Isolation and purification of carbazole contained in anthracene slag by extraction combined with medium pressure liquid chromatography

Ma, Zhi-Hao; Wei, Xian‐Yong; Zhou, Ming-yao; Liu, Guanghui; Liu, Fang-Jing; Liu, Zhongqiu; Yu, Xinyue; Zong, Zhi‐Min

doi:10.1016/j.cjche.2018.12.012

Cited by 10 publications

(4 citation statements)

References 38 publications

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“…Instead, target compounds must first be enriched from the crude extracts via sample pretreatment, while non-target components should be eliminated. Based on the principle of analytical liquid chromatography, which takes advantage of the fact that different substances have different partition coefficients in a system consisting of a stationary phase and a mobile phase, medium pressure liquid chromatography (MPLC) represents an effective pretreatment approach [21][22][23]. As such, the preferred approach to the large-scale preparation of high-purity bioactive compounds is a combination of MPLC and prep-HPLC [24,25].…”

Section: Introductionmentioning

confidence: 99%

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Fang

et al. 2022

Nutrients

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In this study, a 100 g sample of Saxifraga atrata was processed to separate 1.3 g of 11-O-(4′-O-methylgalloyl)-bergenin (Fr1) after 1 cycle of MCI GEL® CHP20P medium pressure liquid chromatography using methanol/water. Subsequently, COX-2 affinity ultrafiltration coupled with reversed-phase liquid chromatography was successfully used to screen for potential COX-2 ligands in this target fraction (Fr1). After 20 reversed-phase liquid chromatography runs, 74.1 mg of >99% pure 11-O-(4′-O-methylgalloyl)-bergenin (Fr11) was obtained. In addition, the anti-inflammatory activity of 11-O-(4′-O-methylgalloyl)-bergenin was further validated through molecular docking analyses which suggested it was capable of binding strongly to ALOX15, iNOS, ERBB2, SELE, and NF-κB. As such, the AA metabolism, MAPK, and NF-κB signaling pathways were hypothesized to be the main pathways through which 11-O-(4′-O-methylgalloyl)-bergenin regulates inflammatory responses, potentially functioning by reducing pro-inflammatory cytokine production, blocking pro-inflammatory factor binding to cognate receptors and inhibiting the expression of key proteins. In summary, affinity ultrafiltration-HPLC coupling technology can rapidly screen for multi-target bioactive components and when combined with molecular docking analyses, this approach can further elucidate the pharmacological mechanisms of action for these compounds, providing valuable information to guide the further development of new multi-target drugs derived from natural products.

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Section: Introductionmentioning

confidence: 99%

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Fang

et al. 2022

Nutrients

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“…Although MPLC can be used to isolate a compound [77], it is generally used to enrich bioactive compounds (secondary metabolites) from natural products before further purification because of its low cost, high sample load, and high throughput [78]. Therefore, MPLC can be used to remove non-target compounds.…”

Section: Medium Pressure Liquid Chromatographymentioning

confidence: 99%

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Susanti,

Pratiwi,

Rosandi

et al. 2024

Plants

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In recent years, discovering new drug candidates has become a top priority in research. Natural products have proven to be a promising source for such discoveries as many researchers have successfully isolated bioactive compounds with various activities that show potential as drug candidates. Among these compounds, phenolic compounds have been frequently isolated due to their many biological activities, including their role as antioxidants, making them candidates for treating diseases related to oxidative stress. The isolation method is essential, and researchers have sought to find effective procedures that maximize the purity and yield of bioactive compounds. This review aims to provide information on the isolation or separation methods for phenolic compounds with antioxidant activities using column chromatography, medium-pressure liquid chromatography, high-performance liquid chromatography, counter-current chromatography, hydrophilic interaction chromatography, supercritical fluid chromatography, molecularly imprinted technologies, and high-performance thin layer chromatography. For isolation or purification, the molecularly imprinted technologies represent a more accessible and more efficient procedure because they can be applied directly to the extract to reduce the complicated isolation process. However, it still requires further development and refinement.

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“…Moreover, the petroleum ether (PE)-extractable portion of HTCT was isolated by equimolar CC/MA to obtain carbazole, quinoline, and benzo[ h ]quinoline in our laboratory, while 57.2% total NCAs recovery was acquired at DES/oil mass ratio of 1:2 and 35 °C for 30 min . However, the purity of product enriched by ILs/DESs does not satisfy the requirements as a chemical, and the column chromatography was used to obtain carbazole, quinoline, and benzo[ h ]quinoline with the relative content >90%.…”

Section: Separation Of Ncas By Ils/dessmentioning

confidence: 99%

Overview: Effective Separation of Oxygen-, Nitrogen-, and Sulfur-Containing Aromatics in High-Temperature Coal Tar by Ionic Liquids and Deep Eutectic Solvents: Experimental and Computational

Yan

Wei

Wang

et al. 2022

Ind. Eng. Chem. Res.

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Heteroatom-containing aromatics (HACAs), such as oxygen-containing aromatics (OCAs), nitrogen-containing aromatics (NCAs), and sulfur-containing aromatics (SCAs), in high-temperature coal tar (HTCT) can be regarded as valuable chemicals. Ionic liquids (ILs)/deep eutectic solvents (DESs) play a crucial role for selectively extracting and purifying HACAs from HTCT, which can be attributed to the complex interactions, especially hydrogen bonds, between the ILs/DESs and HACAs. This paper mainly summarizes the separation of HACAs from a model oil and raw oil with ILs/DESs, the structural effect of ILs/ DESs on HACA separation, as well as the interactions between ILs/DESs and different solutes by experimental and computational chemistry. Subsequently, HACA separation with ILs/DESs from HTCT was summarized. This review was expected to screen and develop suitable ILs/ DESs with high selectivities to OCAs, NCAs, SCAs, and their homologues.

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Isolation and purification of carbazole contained in anthracene slag by extraction combined with medium pressure liquid chromatography

Cited by 10 publications

References 38 publications

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Overview: Effective Separation of Oxygen-, Nitrogen-, and Sulfur-Containing Aromatics in High-Temperature Coal Tar by Ionic Liquids and Deep Eutectic Solvents: Experimental and Computational

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