Isolation and Identification of Staphylococcus aureus from Skin and Soft Tissue Infection in Sepsis Cases, Odisha

Mohanty, Anima; Mohapatra, KC; Pal, Bibhuti Bhusan

doi:10.22207/jpam.12.1.49

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…As per current study S. aureus infection rates 29.4% male verses 4.54% female, also MRSA male higher 11.36% than female 2.27%, some researchers reported male S. aureus infection rates higher than female [50]in India and UK male 57.5% female 42.2%, others find the contrary [51] in Asfahan find male infection ratio 43.8 % less than female 56.2%, [52] ,in Ethiopia found 33.2% in males less than females 68.8%.On the other hand [53]thoughts that chemicals and cosmetic material might play roles. Despite [39],reported MRSA rates were lower in males than females, on other hand [54], believed that estrogen had tough roll and influence upon skin thickness, prevent infections with S. aureus, role of sex hormones in modulation immune response and susceptibility for infection among males and females, steroid hormones have important character in skin physiology, immun-ity, skin architecture, thickness of dermis and epidermis layers [55,56] .…”

Section: F Contact With Petssupporting

confidence: 68%

Sociodemographic Risk Factors for Dermal Infections with Methi-cillin Sensitive and Methicillin Resistant Staphylococcus aureus among Sheep Breeders in Diyala Governorate , Iraq

Fajer¹,

Al-Ezzy²,

AL-Zuhairi³

2023

djm

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Background: Staphylococcus aureus is one of the dominant pathogen among skin infections in human and animals. Objective: To isolation and identification of S.aureus from sheep breeders by traditional method &Automated Vitek 2 system ,Detection of methicillin resistant gene (mecA) by conventional PCR. Evaluation of relationship between S.aureus in-fection and possible risk factors in human (age, sex, education level, economic statue, hospitalization in last four weeks contact with sheep skin lesions, and contact with pets). Patients and Methods: A total of 44 skin swaps collected from sheep breeders suffered from variety of infected skin lesions (wounds, abscesses, atopic dermatitis, acne, chronic skin diseases etc.) recording; sex, age, education level, economic statue, hospitaliza-tion in last four weeks, contact with sheep skin lesions, and contact with pets to detect S. aureus, MRSA and estimating the risk factors, by employing traditional laboratories methods in addition to confirmatory techniques by VETEK2 sys-tem and PCR, using specific primers (Staur 4, 6), MRSA gene primers (mecA). Results: Methicillin sensitive S. aureus was reported in 34.09% versus40% for MRSA among sheep breeders. Significant correlation reported between age groups and MRSA infections. Although, the rate of infection with methicillin sensitive S. aureus and MRSA was higher among males compared with females , no signifi-cant correlation reported between sex and infection with methicillin sensitive S. aureus and MRSA. Female breeders were at high risk for getting dermal MRSA infections compared with males. No significant correlation between the educa-tion level, economic status, contact with pets and methicillin sensitive S. aure-us , MRSA infections as Risk factors. Methicillin sensitive S. aureus infections among breeders significantly correlated with hospitalization in last four weeks .Significant correlation reported between contact with skin lesions of sheep and methicillin sensitive S. aureus , MRSA infections among breeders. Conclusion: Methicillin resistant trait was common among S.aureus isolated from sheep breeders .MRSA infections correlated with age. Female sheep breeders were at high risk for getting S.aureus and MRSA infections. Sex , education level, eco-nomic status, contact with pet play no role in infection with S.aureus. hospitali-zation in last four weeks, contact with skin lesions correlated significantly with S. aureus, MRSA infections among breeders.

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Section: F Contact With Petssupporting

confidence: 68%

Sociodemographic Risk Factors for Dermal Infections with Methi-cillin Sensitive and Methicillin Resistant Staphylococcus aureus among Sheep Breeders in Diyala Governorate , Iraq

Fajer¹,

Al-Ezzy²,

AL-Zuhairi³

2023

djm

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“…Staphylococcus aureus is an opportunist human pathogen responsible for numerous types of infections, from skin infections, such as abscesses or infected wounds, to life-threatening conditions, such as endocarditis, osteomyelitis, or septicemia [1]. Some S. aureus strains can be quite virulent due to the combined action of several virulence factors, the most important being Panton-Valentine leukocidin (PVL) and toxic shock syndrome toxin, associated with immune evasion, tissue adhesion, and host cell injury [2].…”

Section: Introductionmentioning

confidence: 99%

Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance

et al. 2020

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In this study we aimed to characterize antimicrobial resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolated from bloodstream infections as well as the associated genetic lineages of the isolates. Sixteen MRSA isolates were recovered from bacteremia samples from inpatients between 2016 and 2019. The antimicrobial susceptibility of these isolates was tested by the Kirby–Bauer disk diffusion method against 14 antimicrobial agents. To determine the macrolide–lincosamide–streptogramin B (MLSB) resistance phenotype of the isolates, erythromycin-resistant isolates were assessed by double-disk diffusion (D-test). The resistance and virulence genes were screened by polymerase chain reaction (PCR). All isolates were characterized by multilocus sequence typing (MLST), spa typing, staphylococcal chromosomal cassette mec (SCCmec) typing, and accessory gene regulator (agr) typing. Isolates showed resistance to cefoxitin, penicillin, ciprofloxacin, erythromycin, fusidic acid, clindamycin, and aminoglycosides, confirmed by the presence of the blaZ, ermA, ermC, mphC, msrA/B, aac(6’)-Ie-aph(2’’)-Ia, and ant(4’)-Ia genes. Three isolates were Panton–Valentine-leukocidin-positive. Most strains (n = 12) presented an inducible MLSB phenotype. The isolates were ascribed to eight spa-types (t747, t002, t020, t1084, t008, t10682, t18526, and t1370) and four MLSTs (ST22, ST5, ST105, and ST8). Overall, most (n = 12) MRSA isolates had a multidrug-resistance profile with inducible MLSB phenotypes and belonged to epidemic MRSA clones.

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“…21 Bacterial pathogens around the world are becoming increasingly resistant to antibiotics, rendering these drugs useless for treating infections in humans and animals. 22 The emergence of multidrug-resistant bacteria is being closely monitored. 23 The current study revealed that aloe-emodin had high antibacterial activity against human and cow pathogenic bacteria with high inhibition zone range increasing with increased aloe-emodin extract concentrations.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of aloe-emodin from Aloe-vera against Staphylococcus aureus

Ola Salam Znad,

Zainab Razzaq Zghair

2023

J Pak Med Assoc

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Objective: To evaluate the antibacterial activity of aloe-emodin isolated from aloe-vera against staphylococcus aureusfrom human patients and cows suffering from respiratory tract infection.Method: The experimental study was conducted at the A;-Kut Technical Institute, Iraq, from October 2021 to December2021, and comprised human nasal swabs collected from Al-Zahraa Teaching Hospital and Al-Karama Teaching Hospitalin Wassit, Iraq, in group A, cow nasal swabs collected from city centre, Al-Hay, Al-Bataar, Sheikh Saad, Al-Azizia and Al-Suwaira fields in group B. Aloe-vera was taken from the local market for which certification was obtained from theDirectorate of Seed Testing and Certification, Ministry of the Agriculture, Iraq. The swab specimens were transferredto the laboratory under standard conditions. The specimens were inoculated to mannitol salt agar and blood agarmedia and were then incubated for 24-48hrs at 37°C under aerobic conditions. All the primary screened isolates werethen subjected to various morphological and biochemical tests to ensure their identity. Data was analysed using thestatistical analysis system, 2018.Results: Of the 200 samples, 100(50%) were in group A and 100 100(50%) in group B. Aloe-emodin was the mosteffective antibiotic that inhibited human and cow pathogenic bacteria with high inhibition zone range at (1%) whichincreased with increasing aloe-emodin extract concentrations at (2% ,4%). Staphylococcus isolates revealed a differentresponse to the aloe-emodin antimicrobial activity.Conclusion: Aloe-emodin extract of alo-vera showed high antimicrobial activity against all human and cowpathogenic bacteria.Keywords: Cattle, Aloe, Emodin, Plant, Anti-bacterial, Anti-infective, Nose, Agriculture, Ethanol, Seeds.

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Isolation and Identification of Staphylococcus aureus from Skin and Soft Tissue Infection in Sepsis Cases, Odisha

Cited by 6 publications

References 16 publications

Sociodemographic Risk Factors for Dermal Infections with Methi-cillin Sensitive and Methicillin Resistant Staphylococcus aureus among Sheep Breeders in Diyala Governorate , Iraq

Sociodemographic Risk Factors for Dermal Infections with Methi-cillin Sensitive and Methicillin Resistant Staphylococcus aureus among Sheep Breeders in Diyala Governorate , Iraq

Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance

Antimicrobial activity of aloe-emodin from Aloe-vera against Staphylococcus aureus

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