Isolation and partial characterization of a polypeptide from Phoneutria nigriventer spider venom that relaxes rabbit corpus cavernosum in vitro

Rego, Evandro José Lima; Bento, A. C.; Lopes-Martins, Rodrigo Álvaro Brandão; Antunes, Edson; Novello, José Camillo; Marangoni, Sérgio; Giglio, José Roberto; Oliveira, Benedito; Nucci, Gilberto De

doi:10.1016/0041-0101(96)00060-8

Cited by 20 publications

(11 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These effects are abolished by pretreatment with 7-nitroindazole, a selective neuronal NO synthase inhibitor, indicating a role for NO in these responses (18). Increased NO formation and the resulting relaxation of corpus cavernosum smooth muscle (12,14,18) could possibly explain the long-lasting Table 1. Accuracy and intra-and inter-assay variation for the ELISA used to quantify P. nigriventer venom Venom concentration (ng/mL) Accuracy (%) (n = 6) Intra-assay CV (%) (n = 6)…”

Section: Discussionmentioning

confidence: 99%

“…When molested, Phoneutria spiders assume a very characteristic defensive posture and can jump up to 40 cm (1)(2)(3). Phoneutria nigriventer, which lives in central-western, southeastern, and southern Brazil, is responsible for most bites by this genus in humans (1)(2)(3)(4)(5), and its venom is the most studied of the six Phoneutria species identified in Brazil (5,(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Systemic envenomation caused by the wandering spiderPhoneutria nigriventer, with quantification of circulating venom

Bucaretchi

Mello

Vieira

et al. 2008

Clinical Toxicology

View full text Add to dashboard Cite

Introduction. Bites by Phoneutria spp. spiders are common in Brazil, although only 0.5-1% result in severe envenomation, with most of these occurring in children. Cases of systemic envenomation in adults are very unusual, and no serum venom levels have been previously quantified in these cases. Case report. A 52-year-old man was bitten on the neck by an adult female Phoneutria nigriventer. Immediately after the bite, there was intense local pain followed by blurred vision, profuse sweating, tremors, and an episode of vomiting; 1-2 h post bite the patient showed agitation and a blood pressure of 200/130 mmHg, and was given captopril and meperidine. Upon admission to our service 4 h post bite (time zero -T0), his blood pressure was 130/80 mmHg with a heart rate of 150 beats/min, mild tachypnea, agitation, cold extremities, profuse sweating, generalized tremors, and priapism. The patient was treated with antivenom, local anesthetic, and fluid replacement. Most of the systemic manifestations disappeared within 1 h after antivenom. Laboratory blood analyses at T0, T1, T6, T24, and T48 detected circulating venom by ELISA only at T0, before antivenom infusion (47.5 ng/mL; cut-off, 17.1 ng/mL); his serum blood sugar was 163 mg/dL at T0. The patient was discharged on the second day with a normal arterial blood pressure and a follow-up evaluation revealed no sequelae. Conclusion. This is the first report of confirmed moderate/severe envenoming in an adult caused by P. nigriventer with the quantification of circulating venom.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systemic envenomation caused by the wandering spiderPhoneutria nigriventer, with quantification of circulating venom

Bucaretchi

Mello

Vieira

et al. 2008

Clinical Toxicology

View full text Add to dashboard Cite

show abstract

“…It is interesting to note that tissue kallikreins obtained from porcine pancreas and rabbit urinary tract significantly increase plasma exudation in the rabbit skin, whereas rabbit plasma kallikrein fails to evoke oedema formation [256]. It is also worth mentioning that PNV activates tissue kallikrein-kininogen-kinin system in the rabbit corpus cavernosum in vitro, leading to the release of the vasorelaxant mediator NO [257,258]. This is consistent with the findings that activation of BK B 2 receptors in human erectile tissue leads to release of NO [259].…”

Section: Activation Of the Kallikrein-kininogen-kinin Systemmentioning

confidence: 97%

Pro and Antiinflammatory Properties of Toxins from Animal Venoms

Farsky

Antunes²,

Mello³

2005

CDTIA

View full text Add to dashboard Cite

Accidents evoked by venomous animals are common in tropical regions. In Brazil, envenomation evoked by snakes, spiders and scorpions are an important public health problem. Their venoms are composed of a great number of toxins, which are capable of acting on tissue and plasma components with consequent toxic and pharmacological effects. On the other hand, the diversity of venom composition makes them important source of toxins that can be employed as scientific tools. Here we describe the mechanisms of anti and pro-inflammatory properties of toxins of Bothrops and Crotalus genus snakes and Loxosceles and Phoneutria genus spider venoms. The emphasis was to summarise, both in vivo and in vitro, studies that focused on the action of phospholipases, metalloproteinases and sphingomyelinase D on vascular and cellular aspects of the process as well as the complex network of chemical mediators involved.

show abstract

“…Although the major toxins of spider venoms are neurotoxic peptides, which act on a vast array of ion channels (Kushmerick et al, 1999;Escoubas et al, 2000;Gomez et al, 2002;Matavel et al, 2002;King and Hardy, 2013), non-neurotoxic peptides and also non-peptidic molecules have been described (Bento et al, 1993;Marangoni et al, 1993;Rego et al, 1996;Rash and Hodgson, 2002).…”

Section: Introductionmentioning

confidence: 98%

ADP is a vasodilator component from Lasiodora sp. mygalomorph spider venom

Horta

Rezende

Oliveira-Mendes

et al. 2013

Toxicon

View full text Add to dashboard Cite

Members of the spider genus Lasiodora are widely distributed in Brazil, where they are commonly known as caranguejeiras. Lasiodora spider venom is slightly harmful to humans. The bite of this spider causes local pain, edema and erythema. However, Lasiodora sp. spider venom may be a source of important pharmacological tools. Our research group has described previously that Lasiodora sp. venom produces bradycardia in the isolated rat heart. In the present work, we sought to evaluate the vascular effect of Lasiodora sp. venom and to isolate the vasoactive compounds from the venom. The results showed that Lasiodora spider venom induced a concentration-dependent vasodilation in rat aortic rings, which was dependent on the presence of a functional endothelium and abolished by the nitric oxide synthase (NOS) inhibitor L-NAME. Western blot experiments revealed that the venom also increased endothelial NOS function by increasing phosphorylation of the Ser¹¹⁷⁷ residue. Assay-directed fractionation isolated a vasoactive fraction from Lasiodora sp. venom. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) assays identified a mixture of two compounds: adenosine diphosphate (ADP, approximately 90%) and adenosine monophosphate (AMP, approximately 10%). The vasodilator effects of Lasiodora sp. whole venom, as well as ADP, were significantly inhibited by suramin, which is a purinergic P2-receptor antagonist. Therefore, the results of the present work indicate that ADP is a main vasodilator component of Lasiodora sp. spider venom.

show abstract

Isolation and partial characterization of a polypeptide from Phoneutria nigriventer spider venom that relaxes rabbit corpus cavernosum in vitro

Cited by 20 publications

References 16 publications

Systemic envenomation caused by the wandering spiderPhoneutria nigriventer, with quantification of circulating venom

Systemic envenomation caused by the wandering spiderPhoneutria nigriventer, with quantification of circulating venom

Pro and Antiinflammatory Properties of Toxins from Animal Venoms

ADP is a vasodilator component from Lasiodora sp. mygalomorph spider venom

Contact Info

Product

Resources

About