomum verum leaves against Pseudomonas aeruginosa

Alva, Prathiksha Prabhakara; Suresh, Sarika; Nanjappa, Dechamma Pandyanda; James, Jainey Puthenveetil; Kaverikana, Rajesh; Chakraborty, Anirban; Sarojini, B. K.; Premanath, Ramya

doi:10.1016/j.lfs.2020.118878

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…In this regard, the authors detected reduced P. aeruginosa ability in producing pyocyanin, elastase, swarming motility, and biofilm formation. Lower concentrations (below 100 mg/L) of C. verum compound did not show any toxicity on zebrafish embryos ( Alva et al., 2021 ).…”

Section: Inhibitory Effects Of Cinnamomum On Micro...mentioning

confidence: 99%

Cinnamomum: The New Therapeutic Agents for Inhibition of Bacterial and Fungal Biofilm-Associated Infection

Didehdar

Chegini

Tabaeian

et al. 2022

Front. Cell. Infect. Microbiol.

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Due to the potent antibacterial properties of Cinnamomum and its derivatives, particularly cinnamaldehyde, recent studies have used these compounds to inhibit the growth of the most prevalent bacterial and fungal biofilms. By inhibiting flagella protein synthesis and swarming motility, Cinnamomum could suppress bacterial attachment, colonization, and biofilm formation in an early stage. Furthermore, by downregulation of Cyclic di‐guanosine monophosphate (c‐di‐GMP), biofilm-related genes, and quorum sensing, this compound suppresses intercellular adherence and accumulation of bacterial cells in biofilm and inhibits important bacterial virulence factors. In addition, Cinnamomum could lead to preformed biofilm elimination by enhancing membrane permeability and the disruption of membrane integrity. Moreover, this substance suppresses the Candida species adherence to the oral epithelial cells, leading to the cell wall deformities, damage, and leakages of intracellular material that may contribute to the established Candida’s biofilm elimination. Therefore, by inhibiting biofilm maturation and destroying the external structure of biofilm, Cinnamomum could boost antibiotic treatment success in combination therapy. However, Cinnamomum has several disadvantages, such as poor solubility in aqueous solution, instability, and volatility; thus, the use of different drug-delivery systems may resolve these limitations and should be further considered in future investigations. Overall, Cinnamomum could be a promising agent for inhibiting microbial biofilm-associated infection and could be used as a catheter and other medical materials surface coatings to suppress biofilm formation. Nonetheless, further in vitro toxicology analysis and animal experiments are required to confirm the reported molecular antibiofilm effect of Cinnamomum and its derivative components against microbial biofilm.

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Section: Inhibitory Effects Of Cinnamomum On Micro...mentioning

confidence: 99%

Cinnamomum: The New Therapeutic Agents for Inhibition of Bacterial and Fungal Biofilm-Associated Infection

Didehdar

Chegini

Tabaeian

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Tarçın yağının P. aeruginosa'da QS kontrollü virülans faktörleri ve biyofilm oluşumu üzerindeki etkisinin araştırıldığı çalışmada tarçın yağının biyofilm oluşumunu engellediği ve çok düşük konsantrasyonlarda bile (0.1-0.2 µl/ml) QS'i inhibe ettiği gösterilmiştir (Kalia, 2015). Yakın zamanda yapılan yine benzer bir çalışmada da C. verum'dan elde edilen saflaştırılmış bileşikler, 0.1 mg/ml'lik bir konsantrasyonda P. aeruginosa üzerinde hem antibiyofilm hem de anti-QS etkiye sahip olduğu görülmüştür (Alva, 2021). Bu alanda yapılan çalışmalar sınırlı olup C. verum yaprağı yağının antibiyofilm ve anti-QS etkisi ile ilgili başka bir çalışmaya rastlanılmamıştır.…”

Section: Kırayunclassified

“…P. aeruginosa'nın şiddeti, biyofilm oluşturma yeteneğiyle birlikte elastaz, piyosiyanin, eksotoksinler, alkalin proteaz, ramnolipidler ve sideroforlar gibi çeşitli virülans faktörlerinin üretimi ve salgılanmasından kaynaklanmaktadır. P. aeruginosa'da bu virülans faktörlerinin üretimi, QS sisteminin kontrolü altında bulunmaktadır (Alva, 2021). Yapılan çalışmalarda çoklu ilaca dirençli Acinetobacter ve P. aeruginosa'ya karşı anti-QS bileşiklerinin izolasyonu ile ilgili raporlar sınırlıdır.…”

Section: Introductionunclassified

Anti-quarum Sensing Activity of Cinnamomum verum Essential Oil with Antibacterial and Antibiofilm Effect on Pseudomonas aeruginosa and Acinetobacter baumannii

Kiray

2022

BAUN Sağ Bil Derg

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Objective: The emergence of multi-drug-resistant pathogens on antimicrobials in current use has made it necessary to search for alternative treatment strategies. Plant essential oils are considered as alternative antimicrobials in the treatment of diseases because they are non-toxic, cost-effective and accessible. This study aimed to evaluate the antibacterial and antibiofilm effect and anti-quarum sensing (QS) properties of Cinnamomun verum leaf essential oil on Pseudomonas aeruginosa and Acinetobacter baumannii. Materials and Methods: In the study, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of EO on P. aeruginosa and A. baumannii were determined. The microdilution method was used to determine the minimum inhibitory concentrations (MICs). The chemical content of C. verum EO was determined using Gas Chromatography-Mass Spectrometry (GC-MS). Results: C. verum EO showed MIC and MBC at 0.5 and 1.0 mg/mL versus strains tested. It was determined that C. verum EO showed high anti-QS activity at a concentration of 2 mg/mL. According to GC-MS results, cinnamaldehyde was found to be the most abundant compound in C. verum EO. Conclusion: C. verum EO has potential to be used against infections caused by bacterial biofilms. Studies on the anti-QS activity of C. verum EOs are limited, and the anti-QS activity on pathogenic microorganisms should be investigated in this area.

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Phytochemistry and Pharmacological Studies of Indian Cinnamomum Schaeff

Surendran,

Ramasubbu

2023

Bioprospecting of Tropical Medicinal Plants

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Isolation and identification of quorum sensing antagonist from Cinnamomum verum leaves against Pseudomonas aeruginosa

Cited by 7 publications

References 34 publications

Cinnamomum: The New Therapeutic Agents for Inhibition of Bacterial and Fungal Biofilm-Associated Infection

Cinnamomum: The New Therapeutic Agents for Inhibition of Bacterial and Fungal Biofilm-Associated Infection

Anti-quarum Sensing Activity of Cinnamomum verum Essential Oil with Antibacterial and Antibiofilm Effect on Pseudomonas aeruginosa and Acinetobacter baumannii

Phytochemistry and Pharmacological Studies of Indian Cinnamomum Schaeff

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