Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.

Abstract

The alcohol dehydrogenase (ADH; alcohol: NAD+ oxidoreductase, EC 1.1.1.1) gene (Adh)

Restriction endonuclease map variation and gene activity in the ADH region in a population of Drosophila melanogaster

“…(1982), not dissimilar from that of the sAF-2 clone of Goldberg (1980); this is the restriction map presented in Fig. 1.…”

“…This is not surprising since ADH activity is controlled in this population (Barnes and Birley, 1978) as well as in other populations (McDonald and Ayala, 1978;Laurie-Ahlberg, Maroni, Bewley, Lucchesi and Weir, 1980), by loci which are located upon different chromosomes (I and III) from that upon which the Adh structural gene (chromosome II) is located. (Goldberg, 1980) and Langley Genotype-environment interaction for ADH activity has been previously assessed in the set of inbred lines by the methods of biometrical genetics (Perkins and Jinks, 1968;Pooni and Jinks, 1980). In summary, the methods compare the performance of the set of inbred lines in a set of different environments, the measure of various environments being in this case purely biological and provided by the average performance of all lines.…”

“…The pBR322 based on sAS-1 plasmid (Goldberg, 1980) was labelled with a32P dCTP, 3000Ci mM' (Amersham International) by nick-translation (Rigby, Diekmann, Rhodes and Berg, 1977) and hybridisation carried out in the presence of dextran sulphate (Wahl, Stern and Stark, 1979). Autoradiography followed with Kodak X-Omat RP film for 24-48 hours at -70°C.…”

Restriction endonuclease variation in the region of the alcohol dehydrogenase gene: a comparison of null and normal alleles from natural populations of Drosophila melanogaster

“…(1984). The sAF-2 plasmid (Goldberg, 1980), which includes within an 11.5 kb insert the coding sequence of the D. rne!anogaster Adh gene together with 10 kb of flanking DNA, was labelled with [a-32P]-dCTPby nick translation (Rigby et a!., 1977). Autoradiography was carried out at -70°C on Kodak film (XRP-1) using intensifying screens.…”

Isolation and characterization of the glucose-6-phosphate dehydrogenase gene of Drosophila melanogaster

“…A subclone, SAC-~ (Goldberg, 1980), containing the Adh gene of f). ~eia~g~ter was obtained from Dr. J.C. Lucchesi, University of North Carolina.…”