ISOLATION AND IDENTIFICATION OF THE SUDANOPHILIC GRANULES OF
            <i>SPHAEROTILUS NATANS</i>

Rouf, M. A.; Stokes, J. L.

doi:10.1128/jb.83.2.343-347.1962

Cited by 41 publications

(15 citation statements)

References 12 publications

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“…On the basis of the above results, the refractile granules of strains B-106-4-4 and B-43-D-e were identified as PHB (15, 26,33).…”

Section: Resultsmentioning

confidence: 88%

“…Therefore, potassium-deficient and phosphate-rich media are preferable for PHB production. PHB production in bacterial cells has been reported for the following genera: Bacillus (1, 13-15, 32), Micrococcus (15, 29), Pseudomonas (4, 8,11,15), Azotobacter (8,15), Chromobacterium (8, 15), Spirillum (15), Rhodospirillum (4, 15; J. N. Baptist, U.S. patent 3,036,959, July 1962), Hydrogenomonas (28), Sphaerotilus (26), and Ferrobacillus (15). The physiological and biochemical roles of this bioplastic as a storage substance of carbon have been discussed by Schlegel and Gottschalk (27), Doudroff and Stanier (4), and Mulder et al (19), and the physical and chemical properties of the bioplastic have been summarized by Hester (10).…”

Section: Discussionmentioning

confidence: 99%

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Formation of Crystalline δ-Endotoxin or Poly-β-Hydroxybutyric Acid Granules by Asporogenous Mutants of Bacillus thuringiensis

Wakisaka

Masaki

Nishimoto

1982

Appl Environ Microbiol

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Parental strains and asporogenous mutants of Bacillus thuringiensis subspp. kurstaki and aizawai produced high yields of &-endotoxin on M medium, which contained 330 p.g of potassium per ml, but not on ST and ST-a media, each of which contained only 11 p.g of potassium per ml. On ST and ST-a media, refractile granules were formed instead. These granules had no insecticidal activity against silkworms and were isolated and identified as poly-p-hydroxybutyric acid. Supplementation of the potassium-deficient ST-a medium with 0.1% KH2PO4 (3.7 mM) led to the formation of crystalline 8-endotoxin. The replacement of KH2PO4 with equimolar amounts of KCl, KNO3, and potassium acetate or an equivalent amount of K2SO4 had a similar effect, whereas the addition of an equimolar amount of NaH2PO4 or NH4H2PO4 did not cause the endotoxin to form. An asporogenous mutant, B. thuringiensis subsp. kurstaki strain 290-1, produced 5endotoxin on ST-a medium supplemented with 3 mM or more potassium but formed only poly-3-hydroxybutyric acid granules on the media containing .1 mM potassium. These results clearly indicate that a certain concentration of potassium is essential for the fermentative production of 6-endotoxin by these isolates of B. thuringiensis. Manganese could not be substituted for potassium. Phosphate ions stimulated poly-,B-hydroxybutyric acid formation by strain 290-1. The sporulation of B. thuringiensis and several other Bacillus strains was suppressed on the potassium-deficient ST medium. This suggests that potassium plays an essential role not only in Bacillus cell growth and 5-endotoxin formation but also in sporulation.

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“…On the basis of the above results, the refractile granules of strains B-106-4-4 and B-43-D-e were identified as PHB (15, 26,33).…”

Section: Resultsmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Formation of Crystalline δ-Endotoxin or Poly-β-Hydroxybutyric Acid Granules by Asporogenous Mutants of Bacillus thuringiensis

Wakisaka

Masaki

Nishimoto

1982

Appl Environ Microbiol

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“…The scale for all figures is shown in section (j) and is equal to 1.0 IA. spectra of PHB reported for B. megaterium and 0.04) in the intrinsic viscosity of the various Sphaerotilus natans by Rouf and Stokes (1962). PHB samples (Table 2).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Poly-β-Hydroxybutyrate Extracted from Different Bacteria

et al. 1965

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different bacterial genera was studied with regard to its crystal structure, infrared absorption, intrinsic viscosity, and electron microscopy. All PHB samples precipitated from dilute chloroform solution gave identical X-ray diffractograms confirming uniformity of crystal structure, and uniformity of molecular structure, based on the similarity of the recorded infrared spectra, was also established. Crystal morphology was also similar, showing the reported "lath" shape structure for purified polymer from Bacillus cereus. Intrinsic viscosity ranged from 0.04 to 11.5 depending upon the polymer treatment; polymer molecular weights, based upon viscometry, could be estimated to range from 1,000 to 250,000. It is concluded that the same basic molecule is involved in all PHB present in the bacterial kindgom. ''no screen" X-ray film in a flat plate camera. Exposure times ranged from 1.5 to 15 hr, and film to-sample distance was 4 cm. For infrared-absorption analysis, enough poly-245

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“…(Busgen, 1894;Linde, 1913;Ellis, 1919;Stokes, 1954). Recently it has been shown that poly-P-hydroxybutyrate is the main constituent of these globules (Mulder & van Veen, 1962, 1963Mulder et al, 1962;Rouf & Stokes, 1962). When the nutrient medium contains H,S, sulphur globules may be formed within the cells of S. natans (Skerman, Dementjeva & Carey, 1957).…”

Section: The Reserve Materials Of Sheath Forming Bacteriamentioning

confidence: 99%

Iron Bacteria, particularly those of the Sphaerotilus‐Leptothrix Group, and Industrial Problems

Mulder¹

1964

Journal of Applied Bacteriology

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ISOLATION AND IDENTIFICATION OF THE SUDANOPHILIC GRANULES OF SPHAEROTILUS NATANS

Cited by 41 publications

References 12 publications

Formation of Crystalline δ-Endotoxin or Poly-β-Hydroxybutyric Acid Granules by Asporogenous Mutants of Bacillus thuringiensis

Formation of Crystalline δ-Endotoxin or Poly-β-Hydroxybutyric Acid Granules by Asporogenous Mutants of Bacillus thuringiensis

Characterization of Poly-β-Hydroxybutyrate Extracted from Different Bacteria

Iron Bacteria, particularly those of the Sphaerotilus‐Leptothrix Group, and Industrial Problems

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