Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the F17 fimbriae in bovine enterotoxigenic Escherichia coli

Lintermans, P.; Pohl, P.; Deboeck, Francine; Bertels, A.; Schlicker, Christine; Vandekerckhove, Joël; Damme, Jozef Van; Montagu, M. van; Greve, Henri De

doi:10.1128/iai.56.6.1475-1484.1988

Cited by 61 publications

(29 citation statements)

References 42 publications

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“…Prior to the challenge, the pathotypes of these two strains were checked by colony hybridisation with the appropriate gene probes (Mainil et al, 1997) and their serotypes by agglutination with specific antisera. Strain 25KH09 originally isolated from the faeces of a calf with diarrhoea (Pohl et al, 1982), is serotype O101:K þ :H À (Pohl et al, 1987), and is the reference strain for the F17a fimbriae (Lintermans et al, 1988a). This strain is considered non-pathogenic and was chosen as an adherent non-toxigenic control (Pohl et al, 1987;Pohl and Mainil, 1995).…”

Section: Methodsmentioning

confidence: 99%

Necrotoxigenic Escherichia coli type-2 invade and cause diarrhoea during experimental infection in colostrum-restricted newborn calves

Bost

Roels

Mainil

2001

Veterinary Microbiology

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There exists experimental evidence that necrotoxigenic Escherichia coli (NTEC) strains producing the cytotoxic necrotising factor 1 cause intestinal and extra-intestinal disease in piglets. On the other hand, no experimental model has been developed with NTEC strains producing the cytotoxic necrotising factor 2. In all, 14 colostrum-restricted calves were orally challenged with two strains isolated from the faeces of a diarrheic calf (B20a) or from the heart blood of a septicaemic calf (1404). All calves had diarrhoea which lasted until euthanasia in eight of them. In those calves, diarrhoea was correlated with the faecal excretion of the challenge strains. At necropsy, vascular congestion of the intestinal mucosa, hypertrophy of the mesenteric lymph nodes (MLN) and some congestion of the lungs were observed. Bacteriology confirmed the colonisation of the intestine by the challenge strains which were also recovered from the heart blood, the lungs and/or the liver. Histological sections confirmed enterocolitis, lymphadenitis and limited bronchopneumonia. In the intestinal tissue sections, bacteria testing positive in an in situ DNA hybridisation assay with a CNF2 probe were observed. Those results were confirmed by immunohistochemistry with a polyclonal anti-O78 and a monoclonal anti-F17b antisera. Three of the five control calves receiving either saline or a CNF À , F17a strain (25KH09) had no clinical signs or lesions. The other two presented a profuse liquid diarrhoea but those calves were positive for the presence of K99 þ E. coli. In this model, both NTEC2 strains were thus, able to colonise the intestine, to cause long-lasting diarrhoea and to invade the blood stream with localisation in various internal organs in colostrumrestricted conventional newborn calves. #

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Section: Methodsmentioning

confidence: 99%

Necrotoxigenic Escherichia coli type-2 invade and cause diarrhoea during experimental infection in colostrum-restricted newborn calves

Bost

Roels

Mainil

2001

Veterinary Microbiology

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“…One domain (adhesin), on the tip N/D Lintermans et al (1988Lintermans et al ( , 1991, Gerlach et al (1989), Bullitt & Makowski (1995), Adams et al (1997), Sebghati et al (1998), Soto & Hultgren (1999) Polyadhesive binding Thin flexible pili with a poorly defined diameter (2-4 nm) FGS: FaeE, FanE 5…”

Section: Introductionmentioning

confidence: 99%

FGL chaperone-assembled fimbrial polyadhesins: anti-immune armament of Gram-negative bacterial pathogens

Zavialov¹,

Zav’yalova²,

Korpela³

et al. 2007

FEMS Microbiol Rev

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This review summarizes the current knowledge on the structure, function, assembly, and biomedical applications of the family of adhesive fimbrial organelles assembled on the surface of Gram-negative pathogens via the FGL chaperone/usher pathway. Recent studies revealed the unique structural and functional properties of these organelles, distinguishing them from a related family, FGS chaperone-assembled adhesive pili. The FGL chaperone-assembled organelles consist of linear polymers of one or two types of protein subunits, each possessing one or two independent adhesive sites specific to different host cell receptors. This structural organization enables these fimbrial organelles to function as polyadhesins. Fimbrial polyadhesins may ensure polyvalent fastening of bacteria to the host cells, aggregating their receptors and triggering subversive signals that allow pathogens to evade immune defense. The FGL chaperone-assembled fimbrial polyadhesins are attractive targets for vaccine and drug design.

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“…The protein bands were stained with Coomassie blue. Strain K514(pPLHD2) (17) was used as a positive control, and K514(pUC18) (34) was used as a negative control. The structural subunit proteins of the thermoeluted fimbriae, produced by strains K514(pEOAL15) and K514(pEOAL17), migrated as a single band in SDS-PAGE with an apparent molecular mass of 20 kDa, as was observed for the F17 fimbriae (data not shown).…”

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confidence: 99%

“…The two F17-A-specific primers are located at positions 338 to 361 and 877 to 857 in the F17-A sequence (17). The three F17-G-specific primers are located at internal positions 322 to 346, 762 to 738, and 379 to 356 in the F17-G sequence (14).…”

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confidence: 99%

F17-like fimbriae from an invasive Escherichia coli strain producing cytotoxic necrotizing factor type 2 toxin

et al. 1994

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The F17b fimbriae encoded by the transmissible virulence plasmid Vir, also coding for cytotoxic necrotizing factor type 2, were characterized. A 5.7-kb region of Vir mediates in vitro N-acetylglucosamine-sensitive adhesion to calf intestinal villi. Sequence analysis revealed that this region codes for a structural subunit and an adhesin closely related to the F17-A and F17-G proteins encoded by the F17 fimbrial gene cluster. The F17b-A gene presents an open reading frame of 540 bp encoding a polypeptide of 180 amino acids with a putative signal peptide of 21 residues. The mature protein shows an identity of 74% with the F17-A structural subunit. This 20-kDa protein is recognized by antiserum directed against F17 fimbriae. The F17b-G gene shows an open reading frame of 1,029 bp encoding a polypeptide of 343 amino acids with a putative signal peptide of 22 residues. The F17b-G polypeptide exhibits 95% identity with the F17-G adhesin. The functional homology of the gene products was further confirmed by demonstrating that mutants in the F17-A gene can be complemented by the F17b-A gene and vice versa. These results prove that fimbriae belonging to the F17 family are also found on pathogenic Escherichia coli strains other than enterotoxigenic isolates producing heat-labile or heat-stable enterotoxin.

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Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the F17 fimbriae in bovine enterotoxigenic Escherichia coli

Cited by 61 publications

References 42 publications

Necrotoxigenic Escherichia coli type-2 invade and cause diarrhoea during experimental infection in colostrum-restricted newborn calves

Necrotoxigenic Escherichia coli type-2 invade and cause diarrhoea during experimental infection in colostrum-restricted newborn calves

FGL chaperone-assembled fimbrial polyadhesins: anti-immune armament of Gram-negative bacterial pathogens

F17-like fimbriae from an invasive Escherichia coli strain producing cytotoxic necrotizing factor type 2 toxin

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