Isolation and Molecular Characterization of Mycoplasma Isolates from Pneumonic Sheep and Goats in Andhra Pradesh

Isolation of Mycoplasma gallisepticum from the natural cases of chronic respiratory disease

“…All the positive growths were immediately sub cultured in PPLO agar plates after removal of contamination by filtering cultured broth medium with 0.22-micron syringe filter. Inoculated tubes with no turbidity and colour change were kept on maximum 21 days before discarding and considering them as negative (Whitford et al, 1994; Yadav et al, 2020) [5,6] . Positive growths from PPLO growth cultures were streaked into the PPLO agar base after filtration from syringe filter.…”

“…Plates were examined under microscope (40x and 100x) for the growth of characteristic colonies. Plates without visible growth of the organism were kept up to 21 days before discarding and considering them as negative (Yadav et al, 2020). Colonies suggestive of avian mycoplasmas (fried egg) subjected for confirmation by doing molecular diagnosis i.e., PCR.…”