Isolation and structural studies of the intact scrapie agent protein

Bolton, David; Bendheim, Paul E.; Marmorstein, Alan D.; Potempska, Anna

doi:10.1016/0003-9861(87)90380-8

Cited by 142 publications

(90 citation statements)

References 44 publications

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“…Our results differ widely from those of , who report only 7.2 lag ofTSE-specific amyloid per brain. However, they are comparable to those of Bolton et al (1987Bolton et al ( , 1991a, who report 25-80 lag of disease-specific amyloid per gram of tissue in purified fractions from hamster brains containing 15-30 % of the scrapie infectivity in the starting material.…”

Section: Discussionsupporting

confidence: 88%

“…This amyloid protein is derived from a highly conserved host-encoded precursor, a cellular glycoprotein (tool. mass 33-35 kDa, also designated PrP c) (Oesch et al, 1985;Robakis et al, 1986;Locht et al, 1986;Basler et al, 1986;Bolton et al, 1987;Bendheim et al, 1988) located mainly at the surface of nerve cells (Stahl et al, 1987(Stahl et al, , 1990a. During the course of a TSE infection, an as yet unknown post-translational event (Borchelt et al, 1990) converts this cellular protein into a disease-specific pathological isoform (also termedpathological isoforms of the protein differ in their sensitivity towards proteolytic activity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Western blot mapping of disease-specific amyloid in various animal species and humans with transmissible spongiform encephalopathies using a high-yield purification method

Beekes

Baldauf

Cassens

et al. 1995

Journal of General Virology

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Western blot mapping of disease-specific amyloid in various animal species and humans with transmissible spongiform encephalopathies using a high-yield purification method

Beekes

Baldauf

Cassens

et al. 1995

Journal of General Virology

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“…There is abundant evidence that Sp33-37 plays an essential role in initiating the disease and promoting synthesis of the scrapie agent (Bolton et al, 1982(Bolton et al, , 1987McKinley et al, 1983;Gabizon et al, 1987Gabizon et al, , 1988Bendheim et al, 1988 b;Carp et al, 1989;Safar et al, 1990;Brown et al, 1990). Sp33-37 aggregates into readily sedimentable forms during isolation.…”

Section: Introductionmentioning

confidence: 99%

Copurification of Sp33-37 and Scrapie Agent from Hamster Brain Prior to Detectable Histopathology and Clinical Disease

Bolton¹,

Rudelli²,

Currie³

et al. 1991

Journal of General Virology

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Studies were conducted to determine whether accumulation of the scrapie agent protein Sp33-37 in brain correlated with the appearance of the scrapie agent or with pathology. The concentrations of the scrapie agent and Sp33-37 were measured in purified fraction P5 isolated from hamster brains at weekly intervals after inoculation. The scrapie agent concentration in fraction P5 was approximately 10 -1 LDs0/g brain 1 day post-inoculation and increased to 10 9.4 LDJg at day 77. Sp33-37 was first detected in P5 at day 21, when the agent titre was 10 3.9 LDs0/g. Sp33-37 concentration increased in concert with the scrapie agent concentration, although the apparent rate of increase was somewhat lower for the protein than for the agent. The histopathological evidence of disease, consisting of mild vacuolation and gliosis, was first seen at 35 days, but was not conspicuous until 49 to 56 days postinoculation. Vacuolation and gliosis increased until termination of the experiment at day 77. Amyloid plaques were first detected at 56 days and were widespread at day 77. Clinical disease was first seen in these animals at day 66, with an average onset at day 71. Control animals inoculated with buffer alone showed some mild gliosis, but were otherwise normal. The fact that Sp33-37 purified with the scrapie agent isolated from brain 14 days prior to detectable (light microscopic) pathology supports the theory that Sp33-37 is the major structural component of the scrapie agent and not solely a product of the pathology.

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“…Although the theory remains controversial, evidence in support of the protein-only model is substantial (Bolton et al, , 1987(Bolton et al, , 1991McKinley et al, 1983 ;Prusiner et al, 1984 ;Hsiao et al, 1989Hsiao et al, , 1990Scott et al, 1989Scott et al, , 1993Meyer et al, 1991 ;Bu$ eler et al, 1992).…”

mentioning

confidence: 99%

“…Thirty-four Syrian hamsters (virus-free weanling females) were inoculated in the peritoneal cavity with 50 µl 263K scrapie prions (1 : 100 dilution), purified as described previously (Bolton et al, 1987 ;Bendheim et al, 1988). Three hamsters were humanely killed by CO # asphyxiation on each of the days indicated in Fig.…”

mentioning

confidence: 99%

Prion distribution in hamster lung and brain following intraperitoneal inoculation.

Bolton¹

1998

Journal of General Virology

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Prion titres were measured in the lungs and brains of Syrian hamsters after intraperitoneal inoculation with sucrose gradient-purified 263K prions (" 10 8 LD 50 ). Prions were detected in the lung of one hamster on day 7, but were not detected in the lungs of any other hamster until day 71. Prions were detected in the lungs of all hamsters sampled thereafter but titres remained low through day 127. Prions were first detected in the brain on day 35 and brain titres increased exponentially until day 127 with a doubling time of about 4n5 days. On day 133, titres averaged 10 8n0 LD 50 /g in brain and 10 5n0 LD 50 /g in lung. Two out of the five remaining hamsters were clinically normal but prion titres were not significantly different from those in the clinically affected hamsters. Thus, significant prion titres may be found outside the CNS in clinically normal hamsters.

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Isolation and structural studies of the intact scrapie agent protein

Cited by 142 publications

References 44 publications

Western blot mapping of disease-specific amyloid in various animal species and humans with transmissible spongiform encephalopathies using a high-yield purification method

Western blot mapping of disease-specific amyloid in various animal species and humans with transmissible spongiform encephalopathies using a high-yield purification method

Copurification of Sp33-37 and Scrapie Agent from Hamster Brain Prior to Detectable Histopathology and Clinical Disease

Prion distribution in hamster lung and brain following intraperitoneal inoculation.

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