Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau

Watts, M.; Munday, B. L.; Burke, Christopher M.

doi:10.1006/fsim.2000.0329

Cited by 42 publications

(30 citation statements)

References 34 publications

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“…Additional proteins in the vicinity of the 75 kDa major band were also observed in some aliquots eluted from the BSA-affinity column, or detected by western blot in the serum of several fish species used in this study. Evidence for the existence of distinct forms of H (Palenzuela et al 1996, Watts et al 2001, Jang et al 2004, Suresh Babu et al 2008) and L chain (Espelid & Grøntvedt 2003, Ishikawa et al 2004, Feng et al 2009) within teleost fish has already been reported and our data indicates that this could also be true for silver catfish and the other fish evaluated here. However, slight differences in the molecular weight of a protein might be attributed to different pattern of glycosylation.…”

Section: Discussionsupporting

confidence: 80%

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

Kreutz

Canova²,

Nied³

et al. 2016

Pesq. Vet. Bras.

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Knowledge on fish immunoglobulin (Ig) characteristics and the availability of monoclonal or polyclonal antibodies to fish Igs are essential to evaluate the humoral immune response and the Ig distribution on leukocyte cells. We demonstrated that silver catfish serum Ig is composed of one immunodominant H chain with approximately 75k Da and one L chain with approximately 28 kDa, similar to human IgM. Rabbit polyclonal antibodies to the catfish IgM-like Ig recognized both the H and L chain and were useful in developing an indirect ELISA to measure the production of antibodies in fish immunized with bovine serum albumin. Dot blot and western blot cross-reactivity studies indicated a wide degree of epitope sharing amongst Ig from several Siluriformes and Characiformes fish indigenous to Brazilian rivers. In these fish species, polyclonal antibodies reacted mostly with the H chain. The results presented here are central to the development of tools and strategies to investigate the antibody production to inoculated antigens and tissue distribution of Ig molecules in native fish species. Furthermore, because of the wide range of cross-reactivity, polyclonal antibodies to silver catfish IgM-like Ig might be used to develop immunoassays to measure the humoral immune response in other fish species.

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Section: Discussionsupporting

confidence: 80%

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

Kreutz

Canova²,

Nied³

et al. 2016

Pesq. Vet. Bras.

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“…MBP is an animal lectin that acts in pathogen clearance and possesses a high specificity for the mannose regions of IgM (Koppel et al, 1994). These two molecules have been used for affinity column-based IgM purification from fish sera (Suzuki et al, 1990;Al-Harbi et al, 2000;Watts et al, 2001;Crosbie and Nowak, 2002;Bromage et al, 2004), as has goat IgG (Palenzuela et al, 1996;Watts et al, 2001). In the present work, we found that protein A possessed a low affinity for olive flounder IgM versus the other capture ligands.…”

Section: Discussionmentioning

confidence: 59%

“…On the other hand, previous 2-DE experiments showed that the spots corresponding to the heavy chain of human IgM were found within a pI range of 5.6 to 6.4 at a molecular weight (MW) of 80-82 kDa (Al-Harbi et al, 2000;Watts et al, 2001;Crosbie and Nowak, 2002;Bromage et al, 2004;Kang et al, 2006;Palenzuela et al, 1996;Kaattari and Piganelli, 1996). The pI ranges and molecular weights of the heavy chain of IgM differed slightly in the emerald rockcod Ttrematomus bernacchii (pI range 4.0-6.0, MW 78 kDa) (Pucci et al, 2003), rainbow trout (pI 4.8-6.0, MW 76 kDa) and Arctic char Salvelinus alpinus (pI 5.0 -6.5, MW 76 kDa) (Hordvik et al, 2002), whereas the light chain spots in all three fish species were broadly distributed across a pI range of 4 to 7 (Hordvik et al, 2002;Pucci et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…A large variety of techniques have been used to purify Igs from fish serum, including precipitation in saturated ammonium sulfate, gel filtration, ion exchange chromatography and affinity chromatography. Affinity columns bound with specific ligands, such as protein A, mannan binding protein (MBP) and specific protein, have been widely used for purifying IgM from fish sera due to their methodological simplicity and specificity to IgM (Suzuki et al, 1990;Al-Harbi et al, 2000;Watts et al, 2001;Crosbie and Nowak, 2002;Bromage et al, 2004).…”

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confidence: 99%

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Purification of Two Different Immunoglobulins (Igs) from Olive Flounder Paralichthys olivaceus and Analysis of Lactococcus garvieae Antigens by the Igs

et al. 2007

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ABSTRACT-Immunoglobulins (Igs) were purified from sera obtained from olive flounder Paralichthys olivaceus immunized with goat IgG using immunoaffinity and mannan-binding protein (MBP) affinity columns and designated IMMIg and MBPIg respectively. SDS-PAGE and two-dimensional gel electrophoresis (2-DE) analyses demonstrated that the olive flounder serum contained at least two different types of Ig in terms of molecular weight and pI of heavy chain. 2-DE separation of the Igs followed by peptide mass fingerprinting using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) revealed that these two Igs were IgM and IgM precursor of olive flounder. Monoclonal antibodies (MAbs) were raised against the IMMIg and MBPIg and proteome analysis of Lactococcus garvieae using these MAbs and olive flounder immune sera demonstrated that IMMIg and MBPIg recognized different antigens of L. garvieae. This suggested that different Igs are possibly involved in protecting olive flounder against L. garvieae infection.

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“…The present results show that three doses of FPH in the diet could increase serum C3 Immunoglobulins are well recognized to provide protection in animals and humans against various diseases. IgM is a major component of the teleost humoral immune system (Watts et al, 2001;Cuesta et al, 2004). IgM is present in blood and other body fluids, and plays a role as an immune effector molecule (Ross et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.)

Tang

Zhao

et al. 2008

J. Zhejiang Univ. Sci. B

137

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Abstract:We investigated the effects of fish protein hydrolysate (FPH) on growth performance and humoral immune response of the large yellow croaker (Pseudosciaena crocea R.). One thousand and two hundred large yellow croakers [initial average weight: (162.75±23.85) g] were divided into four groups and reared in floating sea cages (3 m×3 m×3 m). The animals were fed with 4 diets: basal diet only (control) or diets supplemented with 5%, 10% and 15% (w/w) FPH. The results show that dietary FPH levels significantly influenced the growth and immunity of the large yellow croaker. Compared with the control group, total weight gain (TWG) in all treatment groups, relative weight gain (RWG) and specific growth rate (SGR) in fish fed with diets supplemented with 10% and 15% FPH were significantly increased (P<0.05). Similar results were observed in immune parameters [lysozyme activity, serum complements, immunoglobulin M (IgM)]. Lysozyme activity, complement C4 and IgM were also significantly increased (P<0.05) in fish fed with diets supplemented with 10% and 15% FPH, while complement C3 level was significantly increased (P<0.05) in all treatment groups. In general, with the supplementation of FPH, particularly at dose of 10%, the growth performance and immunity of the large yellow croaker can be improved effectively.

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Isolation and partial characterisation of immunoglobulin from southern bluefin tuna Thunnus maccoyii Castelnau

Cited by 42 publications

References 34 publications

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

Purification of Two Different Immunoglobulins (Igs) from Olive Flounder Paralichthys olivaceus and Analysis of Lactococcus garvieae Antigens by the Igs

Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.)

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