Isolation and Partial Characterization of Immunostimulating Polysaccharides from Imperata cylindrica

Abstract

The water-soluble crude extract prepared from Imperata cylindrica (Beauv.) was investigated for its immunomodulating activity. A set of polysaccharides with high molecular weights has been isolated by fractionation using gel filtration and anion-exchange chromatography. Each step of purification was monitored by bioassays. The presence of six monosaccharides has been established by chemical analysis. Quantitative analysis showed that the ratio of these monosaccharides differed from one polysaccharide to another. The crude extract as well as some of the purified polysaccharides enhance the proliferation of murine splenocytes.

Anti-Cancer Effects of Imperata cylindrica Leaf Extract on Human Oral Squamous Carcinoma cell line SCC-9 in Vitro

“…Its diuretic properties have been reported by Doan et al, 1992 andShah et al, 2012. In addition, it is reported to possess immunomodulatory (Pinilla and Luu, 1999) as well as neuroprotective (Yoon et al, 2006) activities. I. cylindrica is one of the important herbs used in ayurvedic formulations possessing significant antioxidant activities (Sushama and Nishteswar, 2014;Pragya et.al., 2015).…”

Immunomodulation and antitumor activity by a polysaccharide–protein complex from Lycium barbarum

“…Chemical composition of LBP 3p was 63.56% neutral sugars, 24.80% acidic sugars and 7.63% proteins as determined by phenol -H 2 SO 4 [8], carbazole [9] and Lowry method [10], respectively. It contained six monosaccharides: galactose, glucose, rhamnose, arabinose, mannose, xylose and the molar ratio was 1:2.12:1.25:1.10:1.95:1.76, respectively, by gas chromatography [11]. The molecular weight of LBP 3p was 1.57 Â 10 5 by laser light scattering [12].…”

Bioactive Polysaccharides from Traditional Chinese Medicine Herbs as Anticancer Adjuvants

“…(Wang et al, 1992) Achyranthes bidentata (Li and Li, 1997) Aloe spp. (Zhang and Tizard, 1996) Atractylodes (Inagaki et al, 2001) Beniscasa cerifera (Kumazawa et al, 1985) Cinnamomum cortex (Haranaka et al, 1985) a Curcuma zedoaria (Kim et al, 2000) Codonopsis pilosula (Wang et al, 1996) Dipsacus asperoides (Zhang et al, 1997) Epimedium sagittatum (Liu et al, 1991) Imperata cylindrica (Pinilla and Luu, 1999) Isatis indigotica (Xu and Lu, 1991) Malva verticillata (Gonda et al, 1990) Panax notoginseng (Gao et al, 1996) b Pseudostellaria heterophylla (Wong et al, 1992) Radix bupleuri (Geng and Chen, 1989) Radix glycyrrhizia (Nose et al, 1998) Radix hadysari (Lan et al, 1987) c Radix pseuoginseng (Lin, 1988) Radix Rehmannia (Xu, 1992) Salvia miltiorrhiza (Hromakova et al, 1999) Zizyphi fructus (Yamaoka et al, 1996) a Haranaka K, Satomi N, Sakurai A, Haranka R, Okada N, Kobayashi M. Antitumor activities and tumor necrosis factor producibility of traditional Chinese medicines and crude drugs.…”