Isolation and simultaneous determination of three biologically active flavonoids from some indigenous <i>Cordia</i> species by thin-layer chromatography with UV absorption densitometry method

Nariya, Pankajkumar B.; Shukla, VJ; Acharya, Rabinarayan; Nariya, Mukeshkumar

doi:10.1556/1006.2017.30.4.5

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…The relative standard deviation was calculated for the peak areas of quercetin (Sigma-Aldrich, Berlin, Germany) and kaempferol (Sigma-Aldrich, Berlin, Germany) by five chromatograms, and the deviation did not exceed 0.5%. The efficiency of the chromatographic column calculated according to the standards of quercetin and kaempferol amounted to more than 10,000 theoretical plates [ 60 , 61 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation of the Main Biologically Active Substances and Phytochemical Analysis of Ginkgo biloba Callus Culture Extracts

Сухих

Larichev

et al. 2023

Molecules

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The work reveals the results of studying the content of biologically active substances in samples of extracts of Ginkgo biloba callus cultures. Callus cultures grown in vitro on liquid nutrient media were the objects of the study. Considering various factors affecting the yield of the target components during extraction, the volume fraction of the organic modifier in the extracting mixture, the temperature factor, and the exposure time were identified as the main ones. The maximum yield of extractive substances (target biologically active substances with a degree of extraction of at least 50%) from the samples of callus culture extracts was detected at a ratio of extragent of 70% ethanol, a temperature of 50 °C, and exposure time of 6 h. Flavonoids, such as luteolin, quercetin, isoramentin, kaempferol, and amentoflavone, were isolated in the extract samples. As a result of column chromatography, fractions of individual biologically active substances (bilobalide, ginkgolide A, B, and C) were determined. The proposed schemes are focused on preserving the nativity while ensuring maximum purification from associated (ballast) components. Sorbents (Sephadex LH-20, poly-amide, silica gel) were used in successive stages of chromatography with rechromatography. The degree of purity of individually isolated substances was at least 95%.

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Section: Methodsmentioning

confidence: 99%

Isolation of the Main Biologically Active Substances and Phytochemical Analysis of Ginkgo biloba Callus Culture Extracts

Сухих

Larichev

et al. 2023

Molecules

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Quinones from Cordia species from 1972 to 2023: isolation, structural diversity and pharmacological activities

Dongmo Zeukang,

Kalinski,

Tembeni

et al. 2023

Nat. Prod. Bioprospect.

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Plants of the genus Cordia (Boraginaceae family) are widely distributed in the tropical regions of America, Africa, and Asia. They are extensively used in folk medicine due to their rich medicinal properties. This review presents a comprehensive analysis of the isolation, structure, biogenesis, and biological properties of quinones from Cordia species reported from 1972 to 2023. Meroterpenoids were identified as the major quinones in most Cordia species and are reported as a chemotaxonomic markers of the Cordia. In addition to this property, quinones are reported to display a wider and broader spectrum of activities, are efficient scaffold in biological activity, compared to other classes of compounds reported in Cordia, hence our focus on the study of quinones reported from Cordia species. About 70 types of quinones have been isolated, while others have been identified by phytochemical screening or gas chromatography. Although the biosynthesis of quinones from Cordia species is not yet fully understood, previous reports suggest that they may be derived from geranyl pyrophosphate and an aromatic precursor unit, followed by oxidative cyclization of the allylic methyl group. Studies have demonstrated that quinones from this genus exhibit antifungal, larvicidal, antileishmanial, anti-inflammatory, antibiofilm, antimycobacterial, antioxidant, antimalarial, neuroinhibitory, and hemolytic activities. In addition, they have been shown to exhibit remarkable cytotoxic effects against several cancer cell lines which is likely related to their ability to inhibit electron transport as well as oxidative phosphorylation, and generate reactive oxygen species (ROS). Their biological activities indicate potential utility in the development of new drugs, especially as active components in drug-carrier systems, against a broad spectrum of pathogens and ailments. Graphical Abstract

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Recent advances in methods of extraction, pre-concentration, purification, identification, and quantification of kaempferol

Norouzi,

Dastan,

Abdullah

et al. 2024

Journal of Chromatography A

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Isolation and simultaneous determination of three biologically active flavonoids from some indigenous Cordia species by thin-layer chromatography with UV absorption densitometry method

Cited by 5 publications

References 13 publications

Isolation of the Main Biologically Active Substances and Phytochemical Analysis of Ginkgo biloba Callus Culture Extracts

Isolation of the Main Biologically Active Substances and Phytochemical Analysis of Ginkgo biloba Callus Culture Extracts

Quinones from Cordia species from 1972 to 2023: isolation, structural diversity and pharmacological activities

Recent advances in methods of extraction, pre-concentration, purification, identification, and quantification of kaempferol

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