Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus

Luu, Lisa; Bown, Kevin; Palomar, Ana M.; Kazimírová, Mária; Bell‐Sakyi, Lesley

doi:10.1016/j.ttbdis.2020.101501

Cited by 11 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, a novel trypanosome designated as Trypanosoma sp. Bratislava1 from questing I. ricinus adults collected in SW Slovakia was isolated in tick cell culture and partially characterised (Luu et al 2020). The analyses have suggested that it may be a new species related to species detected in ticks in South America and Asia, and to Trypanosoma caninum isolated from dogs in Brazil.…”

Section: Non-zoonotic Microorganisms and Parasites Detected In Ticksmentioning

confidence: 98%

Ticks and their epidemiological role in Slovakia: from the past till present

et al. 2021

Self Cite

View full text Add to dashboard Cite

In Slovakia, 22 tick species have been found to occur to date. Among them, Ixodes ricinus, Dermacentor reticulatus, D. marginatus and marginally Haemaphysalis concinna, H. inermis and H. punctata have been identified as the species of public health relevance. Ticks in Slovakia were found to harbour and transmit zoonotic and/or potentially zoonotic agents such as tickborne encephalitis virus (TBEV), spirochaetes of the Borrelia burgdorferi sensu lato (s.l.) complex, the relapsing fever sprirochaete Borrelia miyamotoi, bacteria belonging to the orders Rickettsiales (Rickettsia spp., Anaplasma phagocytophilum, Neoehrlichia mikurensis), Legionellales (Coxiella burnetii), and Thiotrichales (Francisella tularensis), and Babesia spp. parasites (order Piroplasmida). Ixodes ricinus is the principal vector of the largest variety of microorganisms including viruses, bacteria and piroplasms. TBEV, B. burgdorferi s.l., rickettsiae of the spotted fever group, C. burnetii and F. tularensis have been found to cause serious diseases in humans, whereas B. miyamotoi, A. phagocytophilum, N. mikurensis, Babesia microti, and B. venatorum pose lower or potential risk to humans. Distribution of TBEV has a focal character. During the last few decades, new tick-borne encephalitis (TBE) foci and their spread to new areas have been registered and TBE incidence rates have increased. Moreover, Slovakia reports the highest rates of alimentary TBE infections among the European countries. Lyme borreliosis (LB) spirochaetes are spread throughout the distribution range of I. ricinus. Incidence rates of LB have shown a slightly increasing trend since 2010. Only a few sporadic cases of human rickettsiosis, anaplasmosis and babesiosis have been confirmed thus far in Slovakia. The latest large outbreaks of Q fever and tularaemia were recorded in 1993 and 1967, respectively. Since then, a few human cases of Q fever have been reported almost each year. Changes in the epidemiological characteristics and clinical forms of tularaemia have been observed during the last few decades. Global changes and development of modern molecular tools led to the discovery and identification of emerging or new tick-borne microorganisms and symbionts with unknown zoonotic potential. In this review, we provide a historical overview of research on ticks and tick-borne pathogens in Slovakia with the most important milestones and recent findings, and outline future directions in the investigation of ticks as ectoparasites and vectors of zoonotic agents and in the study of tick-borne diseases.Keywords Tick-borne pathogens . Tick-borne encephalitis virus . Borrelia . Anaplasma . Rickettsia . Coxiella burnetii . Francisella tularensis . Babesia

show abstract

Section: Non-zoonotic Microorganisms and Parasites Detected In Ticksmentioning

confidence: 98%

Ticks and their epidemiological role in Slovakia: from the past till present

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…After isolation, both species grew axenically in L-15B medium. Very recently, another trypanosome was isolated into ISE6 cells from questing I. ricinus and partially characterized [175]. Analysis of the resultant DNA sequences suggest that this trypanosome may be a new species closely related to several species or strains of trypanosomes isolated from, or detected in, ticks in South America and Asia [173,174,176], and to Trypanosoma caninum isolated from dogs in Brazil [177].…”

Section: Trypanosomamentioning

confidence: 99%

How relevant are in vitro culture models for study of tick-pathogen interactions?

Salata

Moutailler

Attoui

et al. 2021

Pathogens and Global Health

Self Cite

View full text Add to dashboard Cite

Although tick-borne infectious diseases threaten human and animal health worldwide, with constantly increasing incidence, little knowledge is available regarding vector-pathogen interactions and pathogen transmission. In vivo laboratory study of these subjects using live, intact ticks is expensive, labor-intensive, and challenging from the points of view of biosafety and ethics. Several in vitro models have been developed, including over 70 continuous cell lines derived from multiple tick species and a variety of tick organ culture systems, facilitating many research activities. However, some limitations have to be considered in the translation of the results from the in vitro environment to the in vivo situation of live, intact ticks, and vertebrate hosts. In this review, we describe the available in vitro models and selected results from their application to the study of tick-borne viruses, bacteria, and protozoa, where possible comparing these results to studies in live, intact ticks. Finally, we highlight the strengths and weaknesses of in vitro tick culture models and their essential role in tick-borne pathogen research.

show abstract

“…Questing (unfed) ticks, and ticks (engorged) collected on marsupial hosts [12,36] animals (e.g., vampire bats) that have recently fed on parasitaemic vertebrates [6]. Ticks have long been proposed as vectors of trypanosomes, but their importance in transmission cycles remains unclear [12][13][14][15][16]. Early microscopic morphological observations provided evidence for the presence of trypanosomes in a variety of tick species [17][18][19][20].…”

Section: Amblyomma Triguttatum Trypanosoma Copemani T Vegrandis T Noyesimentioning

confidence: 99%

Phylogenetic evidence for a clade of tick-associated trypanosomes

et al. 2023

View full text Add to dashboard Cite

Background Trypanosomes are protozoan parasites of vertebrates that are of medical and veterinary concern. A variety of blood-feeding invertebrates have been identified as vectors, but the role of ticks in trypanosome transmission remains unclear. Methods In this study, we undertook extensive molecular screening for the presence and genetic diversity of trypanosomes in field ticks. Results Examination of 1089 specimens belonging to 28 tick species from Europe and South America led to the identification of two new trypanosome strains. The prevalence may be as high as 4% in tick species such as the castor bean tick Ixodes ricinus, but we found no evidence of transovarial transmission. Further phylogenetic analyses based on 18S rRNA, EF1-α, hsp60 and hsp85 gene sequences revealed that different tick species, originating from different continents, often harbour phylogenetically related trypanosome strains and species. Most tick-associated trypanosomes cluster in a monophyletic clade, the Trypanosoma pestanai clade, distinct from clades of trypanosomes associated with transmission by other blood-feeding invertebrates. Conclusions These observations suggest that ticks may be specific arthropod hosts for trypanosomes of the T. pestanai clade. Phylogenetic analyses provide further evidence that ticks may transmit these trypanosomes to a diversity of mammal species (including placental and marsupial species) on most continents. Graphical Abstract

show abstract

Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus

Cited by 11 publications

References 37 publications

Ticks and their epidemiological role in Slovakia: from the past till present

Ticks and their epidemiological role in Slovakia: from the past till present

How relevant are in vitro culture models for study of tick-pathogen interactions?

Phylogenetic evidence for a clade of tick-associated trypanosomes

Contact Info

Product

Resources

About