Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Abstract

Objective: For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimens obtained during open or endoscopic surgery. Within the context of the present study our aim was to isolate cells from human urine by an alternative and safe technique rather than using the indicated method. Material and methods:After human urine samples had been collected from young and healthy donors via urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolation procedure, cells were characterized by immunocytochemical method using specific antibodies.Results: When these cells were characterized by immunocytochemical methods using specific antibodies some of them were positive for mesenchymal stem cell marker CD90 while the others were labelled with urothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells together with stem cells were observed in the urine-derived cell population. Conclusion:According to the results obtained from this study human urine may be preferred as an alternative stem cell and urothelial cell source in that this method is and easily reproducible non-invasive method.

Cells Involved in Urethral Tissue Engineering: Systematic Review

“…Those that were positive for CD90 were negative for CD45. This study demonstrated the presence of both cell types in fresh urine samples 14 .…”

Urine as a Main Effector in Urological Tissue Engineering—A Double-Edged Sword

“…It has been reported that by collecting fresh urine samples, the success rate of isolating and cultivating human urine-derived cells (HUDCs) can be improved. However, UDSC viability can be lost if the time of exposure is greater than 5 h, as pH will start to change and nutrients in urine will be lost [32].…”

“…In a study conducted by Tayhan SE et al, it has been shown that urine-derived cells (UDCs) contain both stem cells as well as urothelial cells. As a maker of urothelial cells, cytokeratin 7 was used, whereas a negative and positive marker for mesenchymal stem cells of human, CD90, and CD45, respectively, were used [32]. ADSCs and UDSCs have shown a similar positive display of approximately more than 92% for CD73, and CD44 while ADSCs showed higher expression for CD105 and CD90.…”

“…Dynamic conditions resulted in the formation of a multilayered mucosal structure similar to urothelial tissue. [40] On the Tayhan et al [32] studied both UDSCs and human urothelial cells that were isolated from fresh urine samples after been harvested from six healthy patients via catheterization. Using immunohistochemical assessment, UDSCs reached confluency within 12 days and were positive for cytokeratin 7.…”

Inducing differentiation of human urine‐derived stem cells into hepatocyte‐like cells by coculturing with human hepatocyte L02 cells

“…1 Studies showed that hUSCs had various biological characteristics of adult mesenchymal stem cells (MSCs) including clonogenicity, the expression of specific cell surface markers and multipotent differentiation ability. [1][2][3][4] HUSCs have been successfully isolated and continuous cultured in vitro in several laboratories. Because of a wide range of noninvasive sources and no ethical controversy, hUSCs have been induced to differentiate into various types of tissue cells such as osteoblasts, 5 chondrocytes, 6 adipocytes, 7 urothelial cells, 8 smooth muscle cells, 7 and nerve cells, 9 respectively.…”